A5033292178- Influenza Virus Research Studies
- Respiratory viral infections research
- Gene Regulatory Network Analysis
- Monoclonal and Polyclonal Antibodies Research
- Machine Learning in Healthcare
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Sepsis Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- interferon and immune responses
- Advanced Biosensing Techniques and Applications
- Epigenetics and DNA Methylation
- COVID-19 and healthcare impacts
- Fungal and yeast genetics research
- Viral Infections and Outbreaks Research
- Microbial Metabolic Engineering and Bioproduction
- Mathematical Biology Tumor Growth
- Photoreceptor and optogenetics research
- Artificial Intelligence in Healthcare
- COVID-19 diagnosis using AI
- vaccines and immunoinformatics approaches
- ECG Monitoring and Analysis
University of Oxford
2016-2020
Max Planck Institute for Dynamics of Complex Technical Systems
2010-2016
Max Planck Society
2010-2016
Otto-von-Guericke University Magdeburg
2015
Abstract Following DNA damage caused by exogenous sources, such as ionizing radiation, the tumour suppressor p53 mediates cell cycle arrest via expression of CDK inhibitor, p21. However, role p21 in maintaining genomic stability absence DNA-damaging agents is unclear. Here, using live single-cell measurements protein proliferating cultures, we show that naturally occurring incurred over S-phase causes p53-dependent accumulation during mother G2- and daughter G1-phases. High levels mediate G1...
Abstract Biochemical reactions are subject to stochastic fluctuations that can give rise cell-to-cell variability. Yet, how this variability affects viral infections, which themselves involve noisy reactions, remains largely elusive. Here we present single-cell experiments and simulations reveal a large heterogeneity between influenza A virus (IAV)-infected cells. In particular, experimental data show progeny titres range from 1 970 plaque-forming units intracellular RNA (vRNA) levels span...
The transition from G1 into DNA replication (S phase) is an emergent behavior resulting dynamic and complex interactions between cyclin-dependent kinases (Cdks), Cdk inhibitors (CKIs), the anaphase-promoting complex/cyclosome (APC/C). Understanding cellular decision to commit S phase requires a quantitative description of these interactions. We apply imaging single human cells track expression G1/S regulators use data parametrize stochastic mathematical model transition. show that rapid,...
Abstract Since its emergence in late 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic with more than 55 million reported cases and 1.3 estimated deaths worldwide. While epidemiological clinical characteristics of COVID-19 have been reported, risk factors underlying transition from mild to disease among patients remain poorly understood. In this retrospective study, we analysed data 879 confirmed SARS-CoV-2 positive admitted two-site NHS Trust...
Influenza viruses are a major public health burden during seasonal epidemics and continuous threat due to their potential cause pandemics. Annual vaccination provides the best protection against contagious respiratory illness caused by influenza viruses. However, current production capacities for vaccines insufficient meet increasing demands. We explored possibility establish process using duck-derived suspension cell line AGE1.CR. A two-stage bioreactor setup was designed in which cells...
Significance Controlled transitions of human cells between proliferating and nonproliferating states are essential for normal development tissue homeostasis. To understand how the decision to proliferate is made in response positive input from growth factors negative DNA damage response, we have built a mathematical model underlying molecular network, based on data live cell-imaging experiments. Our suggests that two major cell-cycle crucial making: restriction point, which integrates pro-...
Influenza viruses transcribe and replicate their negative-sense RNA genome inside the nucleus of host cells via three viral species. In course an infection, these RNAs show distinct dynamics, suggesting that differential regulation takes place. To investigate this in a systematic way, we developed mathematical model influenza virus infection at level single mammalian cell. It accounts for key steps life cycle, from entry to progeny virion release, while focusing particular on molecular...
Influenza A viruses are respiratory pathogens that cause seasonal epidemics with up to 500,000 deaths each year. Yet there currently only two classes of antivirals licensed for treatment and drug-resistant strains on the rise. major challenge discovery new anti-influenza agents is identification drug targets efficiently interfere viral replication. To support this step, we developed a multiscale model influenza virus infection which comprises both intracellular level where synthesizes its...
The size of a cell sets the scale for all biochemical processes within it, thereby affecting cellular fitness and survival. Hence, needs to be kept certain limits relatively constant over multiple generations. However, how cells measure their use this information regulate growth division remains controversial. Here, we present two mechanistic mathematical models budding yeast (S. cerevisiae) cycle investigate competing hypotheses on control: inhibitor dilution titration nuclear sites. Our...
Well-nourished cells in a favorable environment (well supplied with growth factors, cytokines, and/or hormones and free from stresses, ionizing radiation, etc.) will grow, replicate their genome, divide into two daughter cells, fully prepared to repeat the process. This cycle of DNA replication division underlies all aspects biological growth, reproduction, repair development. As such, it is essential that cell's genome be guarded against damage during replication/division process, lest...
Background Since its emergence in late 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic, with more than 4.8 million reported cases and 310 000 deaths worldwide. While epidemiological clinical characteristics of COVID-19 have been reported, risk factors underlying transition from mild to disease among patients remain poorly understood. Methods In this retrospective study, we analysed data 820 confirmed positive admitted two-site NHS Trust hospital...
In this work, we propose a multi-task recurrent neural network with attention mechanism for predicting cardiovascular events from electronic health records (EHRs) at different time horizons. The proposed approach is compared to standard clinical risk predictor (QRISK) and machine learning alternatives using 5-year data NHS Foundation Trust. model outperforms scores in stroke (AUC=0.85) myocardial infarction (AUC=0.89), considering the largest horizon. Benefit of an \gls{mt} setting becomes...
Deprivation of essential nutrients can have stark consequences for many processes in a cell. We consider amino acid starvation, which result bottlenecks mRNA translation when ribosomes stall due to lack resources, i.e. tRNAs charged with the missing acid. Recent experiments also show less obvious effects such as increased charging other (non-starved) tRNA species and selective isoaccepting tRNAs. present mechanism accounts these observations, shows that production some proteins actually...
Abstract The size of a cell sets the scale for all biochemical processes within it, thereby affecting cellular fitness and survival. Hence, needs to be kept certain limits relatively constant over multiple generations. However, how cells measure their use this information regulate growth division remains controversial. Here, we present two mechanistic mathematical models budding yeast ( S. cerevisiae ) cycle investigate competing hypotheses on control: inhibitor dilution titration nuclear...
Abstract Proliferating cells need to coordinate cell division and growth maintain size homeostasis. Any systematic deviation from a balance between results in progressive changes of over subsequent generations. While most eukaryotic execute binary after mass doubling, the photosynthetic green alga Chlamydomonas can grow more than eight-fold during daytime before undergoing rapid cycles DNA replication, mitosis at night, which produce up 16 daughter cells. Here, we propose mechanistic model...