A5103383699- Cell death mechanisms and regulation
- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Cancer-related Molecular Pathways
- Retinoids in leukemia and cellular processes
- Biochemical and biochemical processes
- NF-κB Signaling Pathways
- Peptidase Inhibition and Analysis
- Autophagy in Disease and Therapy
- Parkinson's Disease Mechanisms and Treatments
- RNA Interference and Gene Delivery
- Market Dynamics and Volatility
- Energy, Environment, and Transportation Policies
- CRISPR and Genetic Engineering
- Computational Drug Discovery Methods
- Immunotherapy and Immune Responses
- Financial Risk and Volatility Modeling
- Blood Coagulation and Thrombosis Mechanisms
- interferon and immune responses
- HIV/AIDS drug development and treatment
The University of Texas MD Anderson Cancer Center
2025
Research Network (United States)
2025
Research Manitoba
1995-1998
University of Manitoba
1992-1998
Massachusetts General Hospital
1998
Harvard University
1998
University of Toronto
1998
University of Maryland, Baltimore
1998
Temple University
1998
Boston Children's Hospital
1998
Louise Bergeron, Gloria I. Perez, Glen Macdonald, Lianfa Shi, Yi Sun, Andrea Jurisicova, Sue Varmuza, Keith E. Latham, Jodi A. Flaws, Jessica C.M. Salter, Hideaki Hara, Michael Moskowitz, En Li, Arnold Greenberg, Jonathan L. Tilly, and Junying Yuan Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115 USA; The Vincent Center for Reproductive Obstetrics Gynecology, General Hospital/Harvard 02114 Manitoba Institute Cancer Treatment Research Foundation, University...
We recently reported the purification of a lymphocyte granule protein called "fragmentin," which was identified as serine protease with ability to induce oligonucleosomal DNA fragmentation and apoptosis (Shi, L., R. P. Kraut, Aebersold, A. H. Greenberg. 1992. J. Exp. Med. 175:553). have now purified two additional proteases fragmentin activity from granules. The three are types; one has unusual cleave tripeptide thiobenzyl ester substrate after aspartic acid, similar murine cytotoxic cell...
We report the purification from a rat natural killer (RNK) large granular lymphocyte leukemia of 32-kD granule protein that induces rapid DNA fragmentation and apoptosis. The protein, which we have called "fragmentin," was capable causing intact YAC-1 cells to be cleaved into oligonucleosomal-sized fragments producing severe chromatin condensation within 1 h. Amino acid sequence tryptic peptides indicated fragmentin highly homologous NK T cell serine proteases RNK protease mouse cytotoxic I...
Cytotoxic T lymphocytes (CTL) can induce apoptosis through a granzyme B-based killing mechanism. Here we show that in cells undergoing by B, both p45 pro-interleukin 1 beta converting enzyme (ICE) and pro-CPP32 are processed. Using ICE deficient (ICE -/-) mice, embryonic fibroblasts exhibit high levels of resistance to B or 3, while lymphoblasts B-resistant, thus identifying an ICE-dependent apoptotic pathway is activated CTL granzymes. In contrast, alternative ICE-independent must also be...
Granzymes are a family of granule-associated serine esterases that mediate apoptosis by cytotoxic T lymphocytes and natural killer cells. We have previously shown cdc2, the mitosis-regulating cyclin-dependent kinase, is required for granzyme B-induced in target In addition, B induces premature activation tyrosine dephosphorylation cdc2 during apoptosis. Throughout most cell cycle until prepared to enter mitosis, kinase activity negatively regulated phosphorylation residue within its...
Background and Objectives: Mutations in the Leucine-rich repeat kinase 2 (LRRK2) gene are one of most common genetic causes Parkinson's disease (PD) linked to immune dysregulation both central nervous system periphery. However, peripheral profiles soluble factors associated with LRRK2 mutations PD have not been comprehensively characterized. Using serum CSF samples from Cohort Consortium (LCC), this study aimed probe a broad range biomarkers PD. Methods: We investigated levels regulators...
Granzyme B rapidly induces apoptosis in the presence of pore-forming protein perforin. We have examined cell cycle restriction this by separating Jurkat cells into fractions representing different stages centrifugal elutriation. Cells were susceptible to from G1 through G2/M, with no significant resistance detected at any stage. Similarly, arrested G1/S or G2/M either hydroxyurea nocodazole slightly more sensitive than asynchronously growing cells. Cdc2 kinase activity and requires its...