A5018042617- Endometrial and Cervical Cancer Treatments
- Pregnancy and preeclampsia studies
- Ovarian cancer diagnosis and treatment
- Cervical Cancer and HPV Research
- Menopause: Health Impacts and Treatments
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Prenatal Screening and Diagnostics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- Uterine Myomas and Treatments
- Cancer Cells and Metastasis
- Reproductive System and Pregnancy
- Estrogen and related hormone effects
- Air Quality and Health Impacts
- Maternal and fetal healthcare
- Endometriosis Research and Treatment
- Birth, Development, and Health
- Phytoestrogen effects and research
- Neuroscience of respiration and sleep
- Infant Development and Preterm Care
- Protein Kinase Regulation and GTPase Signaling
- Genetic Syndromes and Imprinting
- Neonatal and fetal brain pathology
Kyushu University
2016-2025
Kyushu University Hospital
2014-2025
Tokyo Medical and Dental University
2014-2023
Nihon University
2020-2023
Osaka University
2008-2023
University of Occupational and Environmental Health Japan
2023
Tohoku University
2023
Keio University
2023
Yamagata University Hospital
2023
Tottori University
2023
We have introduced a variety of amino acid substitutions into carboxyl-terminal CA1A2X sequence (C = cysteine; A aliphatic; X any acid) the oncogenic [Val12]Ki-Ras4B protein to identify acids that permit Ras processing (isoprenylation, proteolysis, and carboxyl methylation), membrane association, transformation in cultured mammalian cells. While all were tolerated at A1 position, A2 reduced transforming activity. The residue was important for both isoprenylation AAX whereas dictated extent...
It has been proposed that the human endometrium may contain a population of adult stem cells are responsible for its remarkable regenerative capability. Recently, subset or progenitor in tissue identified as side-population (SP cells) displaying low staining with Hoechst 33342 by fluorescence-activated cell sorter (FACS) analysis. In this study, we isolated SP from and analysed their properties.Endometrial were obtained using enzymatic digestion uterine hysterectomy treatment myoma stained...
Clear cell carcinoma (CCC) is a rare histologic subtype that demonstrates poor outcomes in epithelial ovarian cancer. The Japanese Gynecologic Oncology Group conducted the first randomized phase III, CCC-specific clinical trial compared irinotecan and cisplatin (CPT-P) with paclitaxel plus carboplatin (TC) patients CCC.Six hundred sixty-seven stage I to IV CCC of ovary were randomly assigned receive 60 mg/m(2) on days 1, 8, 15 day 1 (CPT-P group) every 4 weeks for six cycles or 175 area...
Clear cell carcinoma of the uterine cervix is a rare subtype. We report case 14-year-old girl who presented with abnormal genital bleeding, 7 cm mass arising from cervix, and swollen right inguinal lymph nodes. Histological immunohistochemical examinations showed clear carcinoma. performed modified-radical hysterectomy, bilateral salpingo-oophorectomy, node biopsy, pelvic para-aortic diagnosed cervical cancer stage IVB (clear carcinoma, pT2bN2M1). administered...
Loss of genomic imprinting is involved in a number developmental abnormalities and cancers. ZAC an imprinted gene expressed from the paternal allele chromosome 6q24 within region known to harbor tumor suppressor for several types neoplasia. p57 KIP2 ( CDKN1C ) maternally located on 11p15.5 which encodes cyclin-dependent kinase inhibitor that may also act as gene. Mutations have been implicated transient neonatal diabetes mellitus (TNDB) Beckwith–Wiedemann syndrome, respectively. Patients...
Abstract The microtubule associated protein tau is a major component of neurofibrillary tangles in Alzheimer disease brain, however the neuropathological processes behind formation are still unclear. Previously, 14‐3‐3 proteins were reported to bind with tau. Proteins usually their targets through specific serine/threonine –phosphorylated motifs. Therefore, interaction mediated by phosphorylation was investigated. In this study, we show that either kinase A (PKA) or B (PKB) enhances binding...
This study aims to assess the effects of proanthocyanidin-a class polyphenol antioxidants extracted from grape seeds-on menopausal symptoms, body composition, and cardiovascular parameters in middle-aged women.We conducted a randomized, double-blind, placebo-controlled 96 women aged 40 60 years who had at least one symptom. The participants were randomized receive seed extract tablets containing either low-dose (100 mg/d) or high-dose (200 proanthocyanidin, placebo, for 8 weeks. Their...
This study aimed to investigate the effects of grape seed proanthocyanidin extract (GSPE) on blood pressure and vascular endothelial function in middle-aged Japanese adults with prehypertension. We conducted a randomized, double-blind, placebo-controlled 6 men 24 women aged 40–64 years old. The participants were randomized receive tablets containing either low-dose (200 mg/day) or high-dose (400 GSPE, placebo, for 12 weeks. Systolic diastolic pressures (SBP DBP, respectively), brachial...
Although the Rap1A protein resembles oncogenic Ras proteins both structurally and biochemically, exhibits no properties. Rather, overexpression of can reverse Ras-induced transformation NIH 3T3 cells. Because greatest divergence in amino acid sequence between occurs at COOH terminus, role this domain opposing biological activities these was examined. COOH-terminal processing membrane association were studied by constructing expressing a chimeric (composed residues 1 to 110 an H-Ras activated...
Recently, numerous studies have identified that immature cell populations including stem cells and progenitor can be found among "side-population" (SP) cells. Although SP isolated from some adult tissues been reported elsewhere, isolation characterization of human trophoblast remained to reported. In this study, HTR-8/SVneo primary villous cytotrophoblasts (vCTBs) were stained with Hoechst 33342 non-SP (NSP) fractions using a sorter. A small population was in vCTBs. expressed several...
Many studies have demonstrated the effectiveness of isoflavones on menopausal symptoms; however, these mostly used high dosages. Because high-dose isoflavone may result in endometrial hyperplasia, we investigated whether low-dose aglycone alleviates symptoms similarly to dosages.We conducted a randomized, double-blind, placebo-controlled study 90 healthy women aged 40-60 years who had at least one symptom Menopausal Symptom Scale (MSS). The participants were randomized receive active tablets...
Alzheimer's disease (AD) is a progressive neurodegenerative disorder clinically characterized by loss of memory and cognition. The effective therapeutic options for AD are limited thus there demand new drugs. Aegle marmelos (Linn.) (A. marmelos) leaves have been used in traditional medicine to promote intellect enhance memory. In this study, we evaluated A. its acetylcholinesterase (AChE) inhibitory activity antioxidant property vitro the treatment AD.A crude methanol extract four fractions...
<h3>Objective</h3> To assess the short-term prognosis of Japanese infants with a birth weight (BW) ≤500 g. <h3>Study design</h3> Demographic and clinical data were reviewed for 1473 live born BW g at gestational age ≥22 weeks who treated in 204 affiliated hospitals Neonatal Research Network Japan between 2003 2012. <h3>Results</h3> Survival to hospital discharge occurred 811 (55%; 95% CI 53%-58%). The survival rates ≤300 g, 301-400 401-500 18% (95% 10%-31%), 41% 36%-47%), 60% 57%-63%),...
The Ras(17N) dominant negative antagonizes endogenous Ras function by forming stable, inactive complexes with guanine nucleotide exchange factors (GEFs; e.g., SOS1). We have used the growth-inhibitory phenotype of to characterize two aspects interaction GEFs. First, we a nonprenylated version Ras(17N), designated Ras(17N/186S), which no longer associates plasma membrane and lacks phenotype, address importance subcellular location posttranslational modification for its observed that addition...
We demonstrated here the growth-suppressing effects of sodium butyrate (NaB) on human endometrial and ovarian cancer cells. The arrest cells at G1 checkpoint accounted for this effect. NaB-mediated p21 might G0/G1 phase by eliciting pRb unphosphorylation. To demonstrate role regulation p21, we measured sensitivity to NaB cervical in which had been inactivated HPV E7. displayed a G2/M addition their arrest. Arrest accompanied induction senescence-like phenotypes (SLPs). Most importantly,...
Abstract ZAC is a paternally expressed, imprinted gene located on chromosome 6q24, within region known to harbor tumor suppressor for several types of neoplasia, including human ovarian cancer (HOC). We have failed identify genetic mutations in the material. Many genes contain differentially allele‐specific‐methylated regions (DMR) and promoter activity that regulated by DNA methylation. Aberrant methylation common feature neoplasia changes at locus been reported some cases HOC. investigated...