A5078187729- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Biofuel production and bioconversion
- Microbial Fuel Cells and Bioremediation
- Microbial Metabolic Engineering and Bioproduction
- RNA Research and Splicing
- HIV/AIDS drug development and treatment
- Hepatitis C virus research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Advanced biosensing and bioanalysis techniques
- Carbohydrate Chemistry and Synthesis
- Electrochemical sensors and biosensors
- Biochemical and Molecular Research
- Hepatitis B Virus Studies
- Chemical Synthesis and Analysis
- Synthesis of Organic Compounds
- Microbial Metabolites in Food Biotechnology
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Spectroscopy and Chemometric Analyses
- Bacterial biofilms and quorum sensing
- Supercapacitor Materials and Fabrication
- Crystallography and molecular interactions
- Lanthanide and Transition Metal Complexes
DEVCOM Army Research Laboratory
2012-2025
United States Army Combat Capabilities Development Command
2021-2025
Medivir (Sweden)
2004-2017
Roche (Sweden)
2008-2009
Bipar
2007-2008
Uppsala University
1989-2007
La Roche College
2007
Roche (United States)
2007
East Carolina University
2006
Universität Hamburg
2001-2002
Transcriptional analysis was performed on Clostridium acetobutylicum with the goal of identifying sugar-specific mechanisms for transcriptional regulation transport and metabolism genes. DNA microarrays were used to determine transcript levels from total RNA isolated cells grown media containing eleven different carbohydrates, including two pentoses (xylose, arabinose), four hexoses (glucose, mannose, galactose, fructose), disaccharides (sucrose, lactose, maltose, cellobiose) one...
Numerous bacterial genera are known to respire anaerobically using macroscopic electrodes as electron acceptors. Typically, inexpensive graphite electrodes, which readily colonized, used monitor electrogenic metabolism for microbial fuel cell and bioelectronics studies. We compare current production by bacteria on gold coated with various alkanethiol self-assembled monolayers glassy carbon electrodes. Current is correlated chain length headgroup of the monolayer molecules expected. Relative...
Summary The intestinal anaerobic symbiont, Bacteroides fragilis , is highly aerotolerant and resistant to H 2 O . Analysis of the transcriptome showed that expression 45% genome was significantly affected by oxidative stress. gene patterns suggested exposure stress induced an acute response rapidly minimize immediate effects reactive oxygen species, then upon extended a broad metabolic induced. This genes encoding enzymes can supply reducing power for detoxification restore energy‐generating...
Abstract Twelve heteroaromatic complexing agents 9a–I were synthesized with the purpose to develop suitable labels for time‐resolved luminescence‐based bioaffinity assays. The relative luminescence yields, excitation maxima, and emission decay constants of their europium(III) terbium(III) chelates determined. According these results, 2,2′,2″,2‴‐[(2,2′‐bipyridine‐6,6′‐diyl)bis(methylenenitrilo)]tetrakis (acetic acid) ( 9e ) 2,2′,2″,2‴‐[(2,2′:6′,2″‐terpyridine‐6,6″‐diyl)bis(methylenenitrilo)]...
The discovery of 4′-azidocytidine (3) (R1479) (J. Biol. Chem. 2006, 281, 3793; Bioorg. Med. Lett. 2007, 17, 2570) as a potent inhibitor RNA synthesis by NS5B (EC50 = 1.28 μM), the polymerase encoded hepatitis C virus (HCV), has led to and biological evaluation several monofluoro difluoro derivatives 4′-azidocytidine. most compounds in this series were 4′-azido-2′-deoxy-2′,2′-difluorocytidine 4′-azido-2′-deoxy-2′-fluoroarabinocytidine with antiviral EC50 66 nM 24 HCV replicon system,...
An amphiphilic conjugated oligoelectrolyte (COE) that spontaneously intercalates into lipid membranes enables Shewanella oneidensis to use a graphite electrode as the sole electron donor for succinate production. Current consumed in poised electrochemical system by with micromolar concentrations of COE correlates well produced via fumarate reduction determined HPLC analysis. Confocal microscopy confirms incorporation microbes on surface. This work presents unique strategy induce favorable...
The current therapy for hepatitis C virus (HCV) infection has limited efficacy, in particular against the genotype 1 virus, and a range of side effects. In this context high unmet medical need, more efficacious drugs targeting HCV nonstructural proteins are interest. Here we describe 2'-deoxy-2'-spirocyclopropylcytidine (5) as new inhibitor NS5B RNA-dependent RNA polymerase, displaying an EC(50) 7.3 μM measured Huh7-Rep cell line no associated cytotoxicity (CC(50) > 98.4 μM). Computational...
Abstract Three different oligo DNA probes were synthesized and their value as hybridization was tested.
4'-Azidocytidine 3 (R1479) has been previously discovered as a potent and selective inhibitor of HCV replication targeting the RNA-dependent RNA polymerase hepatitis C virus, NS5B. Here we describe synthesis biological evaluation several derivatives 4'-azidocytidine by varying substituents at ribose 2' 3'-positions. The most compound in this series is 4'-azidoarabinocytidine with an IC(50) 0.17 microM genotype 1b subgenomic replicon system. structure-activity relationships within nucleoside...
Abstract 2′‐Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is a potential drug target for the treatment of malaria. We previously reported discovery 5′‐tritylated analogues deoxyuridine as selective inhibitors this Plasmodium falciparum enzyme. Herein we report further structure–activity studies; in particular, variations 5′‐trityl group, introduction various substituents at 3′‐position deoxyuridine, and modifications base. Compounds were tested against both enzyme parasite....
Clostridium acetobutylicum fermentations are promising for production of commodity chemicals from heterogeneous biomass due to the wide range substrates organism can metabolize. Much work has been done elucidate pathways utilization aldoses, but little is known about metabolism more oxidized substrates. Two hexose derivatives, gluconate and galacturonate, present in low cost feedstocks, their will contribute overall metabolic output these substrates.A complete network glucose, gluconate,...
Abstract Food waste represents an underutilized resource for commodity chemical generation. Constituents of the human gut microbiota that are already adapted to a food stream could be repurposed useful production. Industrial fermentations utilizing these microbes maintain organisms in isolation; however, microbial consortia offer attractive alternative monocultures metabolic interactions may result more efficient processes with higher yields. Here we computationally assess ability...