A5039473029- Analytical chemistry methods development
- Probiotics and Fermented Foods
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Forensic Toxicology and Drug Analysis
- Analytical Chemistry and Chromatography
- Innovative Microfluidic and Catalytic Techniques Innovation
- Mass Spectrometry Techniques and Applications
- Animal Diversity and Health Studies
- Pharmacological Effects and Assays
- Electrochemical sensors and biosensors
- Analytical Chemistry and Sensors
- Nanoparticles: synthesis and applications
- Pesticide Residue Analysis and Safety
- Plant Pathogens and Fungal Diseases
- Microbial Inactivation Methods
- Metallurgy and Material Forming
- Diatoms and Algae Research
- Nanoplatforms for cancer theranostics
- Salmonella and Campylobacter epidemiology
- Food Industry and Aquatic Biology
- Advanced Nanomaterials in Catalysis
- Bacterial biofilms and quorum sensing
- Microfluidic and Capillary Electrophoresis Applications
University of Leicester
2018-2019
Fielding Graduate University
2018
Wrocław University of Science and Technology
2013-2016
IMT Mines Alès
2012
Commonwealth Scientific and Industrial Research Organisation
1956-1969
Dairy Australia (Australia)
1969
Health Sciences and Nutrition
1958
Epidermal growth factor receptor (EGFR), a tyrosine kinase receptor, is over-expressed in many tumors, including almost half of triple-negative breast cancers. The latter belong to very-aggressive and drug-resistant form malignancy. Although humanized anti-EGFR antibodies can work efficiently against these cancers both as monotherapy combination with genotoxic drugs, instability high production costs are some their known drawbacks clinical use. In addition, the development target membrane...
Rapid antigen tests are currently used for population screening of COVID-19. However, they lack sensitivity and utilize antibodies as receptors, which can only function in narrow temperature pH ranges. Consequently, molecularly imprinted polymer nanoparticles (nanoMIPs) synthetized with a fast (2 h) scalable process using merely tiny SARS-CoV-2 fragment (∼10 amino acids). The nanoMIPs rival the affinity under standard testing conditions surpass them at elevated temperatures or acidic media....
Molecularly Imprinted Polymers (MIPs) are synthetic receptors that able to selectively bind their target molecule and, for this reason, they currently employed as recognition elements in sensors. In work, MIP nanoparticles (nanoMIPs) produced by solid-phase synthesis a range of templates with different sizes, including small (biotin), two peptides (one derived from the epithelial growth factor receptor and vancomycin) protein (trypsin). NanoMIPs then dipcoated on surface thermocouples...
This manuscript describes the production of molecularly imprinted polymer nanoparticles (nanoMIPs) for cardiac biomarkers heart-fatty acid binding protein (H-FABP) and ST2 by solid-phase synthesis, their use as synthetic antibodies in a multiplexed sensing platform. Analysis surface plasmon resonance (SPR) shows that affinity nanoMIPs is similar to commercially available antibodies. The particles are coated onto thermocouples inserted into 3D-printed flow cells different designs. We...
Enzyme-linked immunosorbent assay (ELISA) is a widely used standard method for sensitive detection of analytes environmental, clinical, or biotechnological interest. However, ELISA has clear drawbacks related to the use relatively unstable antibodies and enzyme conjugates need several steps such as washing nonbound addition dye reagents. Herein, we introduce new completely abiotic where enzymes are replaced with fluorescent molecularly imprinted polymer nanoparticles (nanoMIPs)...
We demonstrate that a novel functionalized interface, where molecularly imprinted polymer nanoparticles (nanoMIPs) are attached to screen-printed graphite electrodes (SPEs), can be utilized for the thermal detection of cardiac biomarker troponin I (cTnI). The ultrasensitive unique protein cTnI early diagnosis myocardial infraction (i.e., heart attacks), resulting in considerably lower patient mortality and morbidity. Our developed platform presents an innovative route develop accurate,...
Summary In experimental small-scale manufacture of Cheddar cheese a study was made the movement lactose, lactic acid, calcium and phosphorus between curd whey, effect high acidity developed in before whey separation, on pH physical properties cheese. It found that while particles remain contact with lactose fermented is replaced by diffusing from whey. Lactic acid produced diffuses into rather slowly. When level attained rapidly does not cheddar well, low, flavour sour bitter, body crumbly...
We present here a novel screening tool for optimisation of polymerisation mixtures used in imprinting peptides and proteins. To facilitate rapid synthesis combinatorial library polymers the solid-phase method developed by Piletsky co-workers was scaled down to 50 mg template-immobilised solid phase, allowing single well 96-well microplate function as an individual reaction vessel. In this way, 32 different polymer compositions containing N-isopropylacrylamide, acrylic acid,...
A reaction closely resembling the nascent phage phenonomenon was studied. When lysing homologous strains of Str. lactis , two races were found to be capable simultaneously inducing lysis a heterologous strain and other phage-resistant strains. Seitz filtered potent lysates also caused lysis. Subjecting temperature 60°C. for 5 min. destroyed lytic activity on but did not inactivate phage. These produced haloes around their plaques while third phage, unable induce cultures, showed no haloes....
Sitagliptin is a hypoglycaemic agent used to reduce blood sugar levels in patients with type 2 diabetes mellitus (T2DM). Real time monitoring of sitagliptin crucial prevent overdose, which might cause liver, kidney and pancreatic diseases. As an alternative solution, voltammetric sensor was fabricated using artificial receptors called electroactive molecularly imprinted polymer nanoparticles (nanoMIPs). The nanoMIP tagged redox probe (ferrocene) combines both the recognition reporting...
Summary: Depression of activity certain single strain starter cultures in H.T.S.T. pasteurized milk during the October‐April season was observed, but effect removed by momentary boiling. The addition a small percentage such to autoclaved reduced these cultures. These ‘inhibited’cultures were unable grow modified Niven's medium even when additional known growth factors added. Using whey agar medium, active ‘uninhibited’colonies may be picked and ‘failing culture’revived. An hypothesis...
A novel bacterial quorum quenching system is presented. For the first time degradation of N-l-hexanoyl homoserine lactone (C6-AHL), a Gram-negative sensing autoinducer, has been enhanced using molecularly imprinted nanoparticles (MIP NPs) which were prepared transition state analogue γ-lactone ring hydrolysis as template.
This work presents the preparation and properties of molecularly imprinted polymers (MIPs) with catalytic centers that mimic active sites metalloenzymes. The MIP synthesis was based on suspension polymerization functional monomers (4-vinylpyridine acrylonitrile) trimethylolpropane trimethacrylate as a crosslinker in presence transition metal ions 4-methoxybenzyl alcohol template. Four have been chosen for imprinting from among microelements are most essential native enzymes: Cu 2+ , Co Mn Zn...
Abstract A novel abiotic assay based on biotin‐specific fluorescent molecularly imprinted polymer nanoparticles (nanoMIPs) which acted as both reporter probes and binding agents, was developed. This is a first report of an which, unlike ELISA, required no washing steps or addition enzyme substrates, making it more user‐friendly. The components the (MINA) were assembled in microtiter plates fitted with magnetic inserts. nanoMIPs bound to biotin‐conjugated particles, attracted free biotin...
A lysogenic culture, prepared in the laboratory from a strain of Streptococcus lactis , was used as cheese starter commercial factories. It attacked turn by two other unrelated phage races. The condition, which involved slight morphological and physiological changes, persisted subsequent forms resistant to one or both new Acquired resistance any three phages did not protect culture phages. In nature such interactions between races lactic acid bacteria must be constantly taking place, giving...
Here we introduce a new concept for synthesising molecularly imprinted nanoparticles by using proteins as macro-functional monomers. For proof-of-concept, model enzyme (HRP) was cross-linked glutaraldehyde in the presence of glass beads (solid-phase) bearing immobilized templates such vancomycin and ampicillin. The cross-linking process links together protein chains, which leads to formation permanent target-specific recognition sites without adverse effects on enzymatic activity. Unlike...
The problem of bacteriophage in cheesemaking has been reviewed recently by Whitehead(1) and Mattick(2). Results since reported other workers ourselves call for some modification addition to the existing concepts problem.
Alginate was used for the immobilization of an alkylphosphonium ionic liquid (IL). The IL-alginate resin served Pd(II) binding from HCl solutions. After chemical reduction Pd(II), composite tested hydrogenation nitrophenols (NPs) and 4-nitroaniline (4-NA) using sodium formate or formic acid as hydrogen donor (HD) in batch system. influence agitation speed, particle size, type, concentration HD pH has been evaluated 2-NP, 3-NP, 4-NP, 4-NA (into 2-aminophenol, 2-AP, 3-AP, 4AP...