A5053356628- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- COVID-19 diagnosis using AI
Dalhousie University
2020-2021
We demonstrate the application of a rapid pre-concentration protocol and magnetic bead-based RNA extraction method for detection SARS-CoV-2 from raw wastewater without need extensive sample treatment.
Abstract The use of magnetic beads in the extraction nucleic acids from wastewater is presented as an approach to simplify techniques for detection SARS-CoV-2 viral fragments wastewater. In particular, this paper describes development and optimization a direct method extracting RNA municipal using beads. recovery efficiency Accuplex Positive Reference Material (ASCV-2) was examined. Method factors assessed were sample volume, concentration bead mix, elution temperature, water matrix...