A5045462361- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Immune cells in cancer
- Heme Oxygenase-1 and Carbon Monoxide
- Histiocytic Disorders and Treatments
- Cytokine Signaling Pathways and Interactions
- Receptor Mechanisms and Signaling
- Hemoglobin structure and function
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- PARP inhibition in cancer therapy
- Calcium signaling and nucleotide metabolism
- Neonatal Health and Biochemistry
- Hedgehog Signaling Pathway Studies
Boehringer Ingelheim (Germany)
2019-2022
Medical University of Vienna
2013-2021
Institute of Immunology
2013
In many solid cancers, tumor-associated macrophages (TAM) represent the predominant myeloid cell population. Antigen (Ag) cross-presentation leading to tumor Ag-directed cytotoxic CD8+ T responses is crucial for antitumor immunity. However, role of recruited monocyte-derived macrophages, including TAM, as potential cross-presenting cells not well understood. Here, we show that primary human mouse CD206+ are effective in functional soluble self-Ag and non-self-Ag, Ag (TAA), viral Ag. To...
Human Langerhans cell (LC) precursors populate the epidermis early during prenatal development and thereafter undergo massive proliferation. The prototypic antiproliferative cytokine TGF-β1 is required for LC differentiation from human CD34+ hematopoietic progenitor cells blood monocytes in vitro. Similarly, deficiency results loss vivo. However, immunohistology studies revealed that niches adult basal (germinal) keratinocyte layers lack detectable TGF-β1. Here we demonstrated these express...
Schlafen (SLFN/Slfn) family members have been investigated for their involvement in fundamental cellular processes including growth regulation, differentiation and control of viral replication. However, most research has focused on the characterization Slfns within murine system or human cell lines. Since little is known about SLFNs primary immune cells, we set out to analyze expression regulation six SLFN genes monocytes, monocyte-derived dendritic cells (moDCs) T cells. Comparison gene...
Abstract Iron is essential for living cells. Uptake of iron-loaded transferrin by the receptor 1 (CD71, TFR) a major but not sufficient mechanism and an alternative ligand CD71 has been assumed. Here, we demonstrate that utilizes heme-albumin as cargo to transport iron into human Binding endocytosis via was promote proliferation various cell types in absence transferrin. Growth differentiation cells induced dependent on heme-oxygenase (HO-1) function accompanied with increase intracellular...
The interferon‐inducible transcription factor STAT1 is a tumor suppressor in various malignancies. We investigated sex‐specific functions colitis and colitis‐associated colorectal cancer (CRC) using mice with specific deletion intestinal epithelial cells (STAT1 ∆IEC ). Male but not female were more resistant to DSS‐induced than sex‐matched flox/flox controls displayed reduced intraepithelial infiltration of CD8 + TCRαβ granzyme B T cells. Moreover, DSS treatment failed induce expression...
Iron uptake via the transferrin receptor (CD71) is a pivotal mechanism for T cell proliferation. Yet, it incompletely understood if targeting of CD71 also affects differentiation and functional polarization primary human cells. In this study, we demonstrate that inhibition iron ingestion with blocking mAbs against induces nonproliferating cells, which release high amounts IL-2. Targeting or nonblocking did not alter major signaling pathways activation transcription factors NF-κB, NFAT, AP-1...
The cytoplasmic tail of CD45 (ct-CD45) is proteolytically cleaved and released upon activation human phagocytes. It acts on T cells as an inhibitory, cytokine-like factor in vitro. Here, we show that ct-CD45 abundant peripheral blood plasma from healthy adults compared with derived umbilical cord patients rheumatoid arthritis or systemic lupus erythematosus. Plasma depleted enhanced T-cell proliferation, while addition exogenous protein inhibited proliferation reduced cytokine production...
Background: CD97 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) receptor family and dominantly expressed on immune cells in variety malignant diseases. B7-H1 B7-H3 are proteins that involved suppression system. The aim this study was to evaluate if these molecules up-regulated patients with cancer change during chemotherapy. Materials Methods: We analyzed cluster differentiation (CD) protein expression levels tumor cell lines blood samples 37 solid tumors at...
Co-receptors, being either co-stimulatory or co-inhibitory, play a pivotal role in T-cell immunity. Several studies have indicated that CD43, one of the abundant surface glycoproteins, acts not only as potent co-receptor but also negative regulator for activation. Here we demonstrate co-stimulation human peripheral blood (PB) T cells through two distinct CD43 epitopes recognized by monoclonal antibodies (mAb) CD43-6E5 (T6E5-act ) and CD43-10G7 (T10G7-act potently induced proliferation....
The soluble cytoplasmic tail of CD45 (ct-CD45) is a cleavage fragment CD45, that generated during the activation human phagocytes. Upon release to extracellular space, ct-CD45 binds T cells and inhibits their in vitro. Here, we studied potential role TLR4 as receptor for ct-CD45. Treatment Jurkat TLR4/CD14 reporter with induced upregulation gene NFκB-eGFP could be blocked by inhibitors signaling. Conversely, did not promote NFκB-controlled eGFP induction expressing TLR1, TLR2, TLR6...
Abstract Co-receptors, being either co-stimulatory or co-inhibitory, play a pivotal role in T cell immunity, as they decide the fate of function. CD43 is one abundant surface glycoproteins expressed on cells. has been demonstrated to act not only potent co-receptor but also negative regulator for activation. To further investigate activation and subsequent function, peripheral blood cells were activated via two distinct epitopes recognized by monoclonal antibodies (mAbs) 6E5 (T6E5-act) 10G7...