A5016303261- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Enzyme Catalysis and Immobilization
- CRISPR and Genetic Engineering
- Algal biology and biofuel production
- DNA Repair Mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Plant biochemistry and biosynthesis
- RNA and protein synthesis mechanisms
- Fermentation and Sensory Analysis
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Microbial bioremediation and biosurfactants
- biodegradable polymer synthesis and properties
- RNA modifications and cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Metabolism and Applications
- Biochemical and biochemical processes
- Enzyme Structure and Function
- Metabolomics and Mass Spectrometry Studies
- Protein Hydrolysis and Bioactive Peptides
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Studies on Chitinases and Chitosanases
Tianjin Institute of Industrial Biotechnology
2016-2025
Chinese Academy of Sciences
2016-2025
University of North Carolina at Chapel Hill
2021-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2024
Qilu Hospital of Shandong University
2023
Harbin Engineering University
2023
University of Chinese Academy of Sciences
2020-2022
Tianjin Economic-Technological Development Area
2022
Shandong University
2022
Intelligent Synthetic Biology Center
2022
Starches, a storage form of carbohydrates, are major source calories in the human diet and primary feedstock for bioindustry. We report chemical-biochemical hybrid pathway starch synthesis from carbon dioxide (CO2) hydrogen cell-free system. The artificial anabolic (ASAP), consisting 11 core reactions, was drafted by computational design, established through modular assembly substitution, optimized protein engineering three bottleneck-associated enzymes. In chemoenzymatic system with spatial...
Abstract Oncogene-induced replication stress generates endogenous DNA damage that activates cGAS–STING-mediated signalling and tumour suppression 1–3 . However, the precise mechanism of cGAS activation by remains enigmatic, particularly given high-affinity histone acidic patch (AP) binding constitutively inhibits sterically hindering its double-stranded (dsDNA) 4–10 Here we report double-strand break sensor MRE11 suppresses mammary tumorigenesis through a pivotal role in regulating...
Rhamnolipid as a potent natural biosurfactant has wide range of potential applications, including enhanced oil recovery (EOR), biodegradation, and bioremediation. is composed rhamnose sugar molecule beta-hydroxyalkanoic acid. The rhamnosyltransferase 1 complex (RhlAB) the key enzyme responsible for transferring moiety to acid biosynthesize rhamnolipid. Through transposome-mediated chromosome integration, RhlAB gene was inserted into Pseudomonas aeruginosa PAO1-rhlA(-) Escherichia coli BL21...
The highest titer of the anticancer precursor sesquiterpene germacrene A was observed in oleaginous yeast using multi-layered systematic metabolic engineering strategies.
The methyltransferase DOT1L methylates histone H3 at K79 to facilitate specific biological events. H3K79 dimethylation (H3K79‐2Me) by influences the DNA damage response promoting 53BP1 recruitment sites; however, it is unclear if this methylation required as interacts with dimethylated H4 (H4K20‐2Me) a much higher affinity. We demonstrate that H3K79‐2Me, while negligible during S‐phase, for ionizing radiation (IR)‐induced foci formation G1/G2‐phases when H4K20‐2Me levels are low. Further, we...
Abstract Recent reports have demonstrated that DNA double-strand break (DSB)–induced small RNAs (diRNA) play an important role in the damage response (DDR). However, molecular mechanism by which diRNAs regulate DDR remains unclear. Here, we report Dicer- and Drosha-dependent function as guiding molecules to promote recruitment of methyltransferase MMSET (WHSC1) acetyltransferase Tip60 (KAT5) DSB, where local levels histone H4 di- tri-methylation at lysine 20 (H4K20me2, 3) acetylation 16...
Lipases are ubiquitous enzymes of great physiological significance that have been used extensively in multiple industries.
Research accumulated over the past decades has shown that mycoprotein could serve as a healthy and safe alternative protein source, offering viable substitute for animal- plant-derived proteins. This study evaluated impact of substituting whey with fungal-derived at different levels (10%, 20%, 30%) on quality high-protein nutrition bars (HPNBs). It focused nutritional content, textural changes storage, sensory properties. Initially, all displayed similar hardness, but storage time...
Saccharomyces cerevisiae has been intensively studied in responses to different environmental stresses such as heat shock through global omic analysis. However, the S. industrial strains with superior thermotolerance have not explored any proteomic studies for elucidating tolerance mechanism. Recently a new diploid strain was obtained evolutionary engineering of parental strain, and it exhibited even higher resistance prolonged thermal stress. Herein, we performed iTRAQ-based quantitative...
Microbial biofuel synthesis attracting increasing attention. Great advances have been made in producing fatty alcohols from acyl-CoAs and acids Escherichia coli. However, the low titers limited knowledge regarding basic characteristics of alcohols, such as location toxicity, hampered large-scale industrialization. Further research is still needed.In this study, we designed a novel efficient strategy to enhance alcohol production by inducing acid starvation. We report first use deletions...
High-temperature fermentation is desirable for the industrial production of ethanol, which requires thermotolerant yeast strains. However, thermotolerance a complicated quantitative trait. The understanding genetic basis behind high-temperature performance still limited. Quantitative trait locus (QTL) mapping by pooled-segregant whole genome sequencing has been proved to be powerful and reliable approach identify loci, genes single nucleotide polymorphism (SNP) variants linked traits yeast....