A5102817931- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Nerve injury and regeneration
- Cancer, Hypoxia, and Metabolism
- Nuclear Receptors and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Cancer Genomics and Diagnostics
- PI3K/AKT/mTOR signaling in cancer
- Traumatic Brain Injury and Neurovascular Disturbances
- Mesenchymal stem cell research
- Axon Guidance and Neuronal Signaling
- Glioma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Plant Genetic and Mutation Studies
- Molecular Biology Techniques and Applications
- Transgenic Plants and Applications
- Extracellular vesicles in disease
- Anesthesia and Neurotoxicity Research
- Peroxisome Proliferator-Activated Receptors
Nantong University
2015-2024
Affiliated Hospital of Nantong University
2024
Yancheng Third People's Hospital
2024
Chongqing Medical University
2024
Ministry of Education of the People's Republic of China
2021-2022
Yangtze University
2019
Liaocheng People's Hospital
2018
Xinjiang Normal University
2013-2014
Merck & Co., Inc., Rahway, NJ, USA (United States)
2012
Creighton University
2006-2009
Abstract Aberrant signaling through G-protein coupled receptors promotes metastasis, the major cause of breast cancer death. We identified regulator 4 (RGS4) as a novel suppressor migration and invasion, important steps metastatic cascades. By blocking signals initiated Gi-coupled receptors, such protease-activated receptor 1 CXC chemokine 4, RGS4 disrupted Rac1-dependent lamellipodia formation, key step involved in invasion. has GTPase-activating protein (GAP) activity, which inhibits by...
Lycium barbarum is used both as a food additive and medicinal herb in many countries, L. polysaccharides (LBPs), major cell component, are reported to have wide range of beneficial effects including neuroprotection, anti-aging anticancer properties, immune modulation. The LBPs on neuronal function, neurogenesis, drug-induced learning memory deficits not been assessed. We report the therapeutic neurogenesis scopolamine (SCO)-treated rats. were administered via gastric perfusion for 2 weeks...
Cell-free DNA (cfDNA) circulating in blood is currently used for noninvasive diagnostic and prognostic tests. Minimizing background vital detection of low abundance cfDNA. We investigated whether a new collection device could reduce levels genomic (gDNA) plasma compared to K(3) EDTA tubes, when subjected conditions that may occur during sample storage shipping.Blood samples were drawn from healthy donors into Cell-Free DNA™ BCT (BCT). To simulate shipping, shaken or left unshaken. In...
The enumeration and characterization of circulating tumor cells (CTCs) in the blood cancer patients is useful for prognostic treatment monitoring purposes. number CTCs present patient very low; thus, robust technologies have been developed to enumerate characterize samples. One challenges clinical utility their inherent fragility, which makes these unstable during transportation storage In this study we investigated Cell-Free DNA BCT™ (BCT), a collection device, stabilizes sample at room...
The therapeutic potential of umbilical cord blood mesenchymal stem cells has been studied in several diseases. However, the possibility that human Wharton's jelly-derived (hUCMSCs) can be used to treat neonatal hypoxic-ischemic encephalopathy (HIE) not yet investigated. This study focuses on effect hUCMSC transplantation a rat model HIE. Dermal fibroblasts served as cell controls. HIE was induced rats aged 7 days. hUCMSCs labeled with Dil were then transplanted into models 24 hr or 72...
Our previous studies indicated that transcription factor Brn-4 is upregulated in the surgically denervated hippocampus vivo, promoting neuronal differentiation of hippocampal neural stem cells (NSCs) vitro. The molecules mediating upregulation remain unknown. In this study we examined levels insulin-like growth factor-1 (IGF-1) following denervation. Surgical denervation led to a significant increase IGF-1 expression vivo. We also report treatment on NSCs vitro marked acceleration and cell...
Cell-free RNA (cfRNA) naturally occurs in blood and has clinical significance. Accurate quantification of these extracellular RNAs whole is hindered by the simultaneous unintended release cellular degradation cfRNA after draw. An appropriate collection device needed to stabilize during processing, transportation storage, which will ensure test reliability. In this study we compared a novel against traditional K3EDTA tubes for its ability when subjected conditions that can occur sample...
CXCL12 and its physiologic receptor CXCR4 are involved in controlling cell survival, proliferation migration adult tissues. This study aimed to investigate the effects of on cortical neuron apoptosis rats after traumatic brain injury (TBI) potential mechanisms involved. At 3 days TBI, situ terminal transferase d-UTP nick-end labeling assay (TUNEL) showed that apoptotic index (AI) deceased significantly treatment group compared with control (p < 0.05). Immunofluorescence double-labeled...
To investigate whether a new blood collection device stabilizes cell-free RNA (cfRNA) in post-phlebotomy when compared to using K3EDTA tubes. Blood samples were drawn from healthy donors into tubes and Cell-Free BCTs (BCTs) stored at room temperature (20–25 °C). At specified time points (days 0–3), plasma was separated cfRNA extracted. Reverse transcription real-time PCR used quantify mRNA for c-fos, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S rRNA. showed steady increase...
// Xiaoqing Yang 1 , Xinhua Zhang 2 Rongrong Wu Qicheng Huang Yao Jiang Jianbing Qin Feng Guohua Jin Yuquan Department of Obstetrics and Gynecology, Affiliated Hospital Nantong University, NanTong, Jiangsu 226006, People's Republic China Anatomy, Nantong, JiangSu 226000, Correspondence to: Zhang, email: jsnt_zhangyuquan@163.com Jin, jguohua@ntu.edu.cn Keywords: endometrial carcinoma, dipeptidyl peptidase IV, sitagliptin, hypoxia-inducible factor 1a, vascular endothelial growth A Received:...
The regulation of adult neural stem cells (NSCs) is critical for lifelong neurogenesis. MicroRNAs (miRNAs) are a type small, endogenous RNAs that regulate gene expression post-transcriptionally and influence signaling networks responsible several cellular processes. In this study, miR-103-3p was transfected into derived from embryonic hippocampal cells. results showed suppressed cell proliferation differentiation, promoted apoptosis. addition, negatively regulated NudE neurodevelopment...
Focal and diffuse neuronal loss happened after traumatic brain injury (TBI). With little in the way of effective repair, recent interest has focused on endogenic neural progenitor cells (NPCs) as a potential method for regeneration. Whether regeneration cortex adult rat TBI remains to be determined. In this study, rats were divided into sham group group, model medium was induced by controlled cortical impact. Rats injected with BrdU at 1 7 days post-injury (dpi) allow identification...
Resveratrol is one of the most studied plant secondary metabolites owing to its numerous health benefits. It accumulated in some plants following biotic and abiotic stress pressures, including UV-C irradiation. Polygonum cuspidatum represents major natural source concentrated resveratrol but underlying mechanisms as well effects irradiation on content have not yet been documented. Herein, we found that significantly increased by 2.6-fold 1.6-fold irradiated leaf samples followed a dark...
Human Wharton's jelly-derived mesenchymal stem cells (hWJ-MSCs) have excellent proliferative ability, differentiation low immunogenicity, and can be easily obtained. However, there are few studies on their application in the treatment of ischemic stroke, therefore therapeutic effect requires further verification. In this study, hWJ-MSCs were transplanted into an stroke rat model via tail vein 48 hours after transient middle cerebral artery occlusion. After 4 weeks, neurological functions...
Neural stem cells (NSCs) persist in the dentate gyrus of hippocampus into adulthood and are essential for both neurogenesis neural circuit integration. Exosomes have also been shown to play vital roles regulating biological processes receptor as a medium cell-to-cell communication signaling molecules. The precise molecular mechanisms exosome-mediated signaling, however, remain largely unknown. Here, we found that exosomes produced by denervated hippocampi following fimbria-fornix transection...
Neurogenesis in the hippocampus continues throughout adult life and can be regulated by local microenvironment. To determine whether denervation stimulates neurogenesis hippocampus, proliferation, migration, differentiation of neural stem cells (NSCs) dentate gyrus was investigated after fimbria fornix transection. In denervated NSCs proliferated markedly migrated along subgranular layer, more newborn differentiated into neurons or astrocytes. After denervation, deafferented expressed Brn-4...
The denervated hippocampus provides a proper microenvironment for the survival and neuronal differentiation of neural progenitors. While thousands lncRNAs were identified, only few that regulate neurogenesis in are reported. present study aimed to perform microarray expression profiling identify long noncoding RNAs (lncRNAs) might participate hippocampal neurogenesis, investigate potential roles identified neurogenesis. In this study, suggested 74 activated 29 repressed (|log...
Abstract Neural stem cells (NSCs) possess self‐renewal and multilineage differentiation ability, thus are considered to be a potential source for cell replacement therapy of many nervous system diseases, such as neurodegenerative diseases. Valproate (VPA), member histone deacetylase inhibitor family, is an epigenetic regulator can promote NSCs differentiate into neurons, nevertheless, the underlying mechanisms process remain unclear. MicroRNAs (miRNAs) exert crucial part in...