A5075480521- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Structural Characterization
- Protein purification and stability
- Advanced Biosensing Techniques and Applications
- Protein Structure and Dynamics
- Erythrocyte Function and Pathophysiology
- Glycosylation and Glycoproteins Research
- Alzheimer's disease research and treatments
- Hepatitis C virus research
- Protein Tyrosine Phosphatases
- Computational Drug Discovery Methods
- Chemical Synthesis and Analysis
- Genetics, Bioinformatics, and Biomedical Research
- Enzyme Structure and Function
Granta Design (United Kingdom)
2020-2023
University of York
2020-2023
HUN-REN Research Centre for Natural Sciences
2022
Surface plasmon resonance (SPR) biosensor methods are ideally suited for fragment-based lead discovery. However, generally applicable experimental procedures and detailed protocols lacking, especially structurally or physico-chemically challenging targets when tool compounds not available. Success depends on accounting the features of both target chemical library, purposely designing screening experiments identification validation hits with desired specificity mode-of-action, availability...
Intrinsically disordered proteins (IDPs) play important roles in disease pathologies; however, their lack of defined stable 3D structures make traditional drug design strategies typically less effective against these targets. Based on promising results targeted covalent inhibitors (TCIs) challenging targets, we have developed a strategy targeting IDPs. As model system chose tau, an endogenous IDP the central nervous that is associated with severe neurodegenerative diseases via its...
Over the past decade intrinsically disordered proteins (IDPs) have emerged as a biologically important class of proteins, many which are therapeutic relevance. Here, we investigated interactions between model IDP system, tau K18, and nine literature compounds that been reported having an effect on in order to identify robust IDP–ligand system for optimization range biophysical methods. We used NMR, surface plasmon resonance (SPR) microscale thermophoresis (MST) methods investigate binding...
Abstract Surface plasmon resonance biosensor technology (SPR) is ideally suited for fragment-based lead discovery. However, generally suitable experimental procedures or detailed protocols are lacking, especially structurally physico-chemically challenging targets when tool compounds lacking. Success depends on accounting the features of both target and chemical library, purposely designing screening experiments identification validation hits with desired specificity mode-of-action,...