A5015285436- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Neuroscience and Neural Engineering
- Cardiac electrophysiology and arrhythmias
- Congenital heart defects research
- Ion channel regulation and function
- Biomedical Ethics and Regulation
- Enzyme Structure and Function
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Reproductive Biology and Fertility
- Pancreatic function and diabetes
- CAR-T cell therapy research
- Protein Kinase Regulation and GTPase Signaling
- Animal Genetics and Reproduction
- Plant Genetic and Mutation Studies
- Receptor Mechanisms and Signaling
- Xenotransplantation and immune response
- Metalloenzymes and iron-sulfur proteins
- Autophagy in Disease and Therapy
Sheba Medical Center
2025
European Union
2024
Cell and Gene Therapy Catapult
2023-2024
Catalent (United States)
2023-2024
Max Planck Institute for Molecular Biomedicine
2014-2023
Chemical Genomics Centre
2012-2023
Max Planck Society
2012-2023
Max Planck Institute of Biophysics
2020
Erasmus MC Cancer Institute
2019
Danylo Halytsky Lviv National Medical University
2017
Abstract The POU domain transcription factor OCT4 is a key regulator of pluripotency in the early mammalian embryo and highly expressed inner cell mass blastocyst. Consistent with its essential role maintaining pluripotency, Oct4 expression rapidly downregulated during formation trophoblast lineage. To enhance our understanding molecular basis this differentiation event humans, we used functional genomics approach involving RNA interference-mediated suppression function human ESC line...
Abstract Cardiac tissue engineering is a promising strategy to treat heart failure. Yet, several issues remain be resolved including the prevention of arrhythmia caused by inefficient electrical coupling within graft and between host tissue. Here, biohybrid hydrogel composed collagen, alginate, electroconductive poly(3,4‐ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) developed that exhibits extracellular matrix–mimetic fibrous structures enhanced as well cardiomyocyte maturation....
Abstract Fibroblast growth factor 2 (FGF2) is known to promote self-renewal of human embryonic stem cells (hESCs). In addition, it has been shown that transforming β (TGFβ) signaling crucial in the TGFβ/Activin/Nodal branch pathway needs be activated and bone morphogenic protein (BMP)/GDF repressed prevent differentiation. This holds particularly true for Serum Replacement-based medium containing BMP-like activity. We have reinvestigated a widely used protocol conditioning hESC with mouse...
Significance There are few laboratory models that recapitulate human cardiac disease. Here, we created cell for Jervell and Lange-Nielsen syndrome (JLNS) in vitro, based on induced pluripotent stem cells (hiPSCs). JLNS is one of the most severe disorders heart rhythm can cause sudden death young patients. inherited recessively caused by homozygous mutations slow component delayed rectifier potassium current, I Ks . Cardiomyocytes (CMs) from two independent sets patient-derived engineered...
Adult human neural crest-derived stem cells (NCSCs) are of extraordinary high plasticity and promising candidates for the use in regenerative medicine. Here we describe first time a novel cell population within respiratory epithelium adult inferior turbinate. In contrast to superior middle turbinates, amounts source material could be isolated from turbinates. Using minimally-invasive surgery methods isolation is efficient even older patients. Within their endogenous niche, turbinate (ITSCs)...
Long QT syndrome is a potentially life-threatening disease characterized by delayed repolarization of cardiomyocytes, interval prolongation in the electrocardiogram, and high risk for sudden cardiac death caused ventricular arrhythmia. The genetic type 3 this (LQT3) gain-of-function mutations SCN5A sodium channel gene which mediates fast Nav1.5 current during action potential initiation. Here, we report analysis LQT3 human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)....
Abstract Much of the ADP-Glc required for starch synthesis in plastids cereal endosperm is synthesized cytosol and transported across plastid envelope. To provide information on nature role plastidial transporter barley (Hordeum vulgare), we screened a collection low-starch mutants lines with abnormally high levels developing endosperm. Three independent were discovered, all which carried mutations at lys5 locus. Plastids isolated from able to synthesize normal rates Glc-1-P but not ADP-Glc,...
Abstract Epiblast stem cells (EpiSCs) are pluripotent derived from mouse postimplantation embryos at embryonic day (E) 5.5–E7.5 the onset of gastrulation, which makes them a valuable tool for studying mammalian development in vitro. EpiSCs can also be reprogrammed into cell (mESC)-like state. Some reports have shown that reversion requires transcription factor overexpression, whereas others suggested use stringent mESC culture conditions alone is sufficient EpiSCs. To clarify these...
Background Normal development of the atria requires left-right differentiation during embryonic development. Reduced expression Pitx2c (paired-like homeodomain transcription factor 2, isoform c), a key regulator asymmetry, has recently been linked to atrial fibrillation. We therefore systematically studied molecular composition left and right tissue in adult murine human atria. Methods compared gene healthy, mice different strains ages by employing whole genome array analyses on freshly...
Directed cardiac differentiation of human pluripotent stem cells (hPSCs) enables disease modeling, investigation cardiogenesis, as well large-scale production cardiomyocytes (CMs) for translational purposes. Multiple CM protocols have been developed to individually address specific requirements these diverse applications, such enhanced purity at a small scale or mass larger scale. However, there is no universal high-efficiency procedure generating CMs both in two-dimensional (2D) and...
Human pluripotent stem cells (hPSCs) are conventionally grown in a mouse feeder cell-dependent manner. Chemically defined culture conditions are, however, desirable not only for potential medically oriented applications but also investigating mechanisms of self-renewal and differentiation. In light the rather high complexity cost existing hPSC systems, we have systematically evaluated over 20 media ingredients. Only components that reproducibly gave beneficial effects were ultimately...