Mohamed A. Abouelkhair

ORCID: 0000-0003-0109-6000
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About
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Research Areas
  • Antimicrobial Resistance in Staphylococcus
  • SARS-CoV-2 and COVID-19 Research
  • Bacterial Identification and Susceptibility Testing
  • Respiratory viral infections research
  • Animal Virus Infections Studies
  • COVID-19 Clinical Research Studies
  • Bacterial biofilms and quorum sensing
  • Bacteriophages and microbial interactions
  • Microbial Metabolism and Applications
  • Infective Endocarditis Diagnosis and Management
  • Biochemical and Structural Characterization
  • Streptococcal Infections and Treatments
  • Pharmaceutical and Antibiotic Environmental Impacts
  • Viral Infections and Vectors
  • Orthopedic Infections and Treatments
  • Adenosine and Purinergic Signaling
  • Microbial infections and disease research
  • COVID-19 Impact on Reproduction
  • Virus-based gene therapy research
  • Genomics and Phylogenetic Studies
  • Plant Virus Research Studies
  • Antimicrobial Peptides and Activities
  • Bee Products Chemical Analysis
  • Viral Infections and Outbreaks Research
  • Antibiotic Use and Resistance

University of Tennessee at Knoxville
2018-2025

Knoxville College
2018-2025

Rowan University
2025

University of Sadat City
2017-2020

Foods that contain antibiotic residues have potential adverse health effects on consumers and provide selective pressure for the threat of antimicrobial resistance (AMR). This study's objective was to measure tetracycline, sulfonamide, erythromycin in beef, eggs, honey sold as "antibiotic-free" at farmers' markets East Tennessee (East TN) United States (U.S.). Between July September 2020, 36 food products (9 18 egg, 9 products) were purchased from TN tested using competitive enzyme-linked...

10.3390/vetsci10040243 article EN cc-by Veterinary Sciences 2023-03-24

Coyotes are exposed to many parasites and pathogens of veterinary zoonotic concern. To assess the prevalence diseases caused by these microbes, we opportunistically obtained coyote samples from a variety sources including GPS collaring study, rabies testing facilities, wildlife resources agents, road-side mortalities. We performed necropsies, serological testing, fecal flotations, molecular analyses on coyotes Tennessee South Carolina. Dirofilaria immitis (heartworm) infected 46% (41/89) was...

10.1371/journal.pone.0318645 article EN cc-by PLoS ONE 2025-02-10

ABSTRACT We report the genome sequence of a strain belonging to species Tetraparvovirus ungulate 1 (hereafter referred as UTPV1-1882), identified in 4-month-old cow with respiratory disease Tennessee, USA. The data will improve existing genomic database, which currently lacks information on these viruses United States.

10.1128/mra.01291-24 article EN Microbiology Resource Announcements 2025-03-25

ABSTRACT We report the genome sequence of a bovine respiratory syncytial virus strain (hereafter referred to as BRSV_23), identified in cow Tennessee. This updates currently circulating BRSV field strains United States, providing insights into viral evolution and epidemiology improve diagnostics control strategies.

10.1128/mra.00072-25 article EN Microbiology Resource Announcements 2025-04-28

Bacterial infections from Staphylococcus pseudintermedius are the most common cause of skin (pyoderma) affecting dogs. Two component pore-forming leukocidins a family potent toxins secreted by staphylococci and consist S (slow) F (fast) components. They impair innate immune system, first line defense against these pathogens. Seven different have been characterized in aureus, some which host cell specific. Through genome sequencing analysis S. secretome using liquid chromatography mass...

10.1371/journal.pone.0204450 article EN cc-by PLoS ONE 2018-09-27

Background: Staphylococcus pseudintermedius is an opportunistic pathogen that the major cause of pyoderma affecting dogs. Conventional antimicrobial treatment for infections caused by this organism have failed in recent years due to widespread resistance and alternative strategies are a high priority. Protein A encoded aureus spa protects bacterium binding IgG acts as superantigen. possess two genes orthologous S. spa, spsP, spsQ. Methods: SpsQ SpsQ-M, non-toxigenic SpsQ, were cloned...

10.1080/21505594.2018.1489199 article EN cc-by-nc Virulence 2018-07-27

Background In December 2019, an ongoing outbreak of pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2/ 2019-nCoV) infection was initially reported in Wuhan, Hubei Province, China. Early 2020, the World Health Organization (WHO) announced a new name for 2019-nCoV-caused disease: disease 2019 (COVID-19) and declared COVID-19 to be Public Emergency International Concern (PHEIC). Cellular co-infection is critical determinant viral fitness outcomes plays crucial role...

10.7717/peerj.10246 article EN cc-by PeerJ 2020-11-02

Staphylococcus pseudintermedius is an important cause of clinical infections in small-animal-veterinary medicine. Evolutionary changes strains using multilocus sequence typing (MLST) have been observed among S . European countries and the United States. However, there are limited or no studies on detection methicillin resistant (MRSP) predominating MLST South Africa. Therefore, this study aimed to determine molecular epidemiology Twenty-six, non-duplicate, isolates from dogs were obtained as...

10.1371/journal.pone.0290645 article EN cc-by PLoS ONE 2023-08-30

We report here the first whole-genome sequences for 3 strains of Staphylococcus pseudintermedius (112N, 113N, and 114N) isolated in Africa. Samples this opportunistic pathogen were collected from nasal swabs obtained healthy carrier dogs Botswana. The sequence information will facilitate spatial phylogenetic comparisons staphylococcal species other bacteria at genome level.

10.1128/genomea.01599-17 article EN Genome Announcements 2018-03-07

Staphylococci have evolved numerous strategies to evade their hosts’ immune systems. Some staphylococcal toxins target essential components of host innate immunity, one the two main branches system. Analysis Staphylococcus pseudintermedius secretome using liquid chromatography mass spectrometry guided by genomic data, was used identify an S. exotoxin provisionally named SpEX. This exoprotein has low overall amino acid identity with aureus group proteins superantigen like (SSLs) and...

10.1371/journal.pone.0220301 article EN public-domain PLoS ONE 2019-07-29

ABSTRACT We report the first complete genome sequence of LMG 22219 (=ON 86 T = CCUG 49543 ), Staphylococcus pseudintermedius type strain isolated from feline lung tissue. This information will facilitate phylogenetic comparisons staphylococcal species and other bacteria at level.

10.1128/genomea.01651-16 article EN Genome Announcements 2017-02-16

Here, we announce the complete coding sequence of two strains feline panleukopenia virus (FPLV) that were obtained from deceased domestic cats in animal shelters Tennessee. The provided data will contribute to a deeper comprehension genetic characteristics and evolutionary patterns FPLV USA.

10.1128/mra.00431-23 article EN Microbiology Resource Announcements 2023-09-28

In December 2019, pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection emerged in Wuhan City, Hubei Province, China. Early 2020, the World Health Organization (WHO) announced a new name for 2019-nCoV-caused epidemic disease: disease 2019 (COVID-19) and declared COVID-19 to be sixth international public health emergency. Cellular co-infection is critical determinant of both viral fitness outcome plays crucial role shaping host immune response infections....

10.20944/preprints202005.0505.v2 preprint EN 2020-06-18

We report here the genome sequences of 11 canine Staphylococcus pseudintermedius isolates from New York, Hampshire, California, Pennsylvania, and Kansas. The sequencing information will enable spatial phylogenetic comparisons staphylococcal species other related help in better understanding their virulence potential.

10.1128/mra.00002-23 article EN Microbiology Resource Announcements 2023-03-06

This study evaluated the concentrations of tetracycline, erythromycin, and sulfonamide in beef, eggs, honey products sold at grocery stores whether standard cooking to 160 °F (71 °C) reduced antimicrobials spiked into food products. In June 2021, selected 65 samples (8 48 9 samples) purchased from within Knoxville, Tennessee, were measured using enzyme-linked immunosorbent assays. The Kruskal–Wallis test was used assess differences median tetracycline across types, while Mann–Whitney compare...

10.3390/vetsci11120660 article EN cc-by Veterinary Sciences 2024-12-17

Staphylococcus aureus is the causative agent of multiple infections, including bacteremia, infective endocarditis, osteomyelitis, septic arthritis, and prosthetic device infections. We report here first whole-genome sequence for four S. type 398 isolates from clinical cases osteomyelitis in goats with a history orthopedic surgery.

10.1128/mra.01174-18 article EN Microbiology Resource Announcements 2018-09-26

Staphylococcus aureus (SA) is a significant and well-recognized causative organism of bacterial osteomyelitis. Osteomyelitis an inflammatory bone disease characterized by progressive destruction loss. This causes morbidity mortality to the patient poses therapeutic challenges for clinicians. To improve efficacy strategies combat osteomyelitis, there need define molecular epidemiology organisms more clearly further understanding pathogenesis SA We conducted in vitro characterization...

10.3389/fcimb.2022.1015655 article EN cc-by Frontiers in Cellular and Infection Microbiology 2022-11-24

In December 2019, pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection emerged in Wuhan City, Hubei Province, China. Early 2020, the World Health Organization (WHO) announced a new name for 2019-nCoV-caused epidemic disease: disease 2019 (COVID-19) and declared COVID-19 to be sixth international public health emergency. Cellular co-infection is critical determinant of both viral fitness outcome plays crucial role shaping host immune response infections....

10.20944/preprints202005.0505.v1 preprint EN 2020-05-31

The most serious health issue today is the rapid outbreak of Coronavirus Disease 2019 (COVID-19 or SARS-CoV-2). It now a global pandemic and huge concern for public health. So far, more than 3,500,000 confirmed cases were diagnosed in nearly 212 countries territories around world 2 international conveyances, causing globally over 250,000 deaths. Epidemiology, risk factors, clinical characteristics COVID-19 patients have been identified but factors influencing immune system against not well...

10.31219/osf.io/9b2hu preprint EN 2020-05-10
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