A5049881877- Genetics, Aging, and Longevity in Model Organisms
- Insect symbiosis and bacterial influences
- Parasitic Infections and Diagnostics
- Nematode management and characterization studies
- CRISPR and Genetic Engineering
- Evolution and Genetic Dynamics
- Gut microbiota and health
- Parasite Biology and Host Interactions
- Pluripotent Stem Cells Research
- Vibrio bacteria research studies
- Renal and related cancers
- Protist diversity and phylogeny
- Parasites and Host Interactions
- HIV-related health complications and treatments
- Plant Disease Resistance and Genetics
- Birth, Development, and Health
- Kruppel-like factors research
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Escherichia coli research studies
- Reproductive Health and Technologies
- Malaria Research and Control
University of Toronto
2018-2025
New York University
2020
Parental infection can result in the production of offspring with enhanced immunity phenotypes. Critically, mechanisms underlying inherited are poorly understood. Here, we show that Caenorhabditis elegans infected intracellular microsporidian parasite N. parisii produce progeny resistant to microsporidia infection. We determine kinetics response and intergenerational prevents host-cell invasion by Nematocida enhances survival bacterial pathogen Pseudomonas aeruginosa demonstrate is induced...
Cooperative action of a transcription factor complex containing OCT4, SOX2, NANOG, and KLF4 maintains the naive pluripotent state; however, less is known about mechanisms that disrupt this complex, initiating exit from pluripotency. We show that, as embryonic stem cells (ESCs) pluripotency, protein exported nucleus causing rapid decline in Nanog Klf4 transcription; result, first pluripotency removed transcription-associated complexes during differentiation. nuclear export requires ERK...
Microsporidia are a large phylum of intracellular parasites that can infect most types animals. Species in the Nematocida genus nematodes including Caenorhabditis elegans, which has become an important model to study mechanisms microsporidia infection. To understand genomic properties and evolution nematode-infecting microsporidia, we sequenced genomes nine species two genera, Enteropsectra Pancytospora, without any previously genomes. Core cellular processes, metabolic pathways, mostly...
Microsporidia are ubiquitous obligate intracellular pathogens of animals. These parasites often infect hosts through an oral route, but little is known about the function host intestinal proteins that facilitate microsporidia invasion. To identify such factors necessary for infection by
Microsporidia are fungal-related obligate intracellular parasites that infect many types of animals. have exceptionally reduced genomes resulting in limited metabolic capabilities and thought to be reliant on host metabolism fuel their own growth. Here, we investigate the impact microsporidia infection lipid using nematode Caenorhabditis elegans along with its natural microsporidian pathogen Nematocida parisii. We show causes an increase level C. droplet associated lipase, ATGL-1, a decrease...
Host diversity can affect parasite prevalence, a phenomenon widely studied in macroscopic organisms. However, data from microscopic communities are lacking, despite their essential role ecosystem functioning and the unique experimental opportunities organisms offer. Here, we study diversity–disease effects wild nematode by profiting molecular tools available well-studied model Caenorhabditis elegans . Nanopore sequencing was used to characterize community composition, whereas parasites were...
Abstract The mechanisms behind the ability of Plasmodium falciparum to evade host immune system are poorly understood and a major roadblock in achieving malaria elimination. Here, we use integrative genomic profiling longitudinal pediatric cohort Burkina Faso demonstrate role post-transcriptional regulation response malaria. We report strong signature miRNA expression differentiation associated with P. infection (127 out 320 miRNAs, B-H FDR 5%) parasitemia (72 5%). Integrative miRNA-mRNA...
Inherited immunity describes how some animals can pass on the "memory" of a previous infection to their offspring. This boost pathogen resistance in progeny and promote survival. While inherited has been reported many invertebrates, mechanisms underlying this epigenetic phenomenon are largely unknown. The Caenorhabditis elegans by natural microsporidian Nematocida parisii results worms producing offspring that robustly resistant microsporidia. present protocol study intergenerational simple...
Nematodes are naturally infected by the fungal-related pathogen microsporidia. These ubiquitous eukaryotic parasites poorly understood, despite infecting most types of animals. Identifying novel species microsporidia and studying them in an animal model can expedite our understanding their infection biology evolution. present excellent avenue for pursuing such work, as they abundant environment many easily culturable laboratory. The protocols presented here describe how to isolate...
Abstract Microsporidia are eukaryotic obligate intracellular parasites that infect most animals including humans. To understand how the microbiome can impact microsporidia infection, we tested bacterial isolates naturally occur with Caenorhabditis elegans influence infection by microsporidian Nematocida parisii . Nematodes exposed to two of these bacteria, Chryseobacterium scopthalmum and Sphingobacterium multivorum , exhibit reduced pathogen loads. Using untargeted metabolomics, show...
Abstract Microsporidia are a large phylum of intracellular parasites that can infect most types animals. Species in the Nematocida genus nematodes including Caenorhabditis elegans , which has become an important model to study mechanisms microsporidia infection. To understand genomic properties and evolution nematode-infecting microsporidia, we sequenced genomes nine species two genera, Enteropsectra Pancytospora, without any previously genomes. Core cellular processes, metabolic pathways,...
Abstract Inherited immunity is an emerging field and describes how the transfer of from parents to offspring can promote progeny survival in face infection. The mechanisms inherited induced are mostly unknown. intracellular parasite Nematocida parisii a natural microsporidian pathogen Caenorhabditis elegan s. Here, we show that N. -infected worms produce primed resistant microsporidia We find dose dependent manner lasts for single generation. Intergenerational prevents host cell invasion by...
Abstract Microsporidia are ubiquitous obligate intracellular pathogens of animals. These parasites often infect hosts through an oral route, but little is known about the function host intestinal proteins that facilitate microsporidia invasion. To identify such factors necessary for infection by Nematocida parisii , a natural microsporidian pathogen Caenorhabditis elegans we performed forward genetic screen to mutant animals have Fitness Advantage with (Fawn). We isolated four fawn mutants...
Abstract Biodiversity can affect parasite prevalence, a phenomenon widely studied in macroscopic organisms. However, data from microscopic communities is lacking, despite their essential role ecosystem functioning and the unique experimental opportunities organisms offer. Here, we study diversity-disease effects wild nematode by profiting molecular tools available well-studied model Caenorhabditis elegans . Nanopore sequencing was used to characterize community diversity composition, whereas...
Inherited immunity describes how some animals can pass on the "memory" of a previous infection to their offspring. This boost pathogen resistance in progeny and promote survival. While inherited has been reported many invertebrates, mechanisms underlying this epigenetic phenomenon are largely unknown. The Caenorhabditis elegans by natural microsporidian Nematocida parisii results worms producing offspring that robustly resistant microsporidia. present protocol study intergenerational simple...