A5054543552- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Fungal Biology and Applications
- Bioactive Compounds and Antitumor Agents
- Phytochemical compounds biological activities
- Cell Adhesion Molecules Research
- Regulation of Appetite and Obesity
- Natural product bioactivities and synthesis
- Phytochemistry and Bioactivity Studies
- Autophagy in Disease and Therapy
- Receptor Mechanisms and Signaling
- Phytochemistry and Biological Activities
- Biochemical Analysis and Sensing Techniques
- Natural Compound Pharmacology Studies
- Phytochemicals and Antioxidant Activities
- Science Education and Perceptions
- RNA Interference and Gene Delivery
- Essential Oils and Antimicrobial Activity
- Synthesis of Organic Compounds
- Carbohydrate Chemistry and Synthesis
- Marine Sponges and Natural Products
- Plant-Derived Bioactive Compounds
- Nanoplatforms for cancer theranostics
- Herbal Medicine Research Studies
- Statistics Education and Methodologies
Universidade do Porto
2013-2025
Universidade Federal do Rio Grande do Sul
2025
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2022
University of Rochester
2016
(1) Background: Our previous studies unveiled the hit thioxanthone TXA1 as an inhibitor of P-glycoprotein (drug efflux pump) and human tumor cells growth, namely melanoma cells. Since is structurally similar to lucanthone (an autophagy apoptosis inducer) N10-substituted phenoxazines (isosteres thioxanthones, inducers), this study aimed at further assessing its cytotoxic mechanism evaluating potential modulator in A375-C5 cells; (2) Methods: Flow cytometry with propidium iodide (PI) for cell...
Ganoderma lucidum is one of the most extensively studied mushrooms as a functional food and chemopreventive agent due to its recognized medicinal properties. Some G. extracts have shown promising antitumor potential. In this study, bioactive properties various lucidum, from both fruiting body spores, were investigated. The potent extract identified was methanolic extract, which inhibited growth gastric cancer cell line (AGS) by interfering with cellular autophagy cycle.
Cordyceps militaris (L.) Link, an edible entomopathogenic fungus widely used in traditional Chinese medicine, has numerous potential medicinal properties including antitumor activity. The methanolic extract of C. fruiting body was recently shown to have tumor cell growth inhibitory activity several human lines. Nonetheless, the mechanism action involved is still not known. This work aimed at further studying effect regarding its action, using non-small lung cancer line (NCI-H460) as a model....
Our previous work has described a library of thioxanthones designed to have dual activity as P-glycoprotein modulators and antitumor agents. Some these compounds had shown significant cell growth inhibitory towards leukemia lines, without affecting the non-tumor human fibroblasts. However, their effect in lines derived from solid tumors not been previously studied. The present aimed at: (i) screening this small series an in-house library, for vitro tumor tumors; (ii) initiate study most...
Multidrug resistance (MDR) is one of the main limitations cancer treatment. The overexpression drug-efflux pumps, such as P-glycoprotein (P-gp), a major cause MDR. Importantly, different studies have shown that extracellular vesicles (EVs) participate in communication between MDR cells and drug-sensitive counterparts, promoting dissemination phenotype. In present work, we aimed to identify RNA species EVs released by those cells, which may be associated with content from two pairs (leukemia...
Background: Heat shock protein 90 (HSP90) is a well-known target for cancer therapy. In previous work, some of us have reported series 3-aryl-naphtho[2,3-d]isoxazole-4,9-diones as inhibitors HSP90. Methods: the present various compounds with new chromenopyridinone and thiochromenopyridinone scaffolds were synthesized potential HSP90 inhibitors. Their binding affinity to was studied in vitro. Selected (5 8) further tumor cell lines regarding their cause growth inhibition, alter cycle profile,...
The search for novel anticancer small molecules and strategies remains a challenge. Our previous studies have identified TXA1 (1-{[2-(diethylamino)ethyl]amino}-4-propoxy-9H- thioxanthen-9-one) as hit compound, with in vitro antitumor potential by modulating autophagy apoptosis human tumor cell lines. In the present study, mechanism of action soluble salt this molecule (TXA1.HCl) was further investigated using mouse xenograft models NSCLC. results showed that TXA1.HCl affected steroid...
To date, there are no simple and minimally invasive methods to diagnose MDR. Extracellular vesicles (EVs) shed by all cells, carry a specific cargo from the donor cells present in several body fluids, which means that they can potentially be easily collected cancer patients become source of biomarkers cancer. This data article contains full list proteins identified EVs an isogenic pair chronic myeloid leukaemia (MDR their drug-sensitive counterparts) LC/MS/MS analysis, together with...
Cancer multidrug resistance (MDR) is one of the main challenges for cancer treatment efficacy. MDR a phenomenon by which tumor cells become resistant to several unrelated drugs. Some studies have previously described important role extracellular vesicles (EVs) in dissemination phenotype. EVs’ cargo may include different players MDR, such as microRNAS and drug-efflux pumps, be transferred from donor recipient drug-sensitive counterparts. The present work aimed to: (i) compare ability their...
ALIX [ALG-2 (apoptosis-linked gene 2)-interacting protein X] is one of the most well-known molecular biomarkers extracellular vesicles. Extracellular vesicles are very small released by cells and carry in their cargo components from donor cells, thus being potent vehicles intercellular (horizontal) communication, influencing various physiological pathological functions both recipient cells. The increasing interest highlights key importance a reliable analysis this protein. However, several...