A5017113385- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Glycosylation and Glycoproteins Research
- Transgenic Plants and Applications
- Biofuel production and bioconversion
University of Birmingham
2022-2024
University of Colorado Boulder
2022
Abstract Bacterial cell growth and division require the coordinated action of enzymes that synthesize degrade wall polymers. Here, we identify cleave d -arabinan core arabinogalactan, an unusual component Mycobacterium tuberculosis other mycobacteria. We screened 14 human gut-derived Bacteroidetes for arabinogalactan-degrading activities identified four families glycoside hydrolases with activity against or -galactan components arabinogalactan. Using one these isolates exo-...
Mycobacterial glycolipids are important cell envelope structures that drive host-pathogen interactions. Arguably, the most lipoarabinomannan (LAM) and its precursor, lipomannan (LM), which trafficked from bacterium to host via unknown mechanisms. Arabinomannan is thought be a capsular derivative of these molecules, lacking lipid anchor. However, mechanism by this material generated has yet elucidated. Here, we describe identification glycoside hydrolase family 76 enzyme term LamH (Rv0365c in...
The major nutrients available to the human colonic microbiota are complex glycans derived from diet. To degrade this highly variable mix of sugar structures, gut microbes have acquired a huge array different carbohydrate-active enzymes (CAZymes), predominantly glycoside hydrolases, many which specificities that can be exploited for range applications. Plant N -glycans prevalent on proteins produced by plants and thus components diet, but breakdown these molecules has not been explored. also...
Abstract Mycobacterial glycolipids are important cell envelope structures that drive host-pathogen interactions. Arguably, the most amongst these lipoarabinomannan (LAM) and its precursor, lipomannan (LM), which both trafficked out of bacterium to host via unknown mechanisms. An class exported LM/LAM is capsular derivative molecules devoid lipid anchor. Here, we describe identification a glycoside hydrolase family 76 enzyme term LamH specifically cleaves α-1,6-mannoside linkages within LM...
Abstract The major nutrients available to the human colonic microbiota are complex glycans derived from diet. To degrade this highly variable mix of sugar structures, gut microbes have acquired a huge array different carbohydrate-active enzymes (CAZymes), predominantly glycoside hydrolases, many which specificities that can be exploited for range applications. Plant N-glycans prevalent on proteins produced by plants and thus components diet, but breakdown these molecules has not been...