Naofumi Shiomi

ORCID: 0000-0003-0079-2485
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Research Areas
  • Radiation Therapy and Dosimetry
  • Microbial Metabolites in Food Biotechnology
  • Electron and X-Ray Spectroscopy Techniques
  • Polyamine Metabolism and Applications
  • Atomic and Molecular Physics
  • Adipose Tissue and Metabolism
  • Monoclonal and Polyclonal Antibodies Research
  • Biopolymer Synthesis and Applications
  • Fungal and yeast genetics research
  • X-ray Spectroscopy and Fluorescence Analysis
  • Protein purification and stability
  • Nuclear Physics and Applications
  • Adipokines, Inflammation, and Metabolic Diseases
  • Viral Infectious Diseases and Gene Expression in Insects
  • Enzyme Structure and Function
  • Polysaccharides and Plant Cell Walls
  • Glycosylation and Glycoproteins Research
  • Plant nutrient uptake and metabolism
  • Nuclear physics research studies
  • Biofuel production and bioconversion
  • Enzyme Production and Characterization
  • Enzyme Catalysis and Immobilization
  • Amino Acid Enzymes and Metabolism
  • DNA Repair Mechanisms
  • Extraction and Separation Processes

Kobe University
2024-2025

Kobe College
2006-2019

National Institutes for Quantum Science and Technology
2013

Rakuno Gakuen University
1992-2010

Kaneka (Japan)
1995-1997

Takasago (Japan)
1988-1997

Kaneka (United States)
1990-1995

Hokkaido University
1992

Takasago (United States)
1990

Nara Women's University
1974-1986

ABSTRACT Complementation analysis of a polyhydroxyalkanoate (PHA)-negative mutant Aeromonas caviae proved that ORF3 in the pha locus (a 402-bp gene located downstream PHA synthase gene) participates biosynthesis on alkanoic acids, and is here referred to as phaJ Ac . Escherichia coli BL21(DE3) carrying under control T7 promoter overexpressed enoyl coenzyme A (enoyl-CoA) hydratase, which was purified by one-step anion-exchange chromatography. The N-terminal amino acid sequence hydratase...

10.1128/jb.180.3.667-673.1998 article EN Journal of Bacteriology 1998-02-01

Switching from a replicative to translesion polymerase is an important step further continue on replication at the site of DNA lesion. Recently, RAD18 (a ubiquitin ligase) was shown monoubiquitinate proliferating cell nuclear antigen (PCNA) in cooperation with RAD6 ubiquitin-conjugating enzyme) replication-stalled sites, causing switch. Analyzing -knockout (RAD18−/−) cells generated human HCT116 cells, addition switch, we found new function for S phase-specific single-strand break repair...

10.1093/nar/gkl979 article EN cc-by-nc Nucleic Acids Research 2006-12-07

Energy losses of 7-MeV protons in metallic and organic thin foils have been obtained as a function emergence angles. Angular distributions due to multiple scattering also measured. The energy loss is found increase for all targets with increasing the cannot be explained by following three effects: (1) target thickness caused deflection particle, (2) path length atomic nuclei, (3) transfer recoil nuclei during multiple-scattering process. From this consideration, observed angle concluded...

10.1103/physreva.25.2524 article EN Physical review. A, General physics 1982-05-01

summary Contents of fructo‐oligosaccharides and fructo‐polysaccharides activities fructosyltransferases invertase in asparagus roots harvested between July November were studied by gel permeation chromatography (GPC) high performance liquid (HPLC). total sugar very low July, then increased until September. During this period, sucrose accumulated first, second trisaccharides, tetra‐, penta‐ higher‐saccharides that order. 1‐Kestose was the most predominant fructo‐ohgosacchande isomer [degree...

10.1111/j.1469-8137.1992.tb00069.x article EN New Phytologist 1992-11-01

<title>Abstract</title> Purpose For high-density cell culture, we studied the development of optimal microfibers (MFs) with a 0.1–10 µm diameter, which due to their large surface area can serve as an immobilization carrier for animal cells. To date, few studies have used MFs scaffolding culturing. Results Using six types nonsoluble synthetic polymers, MF sheets were fabricated by electrospinning. The cellulose acetate, polyketone, and polyvinyl acetate exhibited swelling water retention...

10.21203/rs.3.rs-5921426/v1 preprint EN cc-by Research Square (Research Square) 2025-03-25

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolubility of carbon dioxide in molten alkali halides and nitrates their binary mixturesEizo Sada, Shigeo Katoh, Hidehumi Yoshii, Ikuo Takemoto, Naofumi ShiomiCite this: J. Chem. Eng. Data 1981, 26, 3, 279–281Publication Date (Print):July 1, 1981Publication History Published online1 May 2002Published inissue 1 July 1981https://pubs.acs.org/doi/10.1021/je00025a017https://doi.org/10.1021/je00025a017research-articleACS PublicationsRequest reuse...

10.1021/je00025a017 article EN Journal of Chemical & Engineering Data 1981-07-01

10.1016/0029-554x(82)90490-6 article EN Nuclear Instruments and Methods in Physics Research 1982-03-01

Onion and shallot (Allium cepa L.) exhibit wide variation in bulb fructan content, the Frc locus on chromosome 8 conditions much of this variation. To understand biochemical basis Frc, we conducted genetic analyses Allium fistulosum (FF)–shallot (A. Aggregatum group) alien monosomic addition lines (AALs; FF+1A–FF+8A) onion mapping populations. Sucrose levels leaves FF+2A were significantly lower than FF throughout year, springtime activity acid invertase was also lower. FF+8A showed higher...

10.1093/pcp/pcn048 article EN Plant and Cell Physiology 2008-03-11

Purpose: Telomere length (TL) is an indicator of age; however, hormonal influences complicate individual aging. It remains unclear whether TL shortening a direct factor in both and cellular Therefore, we examined the relationship between senescence at level. Methods: Telomerase activity, TL, gene expression were measured cultured human lung-, fetal-, skin-derived fibroblasts, skin keratinocytes, telomerase reverse transcriptase (TERT) gene-immortalized cells using detection kits, Cawthon's...

10.4236/jbm.2024.127006 article EN Journal of Biosciences and Medicines 2024-01-01

The relationship between the expression of HLA-DR antigens and alpha gene methylation was examined in systemic lupus erythematosus (SLE). Using permanent B cell lines, we found reduced DR SLE. low correlated with high anti-DNA antibody titers patients' sera. amounts message were lower SLE cells than normal controls, suggesting that is associated functions. extent DNA at five CCGG sites locus. from both showed variable patterns. Since a single-copy gene, such variability result assaying...

10.1172/jci112105 article EN Journal of Clinical Investigation 1985-10-01

The emergence-angle dependence of energy loss 7-MeV protons in Cu foils, the thicknesses which are 3.743 and 7.576 mg/${\mathrm{cm}}^{2}$, has been measured with very high angular resolution. It was found that both cases increases as emergence angle increases. amount increase depends strongly on thickness sample foils. Computer simulation performed order to investigate origin this phenomenon. Individual collision undergo within target be restricted by determination detection angles....

10.1103/physreva.27.810 article EN Physical review. A, General physics 1983-02-01
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