A5067392968- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoreceptor and optogenetics research
- Ion Channels and Receptors
- Alzheimer's disease research and treatments
- Ion channel regulation and function
- Neurogenesis and neuroplasticity mechanisms
- Nuclear Receptors and Signaling
- Cell Image Analysis Techniques
- Memory and Neural Mechanisms
- Biochemical Analysis and Sensing Techniques
- Medical Imaging Techniques and Applications
Goethe University Frankfurt
2020-2021
University College London
2018
GlaxoSmithKline (United Kingdom)
2018
Duke-NUS Medical School
2015-2016
Recent findings point to a central role of the endoplasmic reticulum-resident STIM (Stromal Interaction Molecule) proteins in shaping structure and function excitatory synapses mammalian brain. The impact Stim genes on cognitive functions remains, however, poorly understood. To explore learning memory, we generated three mouse strains with conditional deletion (cKO) Stim1 and/or Stim2 forebrain. Stim1, Stim2, double Stim1/Stim2 cKO mice show no obvious brain structural defects or locomotor...
STIMs (STIM1 and STIM2 in mammals) are transmembrane proteins that reside the endoplasmic reticulum (ER) regulate store-operated Ca 2+ entry (SOCE). The function of brain is only beginning to be explored, relevance SOCE nerve cells being debated. Here we identify as a central organizer excitatory synapses. STIM2, but not its paralogue STIM1, influences formation dendritic spines shapes basal synaptic transmission neurons. We further demonstrate essential for cAMP/PKA-dependent...
Large spines are stable and important for memory trace formation. The majority of large also contains synaptopodin (SP), an actin-modulating plasticity-related protein. Since SP stabilizes F-actin, we speculated that the presence within could explain their long lifetime. Indeed, using 2-photon time-lapse imaging SP-transgenic granule cells in mouse organotypic tissue cultures found containing survived considerably longer than equal size without SP. Of note, SP-positive (SP+) underwent...
The entorhino-dentate projection, i.e., the perforant pathway, terminates in a highly ordered and laminated fashion rodent dentate gyrus (DG): fibers arising from medial entorhinal cortex (MEC) terminate middle molecular layer, whereas lateral (LEC) outer layer of DG. In rats rabbits, crossed projection exists, which originates (EC) contralateral contrast, mice, such is reportedly absent. Using single double mouse organotypic entorhino-hippocampal slice cultures, we studied ipsi-...
Abstract Background Late stage Alzheimer’s disease and other dementias are associated with neurofibrillary tangles neurodegeneration. Here we describe a mouse (TauD35) carrying human Tau the P301L mutation that results in hyperphosphorylation tangles. Previously have compared gene expression TauD35 mice to which develop plaques but no A similar comparison of pathological features throughout progression is made here between amyloidβ described Parts I II this study. Methods In vitro CA1 patch...
Abstract Large spines are stable and important for memory trace formation. The majority of large also contains Synaptopodin (SP), an actin-modulating plasticity-related protein. Since SP stabilizes F-actin, we speculated that the presence within could explain their long lifetime. Indeed, using time-lapse 2-photon-imaging SP-transgenic granule cells in mouse organotypic tissue cultures found containing survived considerably longer than equal size without SP. Of note, SP-positive underwent...
Abstract Background Progression of Alzheimer’s disease is thought initially to depend on rising amyloidβ and its synaptic interactions. Transgenic mice (TASTPM; APP Swe / PSEN1 M146V ) show altered transmission, compatible with increased physiological function amyloidβ, before plaques are detected. Recently, the importance microglia has become apparent in human disease. Similarly, TASTPM a close association plaque load upregulated microglial genes. Methods CA1 Synaptic transmission...