Humberto Prieto

ORCID: 0000-0002-9013-1906
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About
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Research Areas
  • Plant Virus Research Studies
  • Plant Reproductive Biology
  • Plant tissue culture and regeneration
  • Horticultural and Viticultural Research
  • Plant and Fungal Interactions Research
  • Plant Pathogens and Fungal Diseases
  • Plant Molecular Biology Research
  • Plant Physiology and Cultivation Studies
  • CRISPR and Genetic Engineering
  • Plant-Microbe Interactions and Immunity
  • Plant Gene Expression Analysis
  • Postharvest Quality and Shelf Life Management
  • Photosynthetic Processes and Mechanisms
  • Transgenic Plants and Applications
  • Fermentation and Sensory Analysis
  • Plant pathogens and resistance mechanisms
  • Plant Pathogenic Bacteria Studies
  • Phytoplasmas and Hemiptera pathogens
  • Animal Genetics and Reproduction
  • Plant biochemistry and biosynthesis
  • Berry genetics and cultivation research
  • Genetically Modified Organisms Research
  • Polysaccharides and Plant Cell Walls
  • Phytochemicals and Antioxidant Activities
  • RNA Interference and Gene Delivery

Instituto de Investigaciones Agropecuarias
2015-2025

Inria Chile
2024

Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2005-2019

Biotechnology Research Center
2014

Universidad Bernardo O'Higgins
2007

Universidad de Santiago de Chile
2007

University of La Serena
2007

University of Chile
1995-1998

Summary A special regulatory regime applies to products of recombinant nucleic acid modifications. ruling from the European Court Justice has interpreted this in a way that it also emerging mutagenesis techniques. Elsewhere progress is ongoing. In 2015, Argentina launched framework, followed by Chile 2017 and recently Brazil Colombia. March 2018, USDA announced will not regulate genome‐edited plants differently if they could have been developed through traditional breeding. Canada an...

10.1111/nph.15627 article EN New Phytologist 2018-12-13

Epigenetic modifications can yield information about connections between genotype, phenotype variation and environmental conditions. Bud dormancy release in temperate perennial fruit trees depends on internal signals such as cold accumulation photoperiod. Previous investigations have noted the participation of epigenetic mechanisms control this physiological process. We examined whether were modulated MADS-box genes, potential candidates for regulation bud flowering sweet cherry (Prunus...

10.1093/treephys/tpx055 article EN Tree Physiology 2017-05-11

Recently, the plant hormone abscisic acid has been involved as key player in regulation of endodormancy grapevine buds (Vitis vinifera L). In this study, we show that vine, expression genes related to biosynthesis ABA (VvNCED1; VvNCED2) and content are significantly higher at latent bud than shoot-apex, while catabolic gene (VvA8H3) showed no significant differences between both organs. A negative correlation transcript levels cell cycle (CCG) was found tissues. This result, suggested may...

10.3389/fpls.2017.00812 article EN cc-by Frontiers in Plant Science 2017-05-18

Genetic engineering based on Agrobacterium-mediated transformation has been a desirable tool to manipulate single or multiple genes of existing genotypes woody fruit crops, for which conventional breeding is difficult and lengthy process due heterozygosity, sexual incompatibility, juvenility, lack natural sources. To date, successful reported many crops. We review the major progress in genetic these crops made past 5 years, emphasizing reproducible protocols as well strategies that have...

10.3389/fpls.2019.00226 article EN cc-by Frontiers in Plant Science 2019-02-28

Due to the low incidence of precipitation attributed climate change, many high-altitude Andean lakes (HAALs) and lagoons distributed along central Andes in South America may soon disappear. This includes La Brava-La Punta, a brackish lake system located south Salar de Atacama within hyper-arid halophytic biome Desert. Variations physicochemical parameters water column can induce changes microbial community composition, which we aimed determine. Sixteen sampling points across Punta were...

10.3390/microorganisms8081181 article EN cc-by Microorganisms 2020-08-03

The woody nature of grapevine ( Vitis vinifera L.) has hindered the development efficient gene editing strategies to improve this species. lack highly transfer techniques, which, furthermore, are applied in multicellular explants such as somatic embryos, additional technical handicaps vine. inclusion geminivirus-based replicons regular T-DNA vectors can enhance expression clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) elements, thus...

10.3389/fpls.2021.791030 article EN cc-by Frontiers in Plant Science 2021-12-23

Trypanosoma cruzi and Toxoplasma gondii are two parasites than can be transmitted from mother to child through the placenta. However, congenital transmission rates low for T. high gondii. Infection success or failure depends on complex parasite-host interactions in which alter host gene expression by modulating non-coding RNAs such as miRNAs. As of yet, there no reports altered miRNA placental tissue response either parasite. Therefore, we infected human explants ex vivo cultivation with 2...

10.3389/fimmu.2020.595250 article EN cc-by Frontiers in Immunology 2020-11-06

The flowering and fruiting of sweet cherry (Prunus avium L.) depend on precise synchronization with seasonal events. During harsh autumn winter conditions, floral buds enter dormancy to protect prepare for the productive season. Dormancy release occurs after exposure genotype-specific chilling temperatures, an event in which epigenetic reprogramming triggers further metabolic gene expression activation. Similarly, several Arabidopsis ecotypes require (vernalization) transition from...

10.3390/plants14060899 article EN cc-by Plants 2025-03-13

Postharvest softening of grape berries is one the main problems affecting quality during export. Cell wall disassembly, especially pectin polysaccharides, has been commonly related to fruit softening, but its influence poorly studied in grapes postharvest life. In order better understand this process, Thompson seedless (TS) variety, which significantly decreased berry texture after prolonged cold storage, was compared NN107, a new table variety with higher firmness. Biochemical analysis...

10.3390/molecules20033667 article EN cc-by Molecules 2015-02-23

Cherimoya (Annona cherimola Mill.) fruit is an attractive candidate for food processing applications as fresh cut. However, along with its desirable delicate taste, cherimoya shows a marked susceptibility to browning. This condition mainly attributed polyphenol oxidase activity (PPO). A general lack of knowledge regarding PPO and role in the oxidative loss quality processed requires better understanding mechanisms involved. The work carried out included cloning full-length cDNA, analysis...

10.1021/jf070586+ article EN Journal of Agricultural and Food Chemistry 2007-10-01

A new plant commensal Pseudomonas veronii isolate (strain R4) was identified from a Xiphinema index biocontrol screen. Isolated grapevine roots vineyards in central Chile, the strain R4 exhibited slower yet equivalently effective nematicide activity as well-characterized P. protegens CHA0. Whole genome sequencing of and comparative analysis among available spp. genomes allowed for identification gene clusters that encode putative extracellular proteases lipase synthesis secretion systems,...

10.1186/s40793-016-0198-y article EN cc-by Standards in Genomic Sciences 2016-10-11

In plants, host factors encoded by susceptibility (S) genes are indispensable for viral infection. Resistance is achieved through the impairment or absence of those factors. Many S have been cloned from model and crop species a majority them coding members eukaryotic translation initiation complex, mainly eIF4E, eIF4G their isoforms. The aim this study was to investigate role in stone fruit sharka, disease due Plum pox virus (PPV).For purpose, hairpin-inducing silencing constructs based on...

10.1186/s12870-019-2047-9 article EN cc-by BMC Plant Biology 2019-10-22
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