A5045683103- melanin and skin pigmentation
- Malaria Research and Control
- Computational Drug Discovery Methods
- Genetic and phenotypic traits in livestock
- Molecular Biology Techniques and Applications
- Cell Adhesion Molecules Research
- HIV Research and Treatment
- Genetics, Bioinformatics, and Biomedical Research
- Veterinary Equine Medical Research
- Mosquito-borne diseases and control
- RNA regulation and disease
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Skin and Cellular Biology Research
- Plant Gene Expression Analysis
- Genetic Mapping and Diversity in Plants and Animals
- Aquaculture disease management and microbiota
- Wound Healing and Treatments
- Cellular Mechanics and Interactions
- Plant and fungal interactions
- Global Health and Surgery
- Innovation and Socioeconomic Development
- Pharmacology and Obesity Treatment
- Heart Rate Variability and Autonomic Control
- Women's cancer prevention and management
Menlo School
2020-2023
Etalon (Germany)
2023
University of Notre Dame
2019-2022
North Shore Medical Center
2022
Boston Medical Center
2019-2021
Ambergen (United States)
2010
Beth Israel Deaconess Medical Center
2006-2008
Harvard University
2008
The simian immunodeficiency viruses (SIV) naturally infect a wide range of African primates, including green monkeys (AGM). Despite moderate to high levels plasma viremia in infected AGM, infection is not associated with immunodeficiency. We recently reported that SIVagmVer90 isolated from vervet AGM induced AIDS following experimental inoculation pigtailed macaques. goal the present study was evaluate replication this isolate two species sabaeus (Chlorocebus sabaeus) and vervets (C....
ABSTRACT Previously we have shown that CD8 + T cells are critical for containment of simian immunodeficiency virus (SIV) viremia and rapid profound depletion CD4 occurs in the intestinal tract acutely infected macaques. To determine impact SIV-specific T-cell responses on magnitude depletion, investigated effect lymphocyte during primary SIV infection subsets function peripheral blood, lymph nodes, tissues. In lymphocyte-depletion changed dynamics loss, resulting a more pronounced loss 2...
Breast cancer is the most diagnosed and second leading cause of deaths in U.S. female population. An estimated 5 to 10 percent all breast cancers are inherited, caused by mutations susceptibility genes (BRCA1/2). As many as 90% nonsense mutations, causing a truncated polypeptide product. A popular low cost method mutation detection has been protein truncation test (PTT), where target regions BRCA1/2 PCR amplified, transcribed/translated cell-free synthesis system analyzed for polypeptides...
Abstract Background Tracking and understanding artemisinin resistance is key for preventing global setbacks in malaria eradication efforts. The ring-stage survival assay (RSA) the current gold standard vitro phenotyping. However, RSA has several drawbacks: it relatively low throughput, high variance due to microscopy readout, correlates poorly with benchmark vivo resistance, patient clearance half-life post-artemisinin treatment. Here a modified presented, extended Recovery Ring-stage...
Data for this publication is deposited on GenBank, accession number ON381714. Appendix S1 Veterinary Patient Report the affected foal. S2 ConSurf analysis results NM_001163874.1 and respective protein NP_001157346.1. Please note: The publisher not responsible content or functionality of any supporting information supplied by authors. Any queries (other than missing content) should be directed to corresponding author article.
Here, we investigated the containment of virus replication in simian immunodeficiency (SIV) infection by CD8(+) lymphocytes. Escape mutations Mamu-A*01 epitopes appeared first SIV Tat TL8 and then Gag p11C. The appearance escape p11C was coincident with compensatory changes outside epitope. Eliminating lymphocytes from rhesus monkeys during primary resulted more rapid disease progression that associated preservation canonical epitopes. These results confirm importance cytotoxic T cells...
Over 30 polymorphisms in the KIT Proto-Oncogene Receptor Tyrosine Kinase (KIT) gene have been implicated white spotting patterns ranging from small areas to full dermal depigmentation horse. We performed a candidate-gene exon sequencing approach on and MITF, 2 known causatives of patterns, within families horses unknown spotting. Family 1 (Fam1, N = 5) consisted Quarter Horse stallion 4 offspring with pattern legs, lower ventral, head regions jagged borders, almost complete white. The second...
Over 40 identified genetic variants contribute to white spotting in the horse. White markings and are under selection for their impact on economic value of an equine, yet many phenotypes have unknown basis. Previous studies also demonstrate interaction between MC1R ASIP pigmentation loci associated with KIT MITF. We investigated two stallions presenting a phenotype cause. Exon sequencing MITF candidate genes missense variant (rs1140732842, NC_009146.3:g.79566881T>C, p.T391A) predicted by...
Abstract Background Tracking and understanding artemisinin resistance is key for preventing global setbacks in malaria eradication efforts. The ring-stage survival assay (RSA) the current gold standard vitro phenotyping. However, RSA has several drawbacks: it relatively low throughput, high variance due to microscopy readout, correlates poorly with benchmark vivo resistance, patient clearance half-life post-artemisinin treatment. Here a modified presented, extended Recovery Ring-stage...
Abstract Background The cyclical nature of gene expression in the intraerythrocytic development cycle (IDC) malaria parasite, Plasmodium falciparum , confounds accurate detection specific transcriptional differences, e.g. as provoked by drug resistance. In lab-based studies, P. cultures are synchronized to remove this confounding factor, but rapid emerging resistance artemisinin therapies requires analysis transcriptomes extracted directly from clinical samples. Here we propose use...
Mutations in KIT, a gene that influences melanoblast migration and pigmentation, often result mammalian white spotting. As of February 2023, over 30 KIT variants associated with spotting were documented Equus caballus (horse). Here we report an association increased on the skin coat variant 5’UTR (rs1149701677: g.79,618,649A>C). Horses possessing at least one alternate allele demonstrate phenotypic characteristics similar to other mutations: clear borders around unpigmented regions body,...
Mutations causing depigmentation are relatively common in Equus caballus (horse). Over 40 alleles multiple genes associated with increased white spotting (as of February 2023). The splashed phenotype, a coat pattern described as appearing like the horse has been paint, was previously variants PAX3 and MITF genes. Both encode transcription factors known to control melanocyte migration pigmentation. We report two novel mutations, stop-gain mutation (XM_005610643.3:c.927C>T, ECA6:11,196,181,...
Abstract Background Tracking and understanding artemisinin resistance is key for preventing global setbacks in malaria eradication efforts. The ring-stage survival assay (RSA) the current gold standard vitro phenotyping. However, RSA has several drawbacks: it relatively low throughput, high variance due to microscopy readout, correlates poorly with benchmark vivo resistance, patient clearance half-life post-artemisinin treatment. Here a modified presented, extended Recovery Ring-stage...
Abstract Background Tracking and understanding artemisinin resistance is key for preventing global setbacks in malaria eradication efforts. The ring-stage survival assay (RSA) the current gold standard vitro phenotyping. However, RSA has several drawbacks: it relatively low throughput, high variance due to microscopy readout, correlates poorly with benchmark vivo resistance, patient clearance half-life post-artemisinin treatment. Here a modified presented, extended Recovery Ring-stage...
Abstract Background The cyclical nature of parasite gene expression in the intraerythrocytic development cycle (IDC) human blood confounds accurate detection specific transcriptional differences due to drug resistance Plasmodium falciparum . Here, we propose use regression covariates eliminate major confounding developmentally driven changes with response. We show that elimination this can reduce both Type I and II errors, demonstrate effect approach on real data. Results apply method two...
Introduction: Hypotension related to autonomic dysfunction presents a particular challenge in patients on vasopressors who are refractory conventional measures. We describe case of where utilization pseudoephedrine successfully increased blood pressure (BP) and heart rate (HR) aided discontinuation vasopressors. Description: A 46-year-old male with history chronic intranasal cocaine use C5-C7 incomplete quadriplegia complicated by neurogenic orthostatic hypotension (on outpatient midodrine)...