Abstract Plant and fungal laccases belong to the family of multi‐copper oxidases show much broader substrate specificity than other members family. Laccases have consequently been interest for potential industrial applications. We analyzed essential sequence features based on multiple alignments more 100 laccases. This has resulted in identification a set four ungapped regions, L1–L4, as overall signature sequences that can be used identify laccases, distinguishing them within class...

Horseradish peroxidase (HRP) is known to degrade certain recalcitrant organic compounds such as phenol and substituted phenols. Here, for the first time we have shown HRP be effective in degrading precipitating industrially important azo dyes. For Remazol blue, enzyme activity was found far better at pH 2.5 than neutral pH. In addition, blue acts a strong competitive inhibitor of shows broad substrate specificity toward variety Kinetic constants (K(m)(app) V(max)(app)) two different dyes...

Abstract Cyanobacteria provide an interesting platform for biotechnological applications due to their efficient photoautotrophic growth, amenability genetic engineering and the ability grow on non-arable land. An ideal industrial strain of cyanobacteria would need be fast growing tolerant high levels temperature, light, carbon dioxide, salt naturally transformable. In this study, we report Synechococcus elongatus PCC 11801, a isolated from India that fulfills these requirements. The...

Lipases are versatile catalysts. In addition to their natural reaction of fat hydrolysis, lipases catalyze a plethora other reactions such as esterification, amidation, and transesterification esters well organic carbonates. Moreover, accept wide variety substrates while maintaining regioselectivity stereoselectivity. highly stable even under adverse conditions solvents, high temperatures, so forth. Applications include production food additives, chiral intermediates, pharmaceutical...

Cyanobacteria, which constitute a quantitatively dominant phylum, have attracted attention in biofuel applications due to favorable physiological characteristics, high photosynthetic efficiency and amenability genetic manipulations. However, quantitative aspects of cyanobacterial metabolism received limited attention. In the present study, we performed isotopically non-stationary 13 C metabolic flux analysis (INST-13 C-MFA) analyze rerouting carbon glycogen synthase deficient mutant strain...

Cyanobacteria, a group of photosynthetic prokaryotes, are attractive hosts for biotechnological applications. It is envisaged that future biorefineries will deploy engineered cyanobacteria the conversion carbon dioxide to useful chemicals via light-driven, endergonic reactions. Fast-growing, genetically amenable, and stress-tolerant desirable as chassis such The recently reported strains Synechococcus elongatus UTEX 2973 PCC 11801 hold promise, but additional may be needed ongoing efforts...

Enzyme structure and function have been studied for subtilisin BPN' solubilized in organic solvents by ion pairing with low concentrations of an anionic surfactant (Aerosol OT) the absence reversed micelles. Soluble shows strikingly different behavior octane tetrahydrofuran (THF). In octane, kcat/Km transesterification N-acetyl-l-phenylalanine ethyl ester (APEE) is 370 M-1 s-1, within one order magnitude enzyme's hydrolytic activity water. Moreover, observed half-life soluble enzyme nearly...

Abstract Motivation: Dynamic Bayesian networks (DBN) are widely applied in modeling various biological including the gene regulatory network (GRN). Due to NP-hard nature of learning static structure, most methods for DBN also employ either local search such as hill climbing, or a meta stochastic global optimization framework genetic algorithm simulated annealing. Results: This article presents GlobalMIT, toolbox globally optimal structure from expression data. We propose using recently...

Abstract Background Dynamic Bayesian network (DBN) is among the mainstream approaches for modeling various biological networks, including gene regulatory (GRN). Most current methods learning DBN employ either local search such as hill-climbing, or a meta stochastic global optimization framework genetic algorithm simulated annealing, which are only able to locate sub-optimal solutions. Further, applications have essentially been limited small sized networks. Results To overcome above...

Abstract Background and Aim Tuberculosis ( TB ) is a major public health problem in I ndia. Despite the treatment availability monitoring, drug‐induced hepatotoxicity DIH serious concern can lead to discontinuation of treatment. Anti‐ well known aggravate because pharmacokinetic pharmacodynamic interactions. Genetic polymorphism drug‐metabolizing enzyme genes an important factor that predisposes certain fraction population toxicity. The purpose this study was assess association N...

Cyanobacteria, a group of photosynthetic prokaryotes, are being increasingly explored for direct conversion carbon dioxide to useful chemicals. However, efforts engineer these photoautotrophs have resulted in low product titers. This may be ascribed the bottlenecks metabolic pathways, which need identified rational engineering. We engineered recently reported, fast-growing and robust cyanobacterium, Synechococcus elongatus PCC 11801 produce succinate, an important platform chemical....

Cyanobacteria are emerging as hosts for photoautotrophic production of chemicals. Recent studies have attempted to stretch the limits photosynthetic production, typically focusing on one product at a time, possibly minimise additional burden separation. Here, we explore simultaneous two products that can be easily separated: ethylene, gaseous product, and succinate, an organic acid accumulates in culture medium. This was achieved by expressing single copy ethylene forming enzyme (efe) under...

Abstract Dynamic flux balance analysis (FBA) allows estimation of intracellular reaction rates using organism‐specific genome‐scale metabolic models (GSMM) and by assuming instantaneous pseudo‐steady states for processes that are inherently dynamic. This technique is well‐suited industrial bioprocesses employing complex media characterized a hierarchy substrate uptake product secretion. However, knowledge exchange many components the would be required to obtain meaningful results. Here, we...

Synechococcus elongatus PCC 11801 is a fast-growing cyanobacterium, exhibiting high tolerance to environmental stresses. We have earlier characterized its genome and analysed transcriptome proteome. However, deploy it as potential cell factory, necessary expand synthetic biology toolbox, including promoter elements ribosome binding sites (RBSs). Here, based on the global analysis, 48 native promoters of genes with transcript count were using fluorescent reporter system. The P