A5080294129- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Research and Splicing
- Osteoarthritis Treatment and Mechanisms
- RNA and protein synthesis mechanisms
- Extracellular vesicles in disease
- Viral Infections and Immunology Research
- MicroRNA in disease regulation
- Air Quality and Health Impacts
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- interferon and immune responses
- Energy and Environment Impacts
- Genetic Syndromes and Imprinting
- Sarcoidosis and Beryllium Toxicity Research
- Cytokine Signaling Pathways and Interactions
- Knee injuries and reconstruction techniques
- Circular RNAs in diseases
- Bone and Joint Diseases
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Global Health and Surgery
- Biotechnology and Related Fields
- Tissue Engineering and Regenerative Medicine
- Total Knee Arthroplasty Outcomes
- Bone health and treatments
University of Liverpool
2023-2025
Maastricht University
2019-2024
Centre for Life
2024
Orthopedic One
2023
Maastricht University Medical Centre
2020-2021
Palacký University Olomouc
2016-2020
Perlecan (HSPG2), a heparan sulfate proteoglycan similar to agrin, is key for extracellular matrix (ECM) maturation and stabilization. Although crucial cardiac development, its role remains elusive. We show that perlecan expression increases as cardiomyocytes mature in vivo during human pluripotent stem cell differentiation (hPSC-CMs). Perlecan-haploinsuffient hPSCs (HSPG2+/−) differentiate efficiently, but late-stage CMs have structural, contractile, metabolic, ECM gene dysregulation. In...
Abstract Osteoarthritis presents as a change in the chondrocyte phenotype and an imbalance between anabolic catabolic processes. Age affects its onset progression. Small nucleolar RNAs (SnoRNAs) direct chemical modification of RNA substrates to fine-tune spliceosomal rRNA function, accommodating changing requirements for splicing protein synthesis during health disease. Articular cartilage from young, old OA knees was used microarray study identify alterations snoRNA expression. Changes...
Small nucleolar RNAs (snoRNAs) are emerging as important regulators of cardiovascular (patho)biology. Several roles snoRNAs have recently been identified in heart development and congenital diseases, well their dynamic regulation hypertrophic dilated cardiomyopathies, coronary disease (CHD), myocardial infarction (MI), cardiac fibrosis, failure. Furthermore, reports changes vesicular snoRNA expression altered levels circulating response to stress suggest that also function signaling...
ObjectiveOsteoarthritis-related cartilage extracellular matrix remodeling is dependent on changes in chondrocyte protein expression. Yet, the role of ribosomes translation regulation unknown. In this exploratory study, we investigated ribosomal RNA (rRNA) epitranscriptomic-based ribosome heterogeneity human articular chondrocytes and its relevance for osteoarthritis.MethodsSequencing-based rRNA 2′-O-methylation profiling analysis (RiboMethSeq) was performed non-OA primary (n = 5) exposed 14...
Abstract Although pathways controlling ribosome activity have been described to regulate chondrocyte homeostasis in osteoarthritis, biogenesis osteoarthritis is unexplored. We hypothesized that U3 snoRNA, a non-coding RNA involved ribosomal maturation, critical for protein translation capacity osteoarthritis. snoRNA was one of number snoRNAs with decreased expression osteoarthritic cartilage and chondrocytes. OA synovial fluid impacted by affecting gene promoter activity, while BMP7 able...
Purpose. Pulmonary sarcoidosis is associated with dysregulated expression of intracellular miRNAs. There however only little information on extracellular miRNAs and their association the disease course in sarcoidosis. We therefore assessed serum classified according to presence Löfgren's syndrome (LS) as a hallmark good prognosis contrast more advanced course. Methods. RT-PCR was used assess 35 13 healthy controls 24 patients (12 X-ray (CXR) stage ≤ 1 LS 12 insidious onset CXR ≥ 3). Results....
Extracellular vesicles (EVs) contribute to osteoarthritis pathogenesis through their release into joint tissues and synovial fluid. Synovial fluid-derived EVs have the potential be direct biomarkers in causal pathway of disease but also enable understanding role progression. Utilizing a temporal model osteoarthritis, we defined changes matched fluid plasma-derived EV small non-coding RNA protein cargo using sequencing mass spectrometry. Data exploration included time series clustering,...
Objective Osteoarthritis (OA) is characterized by articular cartilage erosion, pathological subchondral bone changes, and signs of synovial inflammation pain. We previously identified p[63-82], a morphogenetic protein 7 (BMP7)-derived bioactive peptide that attenuates structural degeneration in the rat medial meniscal tear-model for posttraumatic OA. This study aimed to evaluate erosion-attenuating activity p[63-82] different preclinical model OA (anterior cruciate ligament...
Alterations in cell fate are often attributed to (epigenetic) regulation of gene expression. An emerging paradigm focuses on specialized ribosomes within a cell. However, little evidence exists for the dynamic ribosome composition and function. Here, we stimulated chondrocytic line with transforming growth factor beta (TGF-β2) mapped changes function, ribosomal RNA (rRNA) epitranscriptomics. 35S Met/Cys incorporation was used evaluate activity. Dual luciferase reporter assays were assess...
Abstract Extracellular vesicles contribute to osteoarthritis pathogenesis through their release into joint tissues and synovial fluid. Limited studies have profiled extracellular in osteoarthritic biofluids, such as plasma Due the potential involvement pathogenesis, fluid-derived be ‘direct’ biomarkers causal pathway of disease but also enable understanding role progression. Utilizing a temporal model early osteoarthritis, we defined changes matched fluid plasma-derived vesicle small...
Eukaryotic ribosomes are complex molecular nanomachines translating genetic information from mRNAs into proteins. There is natural heterogeneity in ribosome composition. The pseudouridylation (ψ) of ribosomal RNAs (rRNAs) one the key sources heterogeneity. Nevertheless, functional consequences ψ-based and its relevance for human disease yet to be understood. Using HydraPsiSeq a chronic model non-osteoarthritic primary articular chondrocytes exposed osteoarthritic synovial fluid, we...
ABSTRACT Objectives Osteoarthritis (OA) presents as a change in the chondrocyte phenotype and an imbalance between anabolic catabolic processes. Age affects its onset progression. Small nucleolar RNAs (SnoRNAs) direct chemical modification of RNA substrates to fine-tune spliceosomal rRNA function, accommodating changing requirements for splicing protein synthesis during health disease. This study was undertaken examine how changes snoRNAs expression may have role OA. Methods Articular...
Mutations in the non-coding snoRNA component of mitochondrial RNA processing endoribonuclease (RMRP) are cause cartilage-hair hypoplasia (CHH). CHH is a rare form metaphyseal chondrodysplasia characterized by disproportionate short stature and abnormal growth plate development. The process chondrogenic differentiation within plates long bones vital for longitudinal bone growth. However, molecular mechanisms behind impaired skeletal development patients remain unclear. We employed...
1. Abstract Extracellular vesicles (EVs) allow the exchange of protein, lipids and genetic material for communication between cells. We developed a method to track protein cells via EVs, using stable isotope labelling amino acids in cell culture (SILAC). compared EV isolation methods: ultracentrifugation size exclusion chromatography (SEC) undertook characterisation through nanoparticle tracking analysis (NTA), then optimised requirement (0-10%) foetal calf serum (FCS) application...
The diseases associated with tobacco smoking affect miRNAs and small single-stranded non-coding RNAs. However, there are no data on urinal in healthy smokers. We searched for the possible effect of cessation miRNA urine expression. For screening, Affymetrix 4.0 arrays were used 33 samples obtained from six never smokers current three time-points before (n = 10), after short time abstinence (3-8 weeks), long-term (1 year). validation, a quantitative (q) polymerase chain reaction (PCR) method...
<b>Background:</b> Cigarette smoking and respiratory diseases due to chronic have been associated with several alterations in lung expression of miRNAs; small single-stranded non-coding ribonucleic acids. Although cigarette affects primarily tissue, chemical components smoke are present circulation even urinal samples indicating its possible systemic effect. However, there no data on miRNAs smoking. Hereby we tested urine for presence searched effect cessation. <b>Method:</b> Affymetrix...