A5015537376- Selenium in Biological Systems
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Folate and B Vitamins Research
- Blood Coagulation and Thrombosis Mechanisms
- Trace Elements in Health
- Neurological Disease Mechanisms and Treatments
- Protease and Inhibitor Mechanisms
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Neuroscience and Neuropharmacology Research
- Advanced biosensing and bioanalysis techniques
- Medical Research and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- DNA and Nucleic Acid Chemistry
- Birth, Development, and Health
- ATP Synthase and ATPases Research
- Ferroptosis and cancer prognosis
- Neonatal and fetal brain pathology
- Nitrogen and Sulfur Effects on Brassica
- Disaster Response and Management
- Retinoids in leukemia and cellular processes
University of Illinois Chicago
2016-2024
Midwestern University
2013-2020
Northwestern University
1999
Abstract Endothelin-B receptor agonist, IRL-1620, provides significant neuroprotection following cerebral ischemia in rats. Whether this is due to inhibition of apoptosis unknown. IRL-1620-treated rats permanent middle artery occlusion (MCAO) showed improvement neurological and motor functions along with a decrease infarct volume at 24 h (−81.3%) day 7 (−73.0%) compared vehicle group. Cerebral blood flow (CBF) significantly improved animals by post MCAO. an increase phospho-Akt Bad level...
Seryl-tRNA synthetase (SerRS) attaches L-serine to the cognate serine tRNA (tRNA Ser ) and noncognate selenocysteine Sec ). The latter activity initiates anabolic cycle of (Sec), proper decoding an in-frame UGA codon, synthesis selenoproteins across all domains life. While accuracy SerRS is important for overall proteome integrity, it its substrate promiscuity that vital integrity selenoproteome. This raises a question as what elements in two species, harboring different anticodon sequences...
Abstract Selenocysteine synthase (SepSecS) catalyzes the terminal reaction of selenocysteine, and is vital for human selenoproteome integrity. Autosomal recessive inheritance mutations in SepSecS–Ala239Thr, Thr325Ser, Tyr334Cys Tyr429*–induced severe, early-onset, neurological disorders distinct populations. Although harboring different mutant alleles, patients presented remarkably similar phenotypes typified by cerebellar cerebral atrophy, seizures, irritability, ataxia, extreme spasticity....
Abstract O-Phosphoseryl-tRNASec selenium transferase (SepSecS) catalyzes the terminal step of selenocysteine (Sec) synthesis in archaea and eukaryotes. How Sec synthetic machinery recognizes discriminates tRNASec from tRNA pool is essential to integrity selenoproteome. Previously, we suggested that SepSecS adopts a competent conformation pre-ordered for catalysis. Herein, using high-resolution X-ray crystallography, visualized tRNA-dependent conformational changes human may be prerequisite...
Abstract Although genomes encode instructions for mammalian cell differentiation with rich syntactic relationships, existing methods genetically programming cells have modest capabilities stepwise regulation of genes. Here, we developed a sequential genetic system that enables transcriptional activation endogenous genes in preprogrammed, manner. The relies on the removal an RNA polymerase III termination signal to induce both and DNA endonuclease activities Cas9-VPR protein effect...
The evolution of the genetic code to incorporate selenocysteine (Sec) enabled development a selenoproteome in all domains life. O-phosphoseryl-tRNASec selenium transferase (SepSecS) catalyzes terminal reaction Sec synthesis on tRNASec archaea and eukaryotes. Despite harboring four equivalent active sites, human SEPSECS binds no more than two molecules. Though, basis for this asymmetry remains poorly understood. In humans, an acidic, C-terminal, α-helical extension precludes additional...
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Selenium is an essential micronutrient for human health. The biologically active form of selenium in all domains life the 21 st amino acid, selenocysteine (Sec). incorporation Sec into proteins unique compared to standard acids as encoded by a UGA stop codon and directly synthesized from serine precursor on its cognate tRNA, tRNA . Although not well understood, biosynthesis requires three enzymes archaea eukaryotes. enzyme synthase (SepSecS) tetrameric that catalyzes terminal reaction...