A5014965460- Insect symbiosis and bacterial influences
- Entomopathogenic Microorganisms in Pest Control
- Nematode management and characterization studies
- Bacterial Infections and Vaccines
- Legume Nitrogen Fixing Symbiosis
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Insect Resistance and Genetics
- Insect and Arachnid Ecology and Behavior
University of Wisconsin–Madison
2017-2022
In host-associated bacteria, surface and secreted proteins mediate acquisition of nutrients, interactions with host cells, specificity tissue localization. Gram-negative the mechanism by which many cross and/or become tethered to outer membrane remains unclear. The domain unknown function 560 (DUF560) occurs in throughout Proteobacteria has been implicated host-bacterium lipoprotein exposure. We used sequence similarity networking reveal three subfamilies DUF560 homologs. One subfamily...
Xenorhabdus innexi is a bacterial symbiont of Steinernema scapterisci nematodes, which cricket-specialist parasite and together the nematode bacteria infect kill crickets. Curiously, X. expresses potent extracellular mosquitocidal toxin activity in culture supernatants. We sequenced draft genome compared it to genomes related pathogens elucidate nature specialization. Using green fluorescent protein-expressing we confirm previous reports using culture-dependent techniques that colonizes its...
The only known required component of the newly described Type XI secretion system (TXISS) is an outer membrane protein (OMP) DUF560 family. TXISSOMPs are broadly distributed across proteobacteria, but properties cargo proteins they secrete largely unexplored. We report biophysical, histochemical, and phenotypic evidence that Xenorhabdus nematophila NilC surface exposed. Biophysical data structure predictions indicate a two-domain with C-terminal, 8-stranded β-barrel. This has been noted as...
Abstract In host-associated bacteria, surface and secreted proteins mediate acquisition of nutrients, interactions with host cells, specificity tissue-localization. Gram-negative the mechanism by which many cross or become tethered to outer membrane remains unclear. The d omain u nknown function (DUF)560 occurs in throughout Proteobacteria has been implicated host-bacteria lipoprotein exposure. We used sequence similarity networking reveal three subfamilies DUF560 homologs. One subfamily...