A5087693700- Analytical Chemistry and Chromatography
- Drug Transport and Resistance Mechanisms
- Cell death mechanisms and regulation
- Viral-associated cancers and disorders
- Protein purification and stability
- Cancer-related Molecular Pathways
- Protease and Inhibitor Mechanisms
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Peptidase Inhibition and Analysis
- Blood properties and coagulation
- Cancer Mechanisms and Therapy
- Microtubule and mitosis dynamics
- Metabolomics and Mass Spectrometry Studies
- RNA Interference and Gene Delivery
- Chromatography in Natural Products
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Histiocytic Disorders and Treatments
- Melanoma and MAPK Pathways
- Microfluidic and Capillary Electrophoresis Applications
Sanyo-Onoda City University
2025
Tokyo University of Science
2020-2024
National Institute of Infectious Diseases
2001-2024
Target (United States)
2023-2024
Keio University
2012-2022
National Institutes of Health
2004-2006
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2006
Laboratoire de Chémo-biologie synthétique et thérapeutique
2004
National Cancer Research Institute
1997-1999
The University of Tokyo
1967-1997
The pim-1 oncogene encodes a serine/threonine kinase (Pim-1) involved in the transduction of cytokine-triggered mitogenic signals. Pim-1 is unique that it closely cooperates with c-Myc not only oncogenesis, but also apoptosis induction. However, molecular basis function remains poorly understood, largely because downstream effector molecule(s) for has been identified. Here we provide several lines evidence Cdc25A cell cycle phosphatase, direct transcriptional target c-Myc, substrate and...
Multidrug resistance (MDR) in cancer cells is a phenotype whereby display reduced sensitivity to anticancer drugs, based on variety of mechanisms, including an increase drug efflux, the reduction uptake, activation cell growth and survival signaling, promotion DNA repair, inhibition apoptosis signaling. Increased expression plasma membrane efflux pumps, ATP-binding cassette (ABC) transporters, involved MDR. P-Glycoprotein/ABCB1 member ABC transporter family, facilitates various...
The expression of c-<i>myc</i> promotes cell proliferation and also sensitizes cells to various extracellular apoptotic stimuli. However, signal pathways regulating the function Myc proteins during apoptosis are unknown. c-Jun N-terminal kinase (JNK) is activated by stimuli, but neither target molecule(s) or action JNK has been identified in Myc-mediated apoptosis. Here, we found that selectively interacted with, phosphorylated, c-Myc at Ser-62 Ser-71 as confirmed with phospho-c-Myc-specific...
Gel‐exclusion high‐performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on PGP 2000 column (0.10 M sodium phosphate buffer, 0.30 NaCl, pH 6.86) showed at least two peaks, the principal component corresponding to mercaptalbumin (HMA) and second one nonmercaptalbumin (HNA). Mechanism for separation HMA HNA might be due weak resin‐HSA interaction. HPLC bovine plasma (BPA) a single peak column. The elution volume HSA was larger than that BPA, resulting in clear resolution BPA.
Expression of P-glycoprotein (P-gp)/ABCB1 on cancer cell surfaces is a critical determinant anticancer drug resistance. Regulators P-gp expression and function are key molecules controlling Here we report the mechanism underlying ubiquitin-proteasome pathway-mediated degradation P-gp. The proteasome inhibitor MG132 increased level, enhanced its ubiquitination, delayed disappearance ubiquitinated To search for regulators MALDI-time flight mass spectrometry analyses were carried out, 22...
DNA replication and genotoxic stresses activate various checkpoint-associated protein kinases, checkpoint dysfunction often leads to cell lethality. Here, we have identified new members of the mammalian NIMA family termed Nek11L Nek11S (NIMA-related kinase11Long Short isoform) as novel replication/damage stresses-responsive kinases. Molecular cloning biochemical studies showed that catalytic domain Nek11 is most similar Nek4 Nek3, substrate specificity distinguishable from those Nek2. The...
Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) inhibit the function of certain adenosine triphosphate (ATP)-binding cassette transporters, including P-glycoprotein/ABCB1 and breast cancer resistance protein (BCRP)/ABCG2. We previously reported an antagonistic activity gefitinib towards BCRP. have now analyzed effects erlotinib, another EGFR-TKI, on P-glycoprotein As with gefitinib, erlotinib effectively reversed BCRP-mediated to SN-38 (7-ethyl-10-hydroxycamptothecin)...
Highlights•Anti-SARS-CoV-2 bispecific Abs can be generated by combining non-neutralizing Abs•Anti-S2 antibody CvMab-62 recognizes a novel epitope upstream of S2 stem helix•Bispecific antibodies inhibit spike-mediated membrane fusogenic mechanism•Bispecific restore antiviral activity against bebtelovimab-resistant BQ.1.1SummaryA current challenge is the emergence SARS-CoV-2 variants, such as BQ.1.1 and XBB.1.5, that evade immune defenses, thereby limiting drug effectiveness. Emergency-use...
Upon activation, cell surface death receptors, Fas/APO-1/CD95 and tumor necrosis factor receptor-1 (TNFR-1), are attached to cytosolic adaptor proteins, which in turn recruit caspase-8 (MACH/FLICE/Mch5) activate the interleukin-1 β-converting enzyme (ICE)/CED-3 family protease (caspase) cascade. However, it remains unknown whether these apoptotic proteases generally involved apoptosis triggered by other stimuli such as Myc p53. In this study, we provide lines of evidence that a cascade...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSlalom chromatography: size-dependent separation of DNA molecules by a hydrodynamic phenomenonJun Hirabayashi, Naofumi Ito, Kohji Noguchi, and Kenichi KasaiCite this: Biochemistry 1990, 29, 41, 9515–9521Publication Date (Print):October 1, 1990Publication History Published online1 May 2002Published inissue 1 October 1990https://doi.org/10.1021/bi00493a004RIGHTS & PERMISSIONSArticle Views335Altmetric-Citations43LEARN ABOUT THESE METRICSArticle Views...
Vascular endothelial growth factor (VEGF) and its receptors are highly expressed in Kaposi's sarcoma (KS) lesion play a key role angiogenesis. Latency-associated nuclear antigen (LANA) of sarcoma-associated herpesvirus (KSHV/HHV8) has multiple functions related to viral latency KSHV-induced oncogenesis. In this report, we have identified Daxx as LANA-binding protein by co-immunoprecipitation analysis HeLa cells stably expressing LANA. LANA associated with PEL cell line infected KSHV. also...
Abstract P-glycoprotein (P-gp) is a critical determinant of multidrug resistance in cancer. We previously reported that MAPK inhibition downregulates P-gp expression and undergoes ubiquitin–proteasomal degradation regulated by UBE2R1 SCF Fbx15 . Here, we investigated the crosstalk between P-gp. Proteasome inhibitors or knockdown FBXO15 and/or cancelled MEK inhibitor-induced downregulation. RSK1 phosphorylated Thr162 on but did not phosphorylate FBXO15. RSK increased UBE2R1-WT UBE2R1-T162D...