A5076408876- Chemical and Physical Properties in Aqueous Solutions
- Spectroscopy and Quantum Chemical Studies
- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Research in Cotton Cultivation
- Molecular spectroscopy and chirality
- Dietary Effects on Health
- Photosynthetic Processes and Mechanisms
- Lipid Membrane Structure and Behavior
- Microbial Metabolic Engineering and Bioproduction
- Exercise and Physiological Responses
- Protein Structure and Dynamics
- RNA Research and Splicing
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Heat shock proteins research
- Cardiovascular and exercise physiology
- Protein Interaction Studies and Fluorescence Analysis
- Chemistry and Chemical Engineering
- nanoparticles nucleation surface interactions
- Neurobiology and Insect Physiology Research
- Various Chemistry Research Topics
- Regulation of Appetite and Obesity
- Diet and metabolism studies
- Advanced Mathematical Modeling in Engineering
Cleveland Research (United States)
2021-2024
Kent State University
2018-2023
The organization of multiple subcellular compartments is controlled by liquid–liquid phase separation.
Liquid-liquid phase separation underlies the formation of membrane-less organelles inside living cells. The mechanism this process can be examined using simple aqueous mixtures two or more solutes, which are able to separate at specific concentration thresholds. This work presents first experimental evidence that mesoscopic changes precede visually detected macroscopic in polymers and a single polymer salt. Dynamic light scattering (DLS) analysis indicates agglomerates these systems. These...
Trimethylamine- N -oxide enhances or suppresses phase separation in aqueous two-phase systems depending on the composition and concentration of forming components.
The partition behavior of single and double-point mutants bacteriophage T4 lysozyme (T4 lysozyme) staphylococcal nuclease A was examined in different aqueous two-phase systems (ATPSs) studied by Solvent Interaction Analysis (SIA). Additionally, the solvent accessible surface area (SASA) modeled both proteins calculated. silico calculations vitro analyses correlate, indicating that analysis ATPSs provides a valid descriptor (SIA signature) covering various protein features, such as structure,...
Non-shivering thermogenesis can promote negative energy balance and weight loss. In this study, we identified a contextual stimulus that induces rapid robust in skeletal muscle. Rats exposed to the odor of natural predator (ferret) showed elevated muscle temperatures detectable as quickly 2 min after exposure, reaching maximum >1.5°C at 10-15 min. Mice exhibited similar thermogenic response same odor. Ferret induced significantly larger qualitatively different from novel or aversive odors,...
Lipid droplets (LDs) are ubiquitously expressed organelles; the only intracellular organelles that contain a lipid monolayer rather than bilayer. Proteins localize and bind to this as they do bilayers. The mechanism by which cytosolic LD binding proteins recognize, bind, interface remains poorly understood. Amphipathic α-helix bundles form common motif is shared between (e.g., perilipins 2, 3, 5) apolipoproteins, such apoE apoLp-III, found on lipoprotein particles. Here, we use pendant drop...
Analysis by attenuated total reflection–Fourier transform infrared spectroscopy shows that each coexisting phase in aqueous two-phase systems has a different arrangement of hydrogen bonds. Specific arrangements vary for formed solutes. The bond is shown to correlate with differences hydrophobic and electrostatic properties the phases five specific systems, four two polymers one single polymer salt. results presented here suggest bonds may be an important factor separation.
This work presents the first evidence that dissolved globular proteins change arrangement of hydrogen bonds in water, with different showing quantitatively effects. Using ATR-FTIR (Attenuated Total Reflection – Fourier Transform Infrared) spectroscopic analysis OH-stretch bands, we obtain quantitative estimates relative amounts previously reported four subpopulations water structures coexisting a variety aqueous solutions. Where solvatochromic dyes can measure properties solutions...
Aqueous two-phase systems (ATPSs) are formed when two nonionic polymers, or a single polymer and salt, mixed in water above specific concentration, resulting the emergence of phase separation formation immiscible aqueous phases. The solvent properties media within phases ATPSs rely on composition co-solutes arrangement hydrogen bond network each phase. Here, we investigate anion effect various sodium salts enhancement destabilization polyethylene glycol (PEG)–salt ATPS formation. Relatively...
This work presents the first evidence that dissolved globular proteins change arrangement of hydrogen bonds in water, with different showing quantitatively effects. Using ATR-FTIR (attenuated total reflection-Fourier transform infrared) spectroscopic analysis OH-stretch bands, we obtain quantitative estimates relative amounts previously reported four subpopulations water structures coexisting a variety aqueous solutions. Where solvatochromic dyes can measure properties solutions non-ionic...
Weight and fat loss occur when total energy expenditure (EE) is higher than input. This can be achieved by lowering caloric input through altering diet, or increasing EE. Activity‐associated EE suppressed during weight maintenance of reduced weight; this occurs decreased physical activity levels along with increased fuel economy activity. These adaptations take time to occur. Both activity‐associated resting have been shown decrease calorie restriction‐induced in rats. Recently, attention...