A5101882624- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Pancreatic and Hepatic Oncology Research
- Pancreatic function and diabetes
- Cardiovascular Conditions and Treatments
- Xenotransplantation and immune response
- Cardiac Arrhythmias and Treatments
- Animal health and immunology
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Sperm and Testicular Function
Broad Center
2023
University of California, San Francisco
2023
Shaanxi Provincial People's Hospital
2022
Shanxi Medical University
2022
Agriculture and Forestry University
2015
Northwest A&F University
2014
Numerous studies have characterized the existence of cell subtypes, along with their corresponding transcriptional profiles, within developing mouse pancreas. The upstream mechanisms that initiate and maintain gene expression programs across states, however, remain largely unknown. Here, we generate single-nucleus ATAC-Sequencing data murine pancreas perform an integrated, multi-omic analysis both chromatin accessibility RNA to describe landscape at E14.5 E17.5 single-cell resolution. We...
Abstract Objectives Ngn3 is a typical transcription factor and marker of differentiating spermatogonial stem cells ( SSC s) in mouse, belonging to the basic helix‐loop‐helix bHLH ) family. Its gene specifically expressed A type spermatogonia mouse testis, thus plays critical role controlling differentiation s. However, roles its protein dairy goat testis remain unknown. Materials methods Testis development expression patterns were analysed by immunofluorescence quantitative reverse...
Abstract Objectives Nanos2, an RNA ‐binding protein, belongs to the Nanos gene‐coding family and contains two CCHC zinc‐finger motifs. In mouse, it plays a pivotal role in male germ cell development, self‐renewal of spermatogonial stem cells. However, little is known its expression pattern functions dairy goat testis. Materials methods Immunohistochemistry quantitative reverse transcription‐polymerase chain reaction ( qRT ‐ PCR ) were used generate profile Nanos2 Furthermore, overexpression...
Available rabbit anti-NANOS2 antibodies failed to recognize the NANOS2 protein of goat origin though they showed better reactivity towards same human and mice origin. A specific antibody against is necessary explore biological functions dairy protein. Recombinant (dairy origin) was expressed in E. coli (BL21). Anti-NANOS2 immunoglobulin Y (IgY; chicken) developed evaluated. sequences between were compared using bioinformatics tools. The IgY titer attained peak (≥ 1 : 128 000) after 3rd...
心脏穿孔是起搏器置入治疗少见的并发症，起搏电极所引起的冠状动脉穿孔病例更为罕见。本文报道1例63岁老年女性，行双腔起搏器置入术后出现心脏右心耳及右冠状动脉晚期穿孔，导致心包填塞；经外科开胸对穿孔的右冠状动脉进行缝合，折叠加固部分右心耳，术后病情平稳出院；同时分析了主动起搏电极导线致右冠状动脉穿孔的可能原因，探讨了如何避免此类不良事件的发生，为今后类似病例的临床诊疗提供参考。.