A5038954346- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Cancer Diagnosis and Treatment
- Metastasis and carcinoma case studies
- Cancer Research and Treatments
- Medical Imaging and Pathology Studies
- Extracellular vesicles in disease
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Fibroblast Growth Factor Research
- Lung Cancer Research Studies
- Ferroptosis and cancer prognosis
- Histiocytic Disorders and Treatments
- Esophageal Cancer Research and Treatment
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Lung Cancer Treatments and Mutations
- Immune cells in cancer
- Microfluidic and Bio-sensing Technologies
- Cancer Mechanisms and Therapy
- Sarcoma Diagnosis and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced Breast Cancer Therapies
- Immune Response and Inflammation
- Eosinophilic Disorders and Syndromes
University of Bologna
2015-2025
Azienda USL di Bologna
2025
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2014-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2022
Creative Commons
2020
Novartis (Germany)
2020
Roche (Switzerland)
2020
Weatherford College
2020
University of Ferrara
2013
Abstract Epithelial mesenchymal transition (EMT) is a physiological process necessary to normal embryologic development. However in genesis of pathological situations, this can be perverted and signaling pathways have different regulations from those physiology. In cancer invasion, such mechanism leads generation circulating tumor cells. cells become motile able shed the primary enter blood circulation. This major part invasive way cancer. EMT also implicated chronic diseases like fibrosis...
Abstract Tumor dissemination is a key event in tumor progression. During this event, main role played by circulating cells (CTCs), immune cells, and their interaction. How the system supports survival proliferation of CTCs not fully elucidated. In study we established an in-vitro co-culture consisting from same patient, which increased success rate establishment CTC-derived long-term cell cultures. system, characterized successful co-cultures signals they exchange with cancer including...
Circulating tumor cells (CTCs) are a rare population of representing key player in the metastatic cascade. They recognized as validated tool for identification patients with higher risk relapse, including those diagnosed breast cancer (BC). However, CTCs characterized by high levels heterogeneity that also involve copy number alterations (CNAs), structural variations associated gene dosage changes. In this study, single were isolated from peripheral blood 11 early-stage BC at different time...
Epithelial-to-mesenchymal transition (EMT) has been shown to be associated with tumor progression and metastasis. During this process in breast cancer, a crucial role is played by alternative splicing systems. To identify new early prognostic marker of metastasis, we evaluated EMT-related gene expression cell lines, primary tissue from 31 patients focusing our attention on factors ESRP1, ESRP2 RBFOX2. Results showed that the patterns these genes were indicative onset EMT in-vitro models, but...
<title>Abstract</title> <bold>Background</bold> The extracellular vesicle (EV) pathway plays a critical role in cell-to-cell communication. Cancer cells release EVs into the space, where they interact with both cancerous and noncancerous cells, activating signaling pathways remodeling tumor microenvironment (TME). In this study, we investigated functional of released by FGFR2-amplified cancers unknown primary (CUPs), which generate extrachromosomal circular DNA (ecDNA) to enhance oncogenic...
Abstract The extracellular vesicle (EV) route is essential for cell-to-cell communication. Cancer cells release EVs in the space, where they can interact with cancer and non-cancer cells, activating specific signaling pathways, modulating tumor microenvironment remodeling inducing gene expression alterations. We investigated functional role of released by unknown primary (CUP), a rare disease (1-3% novel diagnoses) that presents metastasis or uncertain origin no apparent tumor. derived three...
Patients with cancer of unknown primary (CUP) carry the double burden an aggressive disease and reduced access to therapies. Experimental models are pivotal for CUP biology investigation drug testing. We derived two cell lines (CUP#55 #96) corresponding patient-derived xenografts (PDXs), from ascites tumor cells. PDXs underwent histological, immune-phenotypical, molecular, genomic characterization confirming features original tumor. The tissue-of-origin prediction was obtained microRNA...
BackgroundDespite their lethality and ensuing clinical therapeutic relevance, circulating tumor cells (CTCs) from colorectal carcinoma (CRC) remain elusive, poorly characterized biological entities.Methods findingsWe perfected a cell system of stable, primary lines human CRC showing that they possess the full complement ex- in-vivo, in xenogeneic models, characteristics stem (CCSCs). Here we show how tumor-initiating, CCSCs can establish faithful orthotopic phenocopies original disease,...
Circulating tumor cells' (CTCs) heterogeneity contributes to counteract their introduction in clinical practice. Through single-cell sequencing we aim at exploring CTC metastatic breast cancer (MBC) patients. Single CTCs were isolated using DEPArray NxT. After whole genome amplification, libraries prepared for copy number aberration (CNA) and single nucleotide variant (SNV) analysis sequenced Ion GeneStudio S5 Illumina MiSeq, respectively. demonstrate distinctive mutational signatures but...
Breast cancer (BC) is the most commonly diagnosed malignant tumor in women worldwide, and leading cause of death female population. The percentage patients experiencing poor prognosis along with risk developing metastasis remains high, also affecting resistance to current main therapies. Cancer progression metastatic development are no longer due entirely their intrinsic characteristics, but regulated by signals derived from cells microenvironment. Extracellular vesicles (EVs) packed DNA,...
OPINION article Front. Oncol., 11 January 2017Sec. Molecular and Cellular Oncology Volume 6 - 2016 | https://doi.org/10.3389/fonc.2016.00275
Circulating tumor cells (CTCs) are associated with poor survival in metastatic cancer. Their identification, phenotyping, and genotyping could lead to a better understanding of heterogeneity thus facilitate the selection patients for personalized treatment. However, this is hampered because rarity CTCs. We present an innovative approach sampling high volume patient blood obtaining information about presence, phenotype, gene translocation The method combines immunofluorescence staining DNA...
Combining phenotypical and molecular characterization of rare cells is challenging due to their scarcity difficult handling. In oncology, circulating tumor (CTCs) are considered among the most important cell populations. Their phenotypic necessary define mechanisms underlying metastatic potential. Several approaches that require fixation make downstream investigations on RNA. Conversely, DEPArray technology allows analysis handling both fixed unfixed cells, enabling a wider range...
Here, we monitored the evolution of CTCs spread in 11 patients affected by locally advanced EC who were undergoing therapy.In this perspective study, designed multiple blood biopsies from individual patients: before and after neoadjuvant chemo-radio therapy surgery. We developed a multi-target array, named Grab-all assay, to estimate for their epithelial (EpCAM/E-Cadherin/Cytokeratins) mesenchymal/stem (N-Cadherin/CD44v6/ABCG2) phenotypes. Identified isolated as single cells DEPArray,...