A5068677676- Amino Acid Enzymes and Metabolism
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Drug Transport and Resistance Mechanisms
- Polyamine Metabolism and Applications
- Metabolism and Genetic Disorders
- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Sulfur Compounds in Biology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Research and Treatments
- Chemical Synthesis and Analysis
- Molecular Sensors and Ion Detection
- Biomedical Research and Pathophysiology
- Immune cells in cancer
- Peroxisome Proliferator-Activated Receptors
- Bone Metabolism and Diseases
- Electron Spin Resonance Studies
- Sirtuins and Resveratrol in Medicine
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Renin-Angiotensin System Studies
- Pregnancy and preeclampsia studies
University of Lübeck
2022
Heinrich Heine University Düsseldorf
2022
Philipps University of Marburg
2022
University of Münster
2022
Johannes Gutenberg University Mainz
2011-2021
University Medical Center of the Johannes Gutenberg University Mainz
2011-2021
German Centre for Cardiovascular Research
2015
Institute of Molecular Biology and Biophysics
2013
Institute of Pharmacology
2008-2010
Brigham and Women's Hospital
1991-2001
Objective— The present study was conducted to investigate the contribution of perivascular adipose tissue (PVAT) vascular dysfunction in a mouse model diet-induced obesity. Approach and Results— Obesity induced male C57BL/6J mice with high-fat diet for 20 weeks, function studied myograph. In PVAT-free aortas isolated from obese mice, endothelium-dependent, nitric oxide–mediated vasodilator response acetylcholine remained normal. contrast, clear reduction observed when PVAT left place....
Retrovirus infection is initiated by binding of the viral envelope glycoprotein to a cell-surface receptor. The proteins type C retroviruses mammals demonstrate similarities in structural organization and protein sequence. These suggest possibility that from different interference groups might use related as receptors, despite absence any relationship between retrovirus receptors isolated date. To investigate this possibility, we have identified human cDNA clone encoding closely receptor for...
The current study was designed to investigate the importance of cationic amino acid transporters (CATs) for L-arginine supply nitric oxide (NO) synthases in mouse J774A.1 macrophages and human EA.hy926 endothelial cells. CAT-1 expressed both cell types, whereas CAT-2B only activated macrophages. Apparent K(M) values transport types consistent with expression system y(+) carriers (and macrophages). In addition, Na(+) independent sensitive trans-stimulation. A 2-h preincubation 2 mM L-lysine...
The liver regulates the supply of amino acids required for protein synthesis and intermediary metabolism between feeding fasting in mammals. flux other tissues is determined, part, by activity specific carrier proteins. We have identified a cationic arginine, lysine, ornithine mouse that closely related to previously transporter with same substrate specificity expressed nonhepatic tissues. Uptake studies were performed Xenopus oocytes injected cRNA encoding these comparison two transporters...
The cartilagenous tissue of mandibular condyles newborn mice contains progenitor cells as well young and mature chondrogenic cells. During in vitro cultivation the tissue, undergo osteogenic differentiation form new bone (Silbermann, M., D. Lewinson, H. Gonen, M. A. Lizarbe, K. von der Mark. 1983. Anat. Rec. 206:373-383). We have studied expression genes that typify during cultivation. RNAs for collagen type I, osteonectin, alkaline phosphatase, gla protein were sequentially expressed...
A crucial cause of the decreased bioactivity nitric oxide (NO) in cardiovascular diseases is uncoupling endothelial NO synthase (eNOS) caused by oxidative stress-mediated deficiency NOS cofactor tetrahydrobiopterin (BH(4)). The reversal eNOS might represent a novel therapeutic approach. treatment apolipoprotein E knockout (ApoE-KO) mice with resveratrol resulted up-regulation superoxide dismutase (SOD) isoforms (SOD1-SOD3), glutathione peroxidase 1 (GPx1), and catalase down-regulation NADPH...
Cytokine-dependent production of nitric oxide (NO) by rat cardiac myocytes is a consequence increased expression the inducible isoform synthase (iNOS or NOS2) and, in presence insulin, depresses contractile function these cells vivo and vitro. Experiments reported here show that L-lysine, competitive antagonist L-arginine uptake, suppressed NO (detected as nitrite accumulation) interleukin (IL)-1β interferon (IFN)γ-pretreated 70%, demonstrating dependent on uptake. Cardiac constitutively...
W e have identified the third member of a family cationic amino acid transporters in lipopolysaccharidestimulated murine macrophages.The deduced sequence this transporter is same as MCAT-2 (mouse transporter-Z), low affinity expressed hepatocytes, except for stretch 41 acids that connects eighth and ninth membrane-spanning domains.These apparently result from differential splicing transcripts single gene therefore been named MCAT-2A (hepatocyte) MCAT-2B (macrophage).Despite their similarity,...
In this study, we aimed at analyzing the human homologues of murine cationic amino acid transporters mCAT-1, mCAT-2A, and mCAT-2B. cDNAs encoding hCAT-1 had been previously reported by two independent groups [Albritton, L.M., et al. (1993) Genomics 12, 430; Yoshimoto, T., (1991) Virology 185, 10]. We isolated hCAT-2A hCAT-2B from a liver cDNA library derived hepatoma cell line HepG2, respectively. Analyses deduced sequences both carriers demonstrated 90.9% identity with respective proteins....
The expression of NOS isoforms was studied in guinea pig skeletal muscle at the mRNA and protein level, effect NO on contractile response examined. Ribonuclease protection analyses demonstrated I II mRNAs diaphragm gastrocnemius muscle. In Western blots, immunoreactivities were found particulate but not soluble fraction activity almost exclusively fraction. About 50% this Ca2+ independent. immunohisto- chemistry, anti-NOS antibody stained distinct membrane regions fibers. most intense...