A5062085346- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Force Microscopy Techniques and Applications
- Cell Adhesion Molecules Research
- Skin and Cellular Biology Research
- Tribology and Lubrication Engineering
- Surface Treatment and Residual Stress
- Advanced Electron Microscopy Techniques and Applications
- Adhesion, Friction, and Surface Interactions
University of California, Davis
2019-2022
Iowa State University
2018-2021
Significance Cadherin cell–cell adhesion proteins play key roles in the formation and maintenance of tissues. Their is carefully regulated to orchestrate complex movement cells. While cadherin ectodomains bind two conformations with different adhesive properties, mechanisms by which cells regulate conformation (and consequently adhesion) individual cadherins are unknown. Here, we demonstrate that association intracellular vinculin cytoplasmic region regulates switching from a weak binding...
ABSTRACT Cadherin cell-cell adhesion proteins play key roles in tissue morphogenesis and wound healing. ectodomains bind two conformations, X-dimers strand-swap dimers, with different adhesive properties. However, the mechanisms by which cells regulate ectodomain conformation are unknown. intracellular regions associate several actin-binding including vinculin, believed to tune remodeling actin cytoskeleton. Here, we show at single molecule level, that vinculin association cadherin...
Cadherins are essential cell-cell adhesion proteins that interact in two distinct conformations: X-dimers and strand-swap dimers. Both dimers thought to exclusively rely on symmetric sets of interactions between key amino acids both cadherin binding partners. Here, we use single-molecule atomic force microscopy computer simulations show symmetry is dispensable cadherins can also a novel conformation asymmetrically incorporates elements X-dimers. Our results clarify the biophysical rules for...