A5058935789- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Genomics and Phylogenetic Studies
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Diagnosis and Treatment of Venous Diseases
- Soft tissue tumor case studies
- Tumors and Oncological Cases
- Salivary Gland Tumors Diagnosis and Treatment
- Toxin Mechanisms and Immunotoxins
- Neuroendocrine Tumor Research Advances
- Cardiac, Anesthesia and Surgical Outcomes
- Biopolymer Synthesis and Applications
- Probiotics and Fermented Foods
- Neurofibromatosis and Schwannoma Cases
- Venous Thromboembolism Diagnosis and Management
- Cellular transport and secretion
- RNA regulation and disease
Institute of Bioorganic Chemistry
2008-2024
Russian Academy of Sciences
2005-2011
Ribosomal proteins (r-proteins) are abundant, highly conserved, and multifaceted cellular in all domains of life. Most r-proteins have RNA-binding properties can form protein–protein contacts. Bacterial govern the co-transcriptional rRNA folding during ribosome assembly participate formation functional sites, such as mRNA-binding site, tRNA-binding peptidyl transferase center, protein exit tunnel. In addition to their primary role a cell integral components synthesis machinery, many function...
Autogenous regulation is a general strategy of balancing ribosomal protein synthesis in bacteria. Control mechanisms have been studied detail for most operons, except rpsB-tsf encoding essential r-protein S2 and elongation factor Ts, where even the promoter has remained unknown. By using single-copy translational fusions with chromosomal lacZ gene Western-blot analysis, we demonstrate here that serves as negative regulator both rpsB tsf expression vivo, acting at single target within...
It is widely assumed that in the best-characterized model bacterium Escherichia coli, transcription units encoding ribosomal proteins (r-proteins) and regulation of their expression have been already well defined. However, start sites for several E. coli r-protein operons established only very recently, so information concerning these at transcriptional or posttranscriptional level still missing. This paper describes first time vivo three operons, rplM-rpsI, rpmB-rpmG, rplU-rpmA The results...
Ribosomal proteins (r-proteins) are abundant, highly conserved, and multifaceted cellular in all domains of life. Most r-proteins have RNA-binding properties able to form protein-protein contacts. Bacterial govern the co-transcriptional rRNA folding during ribosome assembly participate formation functional sites such as mRNA binding site, tRNA sites, peptidyl- transferase center, protein exit tunnel. In addition their primary role a cell integral components synthesis machinery, many can...
Ribosomal protein (r-protein) L25 is one of the three r-proteins (L25, L5, L18) that interact with 5S rRNA in eubacteria. Specific binding a certain domain r-RNA, so-called loop E, has been studied detail, but information about regulation synthesis remained totally lacking. In contrast to rplE (L5) and rplR (L18) genes belong polycistronic spc -operon are regulated at translation level by r-protein S8, rplY (L25) gene forms an independent transcription unit. The main goal this work was study...
Small non-coding RNAs play a key role in bacterial adaptation to various stresses. Mycobacterium tuberculosis small RNA MTS1338 is upregulated during mycobacteria infection of macrophages, suggesting its involvement the interaction pathogen with host. In this study, we explored functional effects by expressing it non-pathogenic smegmatis that lacks gene. The results indicated slowed growth recombinant culture and increased their survival RAW 264.7 where MTS1338-expressing strain...
Abstract Progress in RNA metabolism and function studies relies largely on molecular imaging systems, including those comprising a fluorogenic dye an aptamer-based fluorescence-activating tag. G4 aptamers of the Mango family, typically combined with duplex/hairpin scaffold, activate fluorescence green light-emitting TO1-biotin hold great promise for intracellular tracking. Here, we report new Mango-based platform. Its key advantages are tunability spectral properties applicability...
Bacterial ribosomal proteins (r-proteins) encoded by nonessential genes often carry out very important tasks in translation. In particular, this is the case of a small basic bacteria-specific r-protein L31 (bL31). Recent studies revealed crucial role bL31 formation protein–protein intersubunit bridge B1b and hence its contribution to ribosome dynamics. Our goal was study vivo regulation rpmE operon encoding bL31. We used previously developed approach based on chromosomally integrated fusions...
Ribosomal protein S2 is an essential component of translation machinery, and its viable mutated variants conferring distinct phenotypes serve as a valuable tool in studying the role regulation. One few available rpsB mutants, rpsB1, shows thermosensitivity ensures enhanced expression leaderless mRNAs. In this study, we identified nature rpsB1 mutation. Sequencing allele revealed G-to-A transition part gene which encodes coiled-coil domain S2. The resulting E132K substitution resides highly...
Bacterial adaptation to cold stress requires wide transcriptional reprogramming. However, the knowledge of molecular mechanisms underlying response mycobacteria is limited. We conducted comparative transcriptomic analysis Mycobacterium smegmatis subjected shock. The growth M. cultivated at 37 °C was arrested just after exposure (acclimation phase) but later (by 24 h) resumed a much slower rate (adaptation phase). Transcriptomic analyses revealed distinct gene expression patterns...
The autogenous regulation of ribosomal protein (r-protein) synthesis plays a key role in maintaining the stoichiometry components bacteria. In this work, taking rpsO gene as classic example, we addressed for first time vivo r-protein mycobacteria M. smegmatis (Msm) and tuberculosis (Mtb). We used strategy based on chromosomally integrated reporters under control regulatory regions ectopic expression Msm S15 to measure its impact reporter expression. Because use E. coli host appeared...
ABSTRACT Autogenous regulation of ribosomal protein (r-protein) synthesis plays a key role in maintaining the stoichiometry components bacteria. Our main goal was to develop techniques for investigating r-protein mycobacteria, Gram-positive organisms with high GC-content, which has never been addressed. We started rpsO gene known be autoregulated by its product, S15, broad range bacterial species. To study vivo from Mycobacterium smegmatis ( Msm ), we first applied an approach based on...
Abstract Progress in the studies of RNA metabolism and function relies largely on molecular imaging systems, including those comprising a fluorogenic dye an aptamer-based fluorescence-activating tag. G4 aptamers Mango family, typically combined with duplex/hairpin scaffold, activate fluorescence green-emitting TO1-biotin hold great promise for intracellular tracking. Here, we report new Mango-based platform. Its key advantages are tunability spectral properties applicability visualization...