A5028810073- Glaucoma and retinal disorders
- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Microbial metabolism and enzyme function
- Retinal Diseases and Treatments
- Alcohol Consumption and Health Effects
- Cell Adhesion Molecules Research
- Ocular Surface and Contact Lens
- Atherosclerosis and Cardiovascular Diseases
- Connective tissue disorders research
- Long-Term Effects of COVID-19
- Dermatological and Skeletal Disorders
- Proteoglycans and glycosaminoglycans research
- Connexins and lens biology
- Clusterin in disease pathology
- Protease and Inhibitor Mechanisms
- Ophthalmology and Eye Disorders
- Retinal Development and Disorders
- Peroxisome Proliferator-Activated Receptors
- Developmental Biology and Gene Regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ubiquitin and proteasome pathways
- Marine Ecology and Invasive Species
- Glycosylation and Glycoproteins Research
- COVID-19 Clinical Research Studies
Friedrich-Alexander-Universität Erlangen-Nürnberg
2015-2025
Universitätsklinikum Erlangen
2011-2025
Ophthalmology Associates (United States)
2010-2011
The aim of this study was to investigate the transdifferentiation potential murine vibrissa hair follicle (HF) stem cells into corneal epithelial-like through modulation by corneal- or limbus-specific microenvironmental factors. Adult epithelial were isolated from HF bulge region mechanical dissection fluorescence-activated cell sorting using antibodies alpha6 integrin, enriched clonal expansion, and subcultivated on various extracellular matrices (type IV collagen, laminin-1, laminin-5,...
Long COVID (LC) describes the clinical phenotype of symptoms after infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnostic and therapeutic options are limited, as pathomechanism LC is elusive. As number SARS-CoV-2 infections was large, will be a challenge for healthcare system. Previous studies revealed an impaired blood flow, formation microclots, autoimmune mechanisms potential factors in this complex interplay. Since functionally active autoantibodies...
To identify and characterize genes differentially expressed in anterior segment tissues of eyes with pseudoexfoliation (PEX) syndrome glaucoma.Anterior (iris, ciliary processes, lens epithelium) were obtained from eight surgically enucleated PEX-associated open-angle or closed-angle glaucomas age-matched glaucomatous control without PEX. cDNA libraries generated three PEX specimens, their gene expression patterns compared by means subtraction. Differentially clones the subtracted sequenced,...
Purpose.: Pseudoexfoliation (PEX) syndrome/glaucoma is a complex, late-onset disorder of the elastic fiber system. Strong genetic risk conferred by lysyl oxidase-like 1 (LOXL1) gene, but additional comodulating factors are necessary for manifestation disease. The aim this study was to analyze effect various PEX-associated pathogenic on genotype-correlated expression LOXL1 and elastin-related genes. Methods.: Cultured human Tenon's capsule fibroblasts with high- low-risk haplotypes were...
Interactions between stem cells and their microenvironment are critical for regulation maintenance of cell function. To elucidate the molecular interactions within human limbal epithelial stem/progenitor (LEPC) niche, which is essential maintaining corneal transparency vision, we performed a comprehensive expression analysis adhesion molecules (CAMs) using custom-made quantitative real-time polymerase chain reaction (qRT-PCR) arrays laser capture-microdissected LEPC clusters, comprising...
To characterize the alterations of extracellular matrix proteins in Descemet's membranes (DM) patients with late-onset Fuchs' corneal dystrophy (FCD) and to differentiate them from nonspecific pseudophakic bullous keratopathy (PBK).Human DM-endothelial cell complexes were obtained FCD (n = 40), PBK 6), control eyes 5). Gene expression profiles endothelial cells compared using a commercial real-time PCR array quantitative assays for confirmation differentially expressed genes. A total 24 also...
Clinical features of Coronavirus disease 2019 (COVID-19) are caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Acute infection management is a substantial healthcare issue, and the development long-Covid (LCS) extremely challenging for patients physicians. It associated with variety characteristics as impaired capillary microcirculation, chronic fatigue (CFS), proinflammatory cytokines, functional autoantibodies targeting G-protein-coupled receptors (GPCR-AAbs). Here,...
Rho-associated kinase (ROCK) inhibitors have gained popularity as novel treatment options in the management of glaucoma and corneal endothelial disorders. Among various ocular side effects, reticular epithelial edema has been most frequently reported, mainly after with netarsudil. To explain potential mechanisms, we comparatively analyzed effects ripasudil netarsudil on function vitro. Primary human cells were incubated dihydrochloride hydrochloride dihydrate for up to 7 days. Gene protein...
To determine the role of extracellular chaperone clusterin in pathophysiology pseudoexfoliation (PEX) syndrome/glaucoma, which is characterized by stable deposition abnormal fibrillar material anterior segment tissues.Real-time PCR, situ hybridization, and immunohistochemistry were applied to analyze mRNA protein expression PEX eyes patients without with glaucoma compare them primary open-angle angle-closure normal control eyes. Aqueous levels determined Western blot analysis. Real-time PCR...
The molecular events responsible for obstruction of aqueous humor outflow and the loss retinal ganglion cells in glaucoma, one main causes blindness worldwide, remain poorly understood. We identified a synonymous variant, c.765C>T (Thr255Thr), ankyrin repeats suppressor cytokine signaling box-containing protein 10 (ASB10) large family with primary open angle glaucoma (POAG) mapping to GLC1F locus. This variant affects an exon splice enhancer site alters mRNA splicing lymphoblasts affected...
Although lysyl oxidase-like 1 (LOXL1) is known as the principal genetic risk factor for pseudoexfoliation (PEX) syndrome, a major cause of glaucoma and cardiovascular complications, no functional variants have been identified to date. Here, we conduct genome-wide association scan on 771 German PEX patients 1,350 controls, followed by independent testing associated in Italian Japanese data sets. We focus 3.5-kb four-component polymorphic locus positioned spanning introns 2 LOXL1 with...
Abstract Understanding transcription factor (TF) regulation of limbal epithelial stem/progenitor cells (LEPCs) may aid in using non-ocular to regenerate the corneal surface. This study aimed identify and characterize TF genes expressed specifically LEPCs isolated from human donor eyes by laser capture microdissection. Using a profiling approach, preferential expression was found for SoxE SoxF genes, particularly Sox9, which showed predominantly cytoplasmic localization basal nuclear...
Limbal melanocytes (LMel) represent essential components of the corneal epithelial stem cell niche and are known to protect limbal stem/progenitor cells (LEPCs) from UV damage by transfer melanosomes. Here, we explored additional functional roles for LMel in homeostasis, immune regulation angiostasis. Human corneoscleral tissues were morphologically analyzed normal, inflammatory wound healing conditions. The effects on LEPCs direct indirect co-culture models using electron microscopy,...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes disease 2019 (COVID-19), affects the pulmonary systems via angiotensin-converting enzyme-2 (ACE-2) receptor, being an entry to systemic infection. As COVID-19 features ACE-2 deficiency, a link microcirculation is proposed. Optical coherence tomography angiography (OCT-A) enables non-invasive analysis of retinal microvasculature. Thus, impaired might be mapped on capillary system. recent OCT-A studies, analyzing in two...