A5055686645- Redox biology and oxidative stress
- Rangeland and Wildlife Management
- Metalloenzymes and iron-sulfur proteins
- Microbial Community Ecology and Physiology
- Electrochemical sensors and biosensors
- Sulfur Compounds in Biology
- Biochemical Acid Research Studies
- Metabolism and Genetic Disorders
- Photosynthetic Processes and Mechanisms
- Clay minerals and soil interactions
- Biochemical and Molecular Research
- Photoreceptor and optogenetics research
- Metal-Catalyzed Oxygenation Mechanisms
- Multiple Myeloma Research and Treatments
- Amino Acid Enzymes and Metabolism
- Heme Oxygenase-1 and Carbon Monoxide
- Frailty in Older Adults
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Enzyme Structure and Function
- Ecology and Vegetation Dynamics Studies
- Alcoholism and Thiamine Deficiency
- Wastewater Treatment and Nitrogen Removal
- Fire effects on ecosystems
- Metal Extraction and Bioleaching
- Soil and Unsaturated Flow
University Hospitals of Derby and Burton NHS Foundation Trust
2025
Pomona College
2013-2023
University of Sheffield
2015
Oncology Hematology Care
2009-2010
Sarah Cannon Research Institute
2009
Moffitt Cancer Center
2005
Florida College
2005
University of South Florida
2005
Salisbury University
2001
Wake Forest University
1995-2000
Summary The cellular responses of Borrelia burgdorferi To reactive oxygen species (ROS) encountered during the different stages its infective cycle are poorly understood. Few enzymes responsible for protecting proteins, DNA/RNA and lipids from damage by ROS have been identified characterized. Data presented here suggest that bb0728 encodes an enzyme involved in this process. Biochemical analyses on purified recombinant BB0728 indicated it functioned as a coenzyme A disulphide reductase...
Type zero copper is a hard-ligand analogue of the classical type 1 or blue site in proteins that function as electron transfer (ET) agents photosynthesis and other biological processes. The EPR spectroscopic features CuII are very similar to those copper, although lacking deep color, due absence thiolate ligation. We have measured rates intramolecular ET from pulse radiolytically generated C3−C26 disulfide radical anion both C112D/M121L 2 C112D Pseudomonas aeruginosa azurins pH 7.0 aqueous...
A wealth of H 2 O‐producing NADH oxidase (NOX) homologues have been discovered in the genomes hyperthermophilic Archaea, including two genome Pyrococcus furiosus which designated as NOX1 and NOX2. In order to investigate function NOX1, structural gene encoding was cloned from P. expressed Escherichia coli , resulting recombinant enzyme (rNOX1) purified homogeneity. The is a thermostable flavoprotein that can be reconstituted only with FAD. rNOX1 catalyzes oxidation NADH, producing both O...
Shewanella algae, S. putrefaciens, and Photobacterium damselae subsp. are indigenous marine bacteria human pathogens causing cellulitis, necrotizing fasciitis, abscesses, septicemia, death. Infections rare most often associated with the immunocompromised host. A study was performed on microbiological flora of oysters seawater from commercial oyster harvesting sites in Delaware Bay, New Jersey. From 276 water shellfish samples tested, 1,421 bacterial isolates were picked for biochemical...
We have cloned NADH oxidase homologues from Pyrococcus horikoshii and P. furiosus , purified the recombinant form of enzyme to homogeneity Escherichia coli . Both enzymes (previously referred as NOX2) been shown act a coenzyme A disulfide reductases (CoADR: CoA‐S‐S‐CoA + NAD(P)H H →2CoA‐SH NAD(P) ). The shows k cat app 7.2 s −1 with NADPH at 75 °C. While preference for NADPH, it is able use both efficiently, giving roughly equal s, while K m eightfold lower than that NADH. specific CoA...
In order to characterize the native Cys42-sulfenic acid redox center of flavoprotein NADH peroxidase by NMR, an expression protocol has been developed which yields [3-13C]Cys42-labeled protein in 100 mg quantities. Difference spectra labeled minus unlabeled oxidized enzyme (E) give a peak at 41.3 ppm (relative dioxane) represents acid. Reduction E with 1 equiv gives air-stable two-electron reduced (EH2) species, and difference maxima minima 30.8 ppm, respectively, corresponding -thiolate...
Journal Article Determination of coenzyme A levels in Pyrococcus furiosus and other Archaea: implications for a general role thermophiles Get access Charles S. Hummel, Hummel Department Chemistry, 645 North College Avenue, Pomona College, Claremont, CA 91711, USA Search works by this author on: Oxford Academic PubMed Google Scholar Kyle M. Lancaster, Lancaster Edward J. Crane, III *Corresponding author. Tel.: +1 909 6079634; fax: 6077726. ej.crane@pomona.edu, ejc14747@pomona.edu FEMS...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnalysis of the Kinetic Mechanism Enterococcal NADH Peroxidase Reveals Catalytic Roles for Complexes with both Oxidized and Two-Electron-Reduced Enzyme FormsEdward J. Crane, III, Derek Parsonage, Leslie B. Poole, Al ClaiborneCite this: Biochemistry 1995, 34, 43, 14114–14124Publication Date (Print):October 1, 1995Publication History Published online1 May 2002Published inissue 1 October...
The NADH-dependent persulfide reductase (Npsr), a recently discovered member of the PNDOR family flavoproteins that contains both canonical flavoprotein domain and rhodanese domain, is proposed to be involved in dissimilatory reduction S(0) for Shewanella loihica PV-4. We have previously shown polysulfide substrate this enzyme, determined structure closely related enzyme (CoADR-Rhod from Bacillus anthracis) suggested importance bound coenzyme A mechanism. work described here shows vivo...
FAD and NAD(P)H-dependent coenzyme A disulfide reductases/polysulfide reductases (CoADR/Psr) have been proposed to be important for the reduction of sulfur disulfides in sulfur-reducing anaerobic hyperthermophiles Pyrococcus horikoshii furiosus; however, form(s) that enzyme actually reduces are not clear. Here we determined structure FAD- A-containing holoenzyme from P. 2.7 Å resolution characterized its substrate specificity. The is relatively promiscuous a range disulfide, persulfide,...
In order to test the proposal [Stehle, T., Claiborne, A., & Schulz, G. E. (1993) Eur. J. Biochem. 211, 221−226] that active-site His10 of NADH peroxidase functions as an essential acid−base catalyst, we have analyzed mutants in which this residue has been replaced by Gln or Ala. The kcat values for both H10Q and H10A peroxidases, pH profile with H10Q, are very similar those observed wild-type peroxidase. Both mutants, however, exhibit Km(H2O2) much higher (50−70-fold) than enzyme,...
A novel anaerobic, hyperthermophilic archaeon was isolated from a mud volcano in the Salton Sea geothermal system southern California, USA. The isolate, named strain 521T, grew optimally at 90 °C, pH 5.5-7.3 and with 0-2.0 % (w/v) NaCl, generation time of 10 h under optimal conditions. Cells were rod-shaped non-motile, ranging 2 to 7 µm length. Strain 521T only presence thiosulfate and/or Fe(III) (ferrihydrite) as terminal electron acceptors strictly anaerobic conditions, preferred...
Understanding how wildfires and modification in plant assemblages interact to influence soil bacteria is a crucial step understanding these disturbances may ecosystem structure function. Here, we resampled from three study sites previously surveyed spring 2016 2017 compared bacterial prior six months after (spring 2019) the 2018 Woolsey Fire Santa Monica Mountain National Recreation Area using Illumina sequencing of 16S rRNA gene. All harbored both native California sage scrub non-native...
The crystal structure of the flavoprotein NADH peroxidase shows that Arg303 side chain forms a hydrogen bond with active-site His10 imidazole and is therefore likely to influence catalytic mechanism. Dithionite titration an R303M mutant [E(FAD, Cys42-sulfenic acid)] yields two-electron reduced intermediate (EH2) enhanced flavin fluorescence almost no charge-transfer absorbance at pH 7.0; pKa for nascent Cys42-SH increased by over 3.5 units in comparison wild-type EH2 ≤4.5. same intermediate,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSingle-turnover studies of brewer's yeast pyruvate decarboxylase: C(2)-proton transfer from thiamin diphosphateEdward J. Crane III, Joseph A. Vaccaro, and Michael W. WashabaughCite this: Am. Chem. Soc. 1993, 115, 20, 8912–8917Publication Date (Print):October 1, 1993Publication History Published online1 May 2002Published inissue 1 October 1993https://pubs.acs.org/doi/10.1021/ja00073a004https://doi.org/10.1021/ja00073a004research-articleACS...