A5016480427- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Sarcoidosis and Beryllium Toxicity Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Systemic Lupus Erythematosus Research
- Immune Response and Inflammation
- Total Knee Arthroplasty Outcomes
- Immune Cell Function and Interaction
- Orthopaedic implants and arthroplasty
- Monoclonal and Polyclonal Antibodies Research
- Hematopoietic Stem Cell Transplantation
- Immunodeficiency and Autoimmune Disorders
- Orthopedic Infections and Treatments
- Trypanosoma species research and implications
- Osteoarthritis Treatment and Mechanisms
- Multiple Myeloma Research and Treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Rheumatoid Arthritis Research and Therapies
- Chemokine receptors and signaling
- Systemic Sclerosis and Related Diseases
- Cancer Genomics and Diagnostics
- Chronic Myeloid Leukemia Treatments
- Advanced Breast Cancer Therapies
- Reproductive System and Pregnancy
- S100 Proteins and Annexins
University Hospital Olomouc
2011-2025
Palacký University Olomouc
2016-2025
Czech Academy of Sciences, Biology Centre
2009-2021
Institute of Parasitology
2008-2021
Institute of Molecular and Translational Medicine
2010-2013
University of South Bohemia in České Budějovice
2008-2009
Czech Academy of Sciences
2008-2009
Royal Brompton Hospital
2006
Friedrich Schiller University Jena
2006
The insufficient standardisation of diagnostic next-generation sequencing (NGS) still limits its implementation in clinical practice, with the correct detection mutations at low variant allele frequencies (VAF) facing particular challenges. We address here coverage depth order to minimise probability false positive and negative results, latter being underestimated NGS. There is currently no consensus on minimum depth, so each laboratory has set own parameters. To assist laboratories...
Pirfenidone, an antifibrotic drug, slows-down the disease progression in idiopathic pulmonary fibrosis (IPF) over 12 months, however limited data on decline of lung function and overall survival (OS) real-world cohorts longer follow-up exists. Of enrolled Czech IPF patients (n = 841) from EMPIRE registry, 383 (45.5%) received pirfenidone, 218 (25.9%) no-antifibrotic treatment 240 (28.5%) were excluded (missing data, nintedanib treatment). The 2- 5-yrs OS forced vital capacity (FVC) diffusing...
In vitro cytotoxicity analysis of doxorubicin-loaded/superparamagnetic iron oxide colloidal nanoassemblies on MCF7 and NIH3T3 cell lines Katerina Tomankova,1 Polakova,2 Klara Pizova,1 Svatopluk Binder,1 Marketa Havrdova,2 Mary Kolarova,2 Eva Kriegova,3 Jana Zapletalova,1 Lukas Malina,1 Horakova,1 Jakub Malohlava,1 Argiris Kolokithas-Ntoukas,4 Aristides Bakandritsos,4 Hana Kolarova,1 Radek Zboril2 1Department Medical Biophysics, Institute Translation Medicine, Faculty Medicine Dentistry,...
Inflammation, an essential tissue response to extrinsic/intrinsic damage, is a very dynamic process in terms of complexity and extension cellular metabolic involvement. The aim the inflammatory eliminate pathogenic initiator with limited collateral damage inflamed tissue, followed by complex repair preinflammation phenotype. Persistent inflammation major contributor pathogenesis many musculoskeletal diseases including ageing-related pathologies such as osteoporosis, osteoarthritis,...
Matrix metalloproteinases (MMP) and tissue inhibitors of (TIMP) have been implicated in chronic obstructive pulmonary disease (COPD) pathogenesis. However, the majority studies focused on single MMP, there is limited information parallel expression MMP their antagonists TIMP. We, therefore, investigated serum profile 1-3, 7-9, 12 13, TIMP 1-4 COPD patients.Serum were measured 74 patients 20 control subjects by multiple microsphere technology.MMP 1-3 7-9 elevated compared with (P=...
Due to the lack of protective immunity in general population and absence effective antivirals vaccines, Coronavirus disease 2019 (COVID-19) pandemic continues some countries, with local epicentres emerging others. great demand for COVID-19 testing programmes control spread disease, we have suggested such a programme that includes rapid RT-qPCR approach without RNA extraction. The Direct-One-Step-RT-qPCR (DIOS-RT-qPCR) assay detects severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)...
Abstract Extramedullary disease (EMM) represents a rare, aggressive and mostly resistant phenotype of multiple myeloma (MM). EMM is frequently associated with high-risk cytogenetics, but their complex genomic architecture largely unexplored. We used whole-genome optical mapping (Saphyr, Bionano Genomics) to analyse the CD138+ cells isolated from bone-marrow aspirates an unselected cohort newly diagnosed patients (n = 4) intramedullary MM 7). Large intrachromosomal rearrangements (> 5 Mbp)...
Summary Trypanosoma brucei use microsomal elongases for de novo synthesis of most its fatty acids. In addition, this parasite utilizes an essential mitochondrial type II synthase production octanoate (a lipoic acid precursor) as well longer acids such palmitate. Evidence from other organisms suggests that mitochondrially synthesized are required efficient respiration but the exact relationship remains unclear. procyclic form trypanosomes, we also found RNAi depletion acyl carrier protein,...
Systemic lupus erythematosus (SLE) is a remarkably heterogeneous autoimmune disease. Despite tremendous efforts, our knowledge of serum protein patterns in severe SLE phenotypes still limited. We investigated the pattern SLE, with special emphasis on irreversible organ damage and active nephritis (LN) as assessed by renal Lupus Erythematosus Disease Activity Index. used proximity extension immunoassay (PEA, Proseek Multiplex, Olink) to assess levels ninety-two inflammation-related proteins...
Mantle cell lymphoma (MCL) is a subtype of B-cell with large number recurrent cytogenetic/molecular aberrations. Approximately 5–10% patients do not respond to frontline immunochemotherapy. Despite many useful prognostic indexes, reliable marker chemoresistance available. We evaluated the impact seven gene aberrations including tumor suppressor protein P53 (TP53) and cyclin dependent kinase inhibitor 2A (CDKN2A) in cohort 126 newly diagnosed consecutive MCL bone marrow involvement ≥5% using...
N-acetylcysteine (NAC), often used as an antioxidant-scavenging reactive oxygen species (ROS) in vitro, was recently shown to increase the cytotoxicity of other compounds through ROS-dependent and ROS-independent mechanisms. In this study, NAC itself found induce extensive ROS production human leukemia HL-60 U937 cells. The depends on ROS-modulating enzyme expression. cells, activated NOX2 produce superoxide (O2•-). Its subsequent conversion into H2O2 by dismutase 1 3 (SOD1, SOD3) ClO- from...
For accuracy of quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), normalisation with suitable reference genes is required. To date, no have been validated for expression studies bronchoalveolar (BAL) cells. The aims this study were to identify gene(s) stable mRNA in BAL cells irrespective gender, smoking, cellular composition, lung pathology, treatment; and assess the influence on target gene data. ten housekeeping (ACTB, ARF1, CANX, G6PD, GAPDH, GPS1, GNB2L1, PSMB2,...
A growing body of studies highlights involvement neutrophils in cancer development and progression. Our aim was to assess the phenotypic functional properties circulating from patients with chronic lymphocytic leukemia (CLL). The percentage CD54+ CD64+ as well CD54 expression on these cells were higher CLL than age-matched healthy controls. Neutrophils produced more reactive oxygen species (ROS) compared controls both resting activated conditions. Lipopolysaccharide-induced production IL-1β...
To determine differences in the blood innate gene expression signatures of systemic lupus erythematosus (SLE) patients across various organ manifestations and disease activity, with a focus on nephritis (LN) central nervous system (CNS) involvement. Toll-like receptor family (TLR 1–10) mRNA was investigated peripheral mononuclear cells from SLE (n = 74) healthy controls 34). We compared histologically confirmed active LN or neuropsychiatric (NPSLE) without these symptoms. The TLR determined...