Introduction: Brazil is the world’s largest exporter of chicken meat. Poultry and poultry products are often associated with non-typhoidal Salmonella (NTS) contamination. Reports on antimicrobial-resistant (AMR) NTS serovars in isolates from Brazilian farms concerning. In response, interventions implemented chains to remove microbial contaminants, but they may also exert selective bacterial pressure. Objective: To characterize adaptation mechanisms occurring throughout meat processing...

Abstract The activity of pectic enzymes was measured in culture filtrates four selected fungal strains. Pectin lyase (PL) produced by all fungi, but pectinesterase not detected the strain Penicillium expansum F16. use crude resulted good clarification apple juice as evaluated an increase transmittance at 660 nm. PL activities were partially purified dye-affinity chromatography on Blue Sepharose. enzyme bound to column and eluted a single peak, free PE activity, coinciding with major amount...

Little information about Shigella responsible for foodborne shigellosis is available in Brazil. The present study aimed to investigate the antimicrobial resistance and PCR-ribotyping patterns of isolates outbreaks occurred Rio Grande do Sul State (RS), Southern Brazil period between 2003 2007. strains (n=152) were isolated from foods fecal samples victims investigated by Surveillance Service. Identification at specie level indicated that 71.1% them S. flexneri, 21.5% sonnei, 0.7%...

Bacterial contamination remains the major problem in skin banks, even after antimicrobial treatment, and results high rates of tissue discarding. This study aimed to analyze bacterial 32 human allografts from bank Dr. Roberto Corrêa Chem Hospital Complex Santa Casa de Misericórdia Porto Alegre. These samples were already discarded due microbial contamination. The identification bacteria isolated was performed by matrix assisted laser desorption ionization–time flight. susceptibility isolates...

Introdução: O transplante de córneas é o principal tratamento para pessoas que apresentam distúrbios curvatura ou transparência da córnea. No Brasil, não há protocolo unificado meios preservação, tempo armazenamento e antibióticos utilizados. A preocupação a patógenos possam ser transferidos aos receptores transplantes. Objetivo: Realizar levantamento microbiota ocular doadores fim verificar uma possível correlação com infecções em e, dessa forma, auxiliar na melhoria metodologias protocolos...

Cellulose-hydrolysing enzymes from the phytopathogenic fungus Bipolaris sorokiniana were partially purified and characterised. The enzyme production was variable according to carbon source. β-Glucosidase cellobiohydrolase activities higher by growing on cellulose than other sources. Carboxymethyl cellulase stimulated carbohydrates, mainly lactose. Partial purification carried out liquid chromatography Sepharose CL4B. about 17-fold, with a yield of 41% as judged assay...

ABSTRACT Antimicrobial resistance was determined for 73 isolates of Salmonella enteritidis isolated from foods involved in human foodborne outbreaks that occurred the South Brazil, 1999 to 2000. The were individually tested against 10 antimicrobial agents using a disc diffusion method. Most susceptible all drugs tested. No S. resistant sulfamethoxazole/trimethoprim or chloramphenicol. predominant observed streptomycin (S) (37%), gentamicin (GEN) (13.7%) and nalidixic acid (NAL) (13.7%),...

In September 2005, the Sanitary Surveillance Service of Rio de Janeiro (SSS/RJ), Brazil, investigated a case gastroenteritis involving 13-year-old teenager hospitalized because bloody diarrhea and severe abdominal pain. Owing to severity symptoms, an epidemiological investigation was conducted in two states Brazil. Escherichia coli O157:NM isolated from stools tomato cheese salad prepared at school canteen where attended. This is first report human related E. infection

N-acetylhexosaminidase (HEX) from the phytopathogenic fungus Bipolaris sorokiniana was isolated and characterized. The production of HEX by B. not altered growing on different carbon sources. Enzyme purification carried out sequential liquid chromatography Sephacryl S-200 HR, p-aminobenzyl-2-acetamido-2-deoxy-β-d-thioglucopyranoside agarose. about 70-fold, with a yield 41%, determined p-nitrophenyl-N-acetylglucosaminide as substrate. enzyme had pH temperature optima 4·5 55 °C, respectively....

Several enzymatic activities were investigated in six isolates of the fungus Bipolaris sorokiniana, originating from different areas Brazil. Among glycosidases studied, beta-glucosidase, beta-N-acetylglucosaminidase, beta-xylosidase, cellobiohydrolase, and chitobiohydrolase major activities. In some isolates, beta-glucuronidase, beta-galactosidase, alpha-mannosidase also present. Polysaccharide-hydrolyzing enzymes, such as pectin lyase carboxymethyl cellulase detected significant amounts,...

Some biological effects of gamma-rays have been evaluated in cells the bacteria Escherichia coli, through comparison number colonies formed by a unitary cell (CFU) proceeded from an unirradiated control suspension and irradiated suspension, visualization possible damage microorganism enzymatic system due to irradiation with high energy photons (/sup 60/Co /spl gamma/-rays). For this purpose, for absorbed doses at up 280 Gy, methods successive dilutions sowing Petri plates containing simple...