A5091131526- Biochemical and Molecular Research
- Enzyme Structure and Function
- Neonatal Health and Biochemistry
- ATP Synthase and ATPases Research
- Adenosine and Purinergic Signaling
- Cancer Research and Treatments
- Protein Structure and Dynamics
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Metabolic Engineering and Bioproduction
- Cancer-related molecular mechanisms research
- Machine Learning in Bioinformatics
- Plant biochemistry and biosynthesis
- Advanced Proteomics Techniques and Applications
- Bacillus and Francisella bacterial research
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Microbial Metabolism and Applications
- Genetic and phenotypic traits in livestock
- SARS-CoV-2 and COVID-19 Research
- Bioinformatics and Genomic Networks
- Microbial Inactivation Methods
- Parasitic Infections and Diagnostics
Guangxi Buffalo Research Institute
2025
Dalhousie University
2024
Argonne National Laboratory
2002-2013
Abstract The Bacillus subtilis KinD signal‐transducing histidine kinase is a part of the sporulation phosphorelay known to regulate important developmental decisions such as and biofilm formation. We have determined crystal structures extracytoplasmic sensing domain KinD, which was copurified crystallized with pyruvate ligand. structure ligand‐binding site mutant also determined; it an acetate segment similar that several other domains signal transduction proteins composed tandem...
Asian water buffaloes (Bubalus bubalis) in the Yangtze River Basin of China are important meat provider for local residents because its outstanding body size. Several previous studies have highlighted their genetic basis growth characteristics, but crucial genes regulating traits via multi-layer omics still rarely investigated. We conducted a comprehensive multi-omics analysis integrating blood and muscle transcriptome, plasma metabolome, rumen fluid metagenome, genome Haizi buffaloes. Of...
High-throughput approaches for gene cloning and expression require the development of new nonstandard tools molecular biologists biochemists. We introduce a Web-based tool to design primers specifically generation clones both laboratory-scale high-throughput projects. The application is designed not only allow user complete flexibility specify primer parameters but also minimize amount manual intervention needed generate large number simultaneous amplification multiple target genes.
Abstract Asp residues are significantly under represented in β‐sheet regions of proteins, especially the middle β‐strands, as found by a number studies using statistical, modeling, or experimental methods. To further understand reasons for this representation Asp, we prepared and analyzed mutants β‐domain. Two Gln immunoglobulin light‐chain variable domain (V L ) protein Len were replaced with then effects these changes on stability structure studied. The replacement Q38D, located at end...
The automation of protein structure determination is an essential component for high-throughput structural analysis in X-ray crystallography and a key element genomics. This highly challenging undertaking relies at present on the availability high-quality native derivatized crystals diffracting to high or moderate resolution, respectively. Obtaining such often requires significant effort. study demonstrates that phases obtained low resolution (>3.0 Å) from SeMet-labeled can be successfully...