A5100651619- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- PARP inhibition in cancer therapy
- HER2/EGFR in Cancer Research
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- Lung Cancer Treatments and Mutations
- Peptidase Inhibition and Analysis
- Galectins and Cancer Biology
- Telomeres, Telomerase, and Senescence
- Neuroblastoma Research and Treatments
- Enzyme Production and Characterization
- Prostate Cancer Treatment and Research
- Microtubule and mitosis dynamics
- Click Chemistry and Applications
- Cancer, Hypoxia, and Metabolism
- PI3K/AKT/mTOR signaling in cancer
- Diet, Metabolism, and Disease
- Ophthalmology and Visual Impairment Studies
- Retinal Diseases and Treatments
- Signaling Pathways in Disease
- Cancer Genomics and Diagnostics
- Chemical Synthesis and Analysis
- Sarcoma Diagnosis and Treatment
- Immune Cell Function and Interaction
Shanghai First Maternity and Infant Hospital
2025
Xiangya Hospital Central South University
2016-2024
Central South University
2016-2024
Wuhan University
2019-2024
Shanghai Institute of Materia Medica
2017-2024
Chinese Academy of Sciences
2017-2024
Renmin Hospital of Wuhan University
2019-2024
Yili Friendship Hospital
2023-2024
University of Chinese Academy of Sciences
2017-2024
Beijing University of Chinese Medicine
2024
Direct chemical modification of native antibodies in a site-specific manner remains great challenge. Ligand-directed conjugation can achieve the selective antibodies, but usually requires multiple extra steps for ligand release and cargo assembly. Herein, we report novel, traceless strategy to enable facile efficient one-step synthesis antibody-drug conjugates (ADCs) by harnessing thioester-based acyl transfer reagent. The designed reagent, consisting an optimized Fc-targeting ligand,...
Although classic Hodgkin lymphoma (cHL) is highly curable with current treatment paradigms, therapy fails in 10-25% of patients. This prospective multicenter phase II study attempted to investigate the efficacy and safety combination tislelizumab gemcitabine oxaliplatin (T-GemOx) relapsed or refractory cHL. Participants received six eight courses (1 g/m2 on day 1) (100 mg/m2 combined (200 mg 2) at 21-day intervals, followed by maintenance (every 2 months for years). The main outcome measure...
Gorham-Stout disease is a life-threatening disorder often manifested by lymphatic malformation and osteolysis. Unfortunately, available therapies are not uniformly effective carry substantial morbidity. We report an 18-year-old male with lytic rib lesions intractable pleural effusion that responded dramatically to the combination of mammalian target rapamycin (mTOR) inhibitor sirolimus aminobisphosphonate zoledronic acid after failing interferon therapy. This tolerable therapeutic has...
Glycosite-specific antibody‒drug conjugatess (gsADCs), harnessing Asn297 N-glycan of IgG Fc as the conjugation site for drug payloads, usually require multi-step glycoengineering with two or more enzymes, which limits substrate diversification and complicates preparation process. Herein, we report a series novel disaccharide-based substrates, reprogram to one-step synthesis gsADCs, catalyzed by an endo-N-acetylglucosaminidase (ENGase) Endo-S2. via ENGases has steps: deglycosylation wild-type...
This paper proposes a new texture classification algorithm that is invariant to rotation and gray-scale transformation. First, we convert two-dimensional (2-D) images one-dimensional (1-D) signals by spiral resampling. Then, use quadrature mirror filter (QMF) bank decompose sampled into subbands. In each band, take high-order autocorrelation functions as features. Features in different bands, which form vector sequence, are then modeled hidden Markov model (BMM). During classification, the...
The potential survival benefits of adding radiotherapy to systemic therapy for esophageal cancer patients with oligometastases are unknown.In this retrospective analysis, stage IV (according the American Joint Committee on Cancer Seventh edition staging system) ≤3 metastases who underwent chemotherapy cisplatin/paclitaxel between 2012 and 2015 were identified. Patients received (CT) alone vs. concurrent chemoradiotherapy (CCRT) all metastases.Of 461 patients, 97% had squamous cell cancer....
A reversible modification strategy enables a switchable cage/decage process of proteins with an array applications for protein function research. However, general N-terminal selective strategies which present site selectivity are specifically limited. Herein, we report compatible 20 canonical amino acids at the by palladium-catalyzed cinnamylation native peptides and under biologically relevant conditions. This approach broadens substrate adaptability shows potential impact on more...
To explore the genetic alterations, pathobiological functions, and downstream molecular mediators of RBM10 in endometrial cancer (EC) cells. Targeted sequencing The Cancer Genome Atlas (TCGA) dataset analysis were performed. Following knockout (KO) or exogenous overexpression EC cell lines, biological functions underlying mechanism cells evaluated by Western blot, qRT-PCR, CCK-8, Transwell, RNA-sequencing (RNA-seq), Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analysis....
Objective Ultrasound imaging has emerged as the preferred modality for ovarian tumor screening due to its non-invasive nature and real-time dynamic capabilities. However, in many developing countries, ultrasound diagnosis remains dependent on specialist physicians, where shortage of skilled professionals relatively low accuracy manual diagnoses significantly constrain efficiency. Although deep learning achieved remarkable progress medical image segmentation recent years, existing methods...
The drug-to-antibody ratio (DAR) value and dual-drug combination greatly influence the therapeutic index of antibody-drug conjugates (ADCs). reported approaches usually require multifunctional branched linkers, a complicated technologies, or protein-protein ligation, which may incorporate multihydrophobic fragments result in low coupling efficiency. Herein, we developed facile efficient one-pot method to assemble dual-site-specific ADCs with defined DARs at both N-glycosylation site K248...
Objectives To investigate the expression and significance of programmed cell death protein 1 (PD-1) ligand-1 (PD-L1) in mucosal tissues peripheral blood patients with ulcerative colitis (UC). Methods Eighty UC were recruited from January 2021 to August 2022 Shanxi Province People’s Hospital. PD-1 PD-L1 was assessed by immunohistochemistry tissues. An enzyme-linked immunosorbent assay used measure soluble levels serum, membrane-bound forms (mPD-1), (T-helper cell) Th1 Th17, determined flow...
Abstract Strain‐promoted azide–alkyne cycloaddition using dibenzoazacyclooctyne (DBCO) is widely applied in copper‐free bioorthogonal reactions. Reported here the efficient acid‐promoted rearrangement and silver‐catalyzed amidation of DBCO, which alters its click reactivity robustly. In switched reaction, as a caged acylation reagent, enables rapid peptide/protein modification after decaging facilitated by silver catalysts, rendering site‐specific conjugation an IgG antibody Fc‐targeting peptide.
<b><i>Introduction:</i></b> Classic hemodialysis schedules present inadequate middle-molecular-weight toxin clearance due to limitations of membrane-based separation processes. Accumulation uremic retention solutes may result in specific symptoms (e.g., pruritus) and affect clinical outcome patient’s quality life. Hemoperfusion (HP) is a blood purification modality based on adsorption that can overcome such limitations, thus, it be interesting test the efficacy at...
Maize (Zea mays L.) is more sensitive to low-temperature stress in the early growth period. The study was explore response mechanism of proline during maize seed germination. varieties Xinxin 2 (low-temperature insensitive) and Damin 3307 sensitive) were chosen as test materials, setting normal temperature for germination (22 °C/10 °C, 9d), (4 °C/4 5d) recovery 4d), combined with (15 mmol·L−1) soaking treatment, its effects on osmotic regulation system antioxidant protection embryos....
The inadequate understanding of the structure–activity relationship (SAR) glycosite-specific antibody–drug conjugates (ADCs) hinders its design and development. Herein, we revealed systemic SAR structure–toxicity (STR) gsADCs by constructing 50 gsADC structures bearing three glycan subtypes diverse linker-drug combinations. According to results, extra hydrophilic linkers are indispensable for intact glycan-based achieve better in vivo efficacy. Meanwhile, that conjugate complexes onto...