A5102772592- Electron and X-Ray Spectroscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Lepidoptera: Biology and Taxonomy
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Pleural and Pulmonary Diseases
- Phonocardiography and Auscultation Techniques
- Forest Insect Ecology and Management
- Coleoptera Taxonomy and Distribution
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Animal Disease Management and Epidemiology
- Animal Behavior and Welfare Studies
- Ultrasound in Clinical Applications
Centro Nacional de Biotecnología
2022-2024
Sociedad Española de Medicina Interna
2024
Universidad Autónoma de Madrid
2022
Image processing in cryogenic electron tomography (cryoET) is currently at a similar state as Single Particle Analysis (SPA) microscopy (cryoEM) was few years ago. Its data workflows are far from being well defined and the user experience still not smooth. Moreover, file formats of different software packages their associated metadata standardized, mainly since developed by groups, focusing on steps pipeline. The Scipion framework, originally for SPA (de la Rosa-Trevín et al., 2016), has...
Abstract Image-processing pipelines require the design of complex workflows combining many different steps that bring raw acquired data to a final result with biological meaning. In image-processing domain cryo-electron microscopy single-particle analysis (cryo-EM SPA), hundreds must be performed obtain three-dimensional structure macromolecule by integrating spread over thousands micrographs containing millions copies allegedly same macromolecule. The execution such complicated demands...
Regular health monitoring is crucial in laboratory animal facilities to determine the presence or absence of specific pathogens. One common approach involves use sentinel animals, which are periodically exposed biological material from cages being monitored. At a certain point, some these animals tested for This article discusses designing an effective sampling scheme meet desired quality standards. It addresses questions such as number required, frequency material, selection based on...
A progressive CryoEM map validation scheme is presented. The may include the map, half maps, 2D classes, particles, angles, coordinates, defoci, micrographs, and image processing workflow.