A5059703104- Sepsis Diagnosis and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- Neonatal and Maternal Infections
- Proteoglycans and glycosaminoglycans research
- Adrenal Hormones and Disorders
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Cystic Fibrosis Research Advances
- Sex and Gender in Healthcare
- Pressure Ulcer Prevention and Management
- Wound Healing and Treatments
- Dialysis and Renal Disease Management
- Plant Pathogens and Resistance
- Diabetic Foot Ulcer Assessment and Management
- Protease and Inhibitor Mechanisms
- Adenosine and Purinergic Signaling
- Antibiotics Pharmacokinetics and Efficacy
- Blood disorders and treatments
- Drug Transport and Resistance Mechanisms
- Climate Change and Health Impacts
- Veterinary Pharmacology and Anesthesia
- Health Policy Implementation Science
Thrombosis and Atherosclerosis Research Institute
2018-2025
McMaster University
2018-2025
Government Medical College
2024
Despite increasing ethical standards for conducting animal research, death is still often used as an endpoint in mouse sepsis studies. Recently, the Murine Sepsis Score (MSS), Mouse Clinical Assessment (M-CASS), and Grimace Scale (MGS) were developed surrogate scoring systems assessing pain disease severity mice. The objective of our study was to compare effectiveness these monitoring body temperature predicting progression cecal ligation puncture (CLP) model, order better inform selection...
Excessive production of neutrophil extracellular traps (NETs) in sepsis contributes to vascular occlusion by acting as a scaffold and stimulus for thrombus formation. Removal DNA, the major structural component NETs, DNase I may reduce host injury.(1) To determine how heparin variants (unfractionated heparin, enoxaparin, Vasoflux, fondaparinux) affect activity, (2) measure temporal changes circulating DNA septic patients.DNA–histone complexes were treated with ± visualized via agarose gels....
Preclinical sepsis models have been criticized for their inability to recapitulate human and suffer from methodological shortcomings that limit external validity reproducibility. The National Sepsis Platform (NPSP) is a consortium of basic science researchers, veterinarians, stakeholders in Canada undertaking standardized multi-laboratory research increase the efficacy efficiency bench-to-bedside translation. In this study, we aimed develop characterize 72-h fecal-induced peritonitis (FIP)...
ABSTRACT Introduction: Cell-free DNA (CFDNA) has emerged as a prognostic biomarker in patients with sepsis. Circulating CFDNA is hypothesized to be associated histones the form of nucleosomes. In vitro, activates coagulation and inhibits fibrinolysis, whereas activate platelets are cytotoxic endothelial cells. Previous studies have targeted or animal models sepsis using DNase I heparins, respectively, which reduced inflammatory thrombosis markers, thereby improving survival. this study, we...
Abstract Despite decades of preclinical research, no experimentally derived therapies for sepsis have been successfully adopted into routine clinical practice. Factors that contribute to this crisis translation include poor representation by models the complex human condition sepsis, bias in studies, as well limitations single-laboratory methodology. To overcome some these shortcomings, multicentre studies—defined a research experiment conducted two or more laboratories with common protocol...
Abstract Background The importance of investigating sex- and gender-dependent differences has been recently emphasized by major funding agencies. Notably, the influence biological sex on clinical outcomes in sepsis is unclear, observational studies suffer from effect confounding factors. controlled experimental environment afforded preclinical allows for clarification mechanistic evaluation sex-dependent differences. We propose a systematic review to assess impact baseline responses disease...
ABSTRACT Background: In sepsis, neutrophil extracellular traps (NETs) are an important interface between innate immunity and coagulation. The major structural component of is nucleosomes (DNA-histone complexes). vitro, DNA histones exert procoagulant/cytotoxic effects whereas not harmful. However, whether DNA, histones, and/or harmful in vivo remain unclear. Objectives: (1) aims the study to investigate cytotoxic ± DNase I heparin vitro (2) when injected into healthy septic mice. Methods :...
Abstract Background: Preclinical sepsis models have been criticized for their inability to recapitulate human and suffer from methodological shortcomings that limit external validity reproducibility. The National Sepsis Platform (NPSP) is a consortium of basic science researchers, veterinarians, stakeholders in Canada undertaking standardized multi-laboratory research increase the efficacy efficiency bench-to-bedside translation. In this study, we aimed develop characterize 72-hour...
Despite older adults being more vulnerable to sepsis, most preclinical research on sepsis has been conducted using young animals. This results in decreased scientific validity since age is an independent predictor of poor outcome. In this study, we explored the impact aging host response fecal-induced peritonitis (FIP) model developed by National Preclinical Sepsis Platform (NPSP). C57BL/6 mice (3 or 12 months old) were injected intraperitoneally with rat fecal slurry (0.75 mg/g) a control...
A 50-year-old male patient, resident of Gondia, Maharashtra, India, presented with chief complaints swelling and raw areas over left foot for 3 months. Patient was apparently alright months prior when he had a traumatic injury piece wood which went across his whole foot, removed by patient himself, then visited private hospital where given various medications, gave him partial relief. After few days, the developed painful up to ankle, associated difficulty in walking local rise temperature,...