A5072018144- Mesenchymal stem cell research
- Liver physiology and pathology
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Osteoarthritis Treatment and Mechanisms
- Liver Disease Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
- Cancer-related molecular mechanisms research
- Metastasis and carcinoma case studies
- Helicobacter pylori-related gastroenterology studies
- Periodontal Regeneration and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- MicroRNA in disease regulation
- Kruppel-like factors research
- Cancer Genomics and Diagnostics
- Cytokine Signaling Pathways and Interactions
- Bone fractures and treatments
- Cancer Research and Treatments
- Muscle Physiology and Disorders
- dental development and anomalies
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Hormonal Regulation and Hypertension
- Veterinary Orthopedics and Neurology
Georgetown University
2024
Georgetown University Medical Center
2024
VetAgro Sup
2014-2022
Sapienza University of Rome
2006-2014
Università Cattolica del Sacro Cuore
2005-2013
Université Paris Cité
2010-2012
Centre National de la Recherche Scientifique
2010-2012
Inserm
2010-2012
Délégation Paris 5
2012
Institut Cochin
2010-2012
The gene expression pattern of glioblastoma (GBM) is well documented but the profile brain adjacent to tumor not yet analysed. This may help understand oncogenic pathway GBM development. We have established genome-wide profiles samples isolated from mass, white matter (apparently free cells), and controls by using Affymetrix HG-U133 arrays. Array-CGH (aCGH) was also performed detect genomic alterations. Among genes dysregulated in peritumoral matter, 15 were over-expressed, while 42...
Objective: To explore the long-term safety and efficacy of canine allogeneic mesenchymal stromal cells (MSC) administered intra-articularly as single or repeated injections in appendicular joints dogs affected by moderate to severe refractory osteoarthritis. Study design: 22 pet were recruited into a non-randomized, open monocentric study initially administering one cellular injection. A second injection was offered after 6 months owners if first did not produce expected results. Materials...
Due to its abundance, easy retrieval, and plasticity characteristics, adipose-tissue-derived stromal cells (ATSCs) present unquestionable advantages over other adult-tissue-derived stem cells. Based on the in silico analysis of our previous data reporting ATSC-specific expression profiles, study attempted clarify validate at functional level neurospecific genes expressed by ATSC both vitro vivo. This allowed evidencing that ATSCs express neuro-specific trophins, metabolic genes,...
The purpose of this prospective study was to evaluate the effects single and repeated intra-articular administration allogeneic, umbilical cord-derived, neonatal mesenchymal stem cells (MSC) in horses with lameness due osteoarthritis (OA) a metacarpophalangeal joint (MPJ). Twenty-eight were included. Horses divided into two groups. group MSC1 received an MSC injection at M0 placebo M1 (1 month after M0). MSC2 injections M1. Joint performed blinded syringe. Clinical assessment by treating...
Compare the clinical and pressure walkway gait evolution of dogs after a tibial plateau leveling osteotomy (TPLO) for cranial cruciate ligament rupture (CrCLR) treatment with either 1-month course non-steroidal anti-inflammatory drugs (NSAIDs) or single postoperative intra-articular (IA) injection allogeneic neonatal mesenchymal stromal cells (MSCs).Prospective, double-blinded, randomized, controlled, monocentric study.Sixteen client-owned dogs.Dogs unilateral CrCLR confirmed by arthroscopy...
Abstract Background: Previous studies suggested an association between CagA‐positive H. pylori strains and ischemic stroke. The aim of the present study was to assess prevalence Helicobacter infection CagA status in patients with atherosclerotic stroke primary care setting. Materials methods: A total 106 consecutive (age 76.6 ± 8 years; males 52%) well‐documented history sex–age‐ 76.5 9 social background‐matched controls without relevant vascular diseases. Risk factors for were recorded all...
<h3>Objective</h3> To test the osteo-regenerative potential of adipose tissue–derived stromal cells (ATSCs), an attractive human source for tissue engineering, in a rat model mandibular defect. Human dermal fibroblasts (HDFs) were used as differentiated cellular control study. <h3>Design</h3> The ATSCs and HDFs isolated from lipoaspirate skin biopsy specimens, respectively. Cells characterized vitro then adsorbed on osteo-conductive scaffold to be transplanted defect immunosuppressed rats....
As in humans, osteoarthritis (OA) causes considerable economic loss to the equine industry. New hopes for cartilage repair have emerged with matrix-associated autologous chondrocyte implantation (MACI). Nevertheless, its limitation is due dedifferentiation occurring during amplification phase, leading of capacity produce a hyaline extracellular matrix (ECM). To enhance MACI therapy efficiency, we developed strategy redifferentiation, and demonstrated feasibility model. Thus, mimic...
Abstract Umbilical cord blood mesenchymal stromal/stem cells (UCB-MSCs) and umbilical matrix MSCs (UCM-MSCs) have chondrogenic potential are alternative sources to standard surgically derived bone marrow or adipose tissue collection for cartilage engineering. However, the majority of comparative studies explore neonatal only on ISCT benchmark assays accounting some bias in reproducibility between vitro clinical studies. Therefore, we characterized equine UCB-MSCs UCM-MSCs investigated with...
Several in vitro assays have been proposed to identify cancer stem cells (CSCs), including immunophenotyping, sphere assay and side population (SP) assay. CD133 antigen has as a CSC marker colon (CC). However, no functional data are available date conflicting results reported regarding its role true marker. Here we set out molecular signature associated with potential CSC. CD133(+) isolated from the CaCo-2 CC cell line were analysed by microarray profiling compared CD133(-) counterparts....
The mitogen-activated protein kinases (MAPK) ERK1 and ERK2 are among the major signal transduction molecules but little is known about their specific functions in vivo. ERK activity provided by two isoforms, ERK2, which ubiquitously expressed share activators substrates. However, there not vivo studies have reported a role for or HSCs bone marrow microenvironment. present study shows that ERK1-deficient mice mild osteopetrosis phenotype. lodging homing abilities of ERK1−/− HSC impaired,...
Multipotent mesenchymal stem cells with extensive self-renewal properties can be easily isolated and rapidly expanded in culture from small volumes of amniotic fluid. These cells, namely, fluid-stromal (AFSCs), regarded as an attractive source for tissue engineering purposes, being phenotypically genetically stable, plus overcoming all the safety ethical issues related to use embryonic/fetal cells. LMP3 is a novel osteoinductive molecule acting upstream main osteogenic pathways. This study...
Lim mineralization protein-3 (LMP3) induces osteoblast differentiation by regulating the expression and activity of certain molecules involved in osteogenic cascade, including those belonging to bone morphogenetic protein (BMP) family. The complete network molecular events LMP3-mediated osteogenesis is still unknown. aim this study was analyze genome-wide gene profiles human mesenchymal stem cells (hMSC) induced exogenous LMP3 mediate osteogenesis. For purpose hMSC were transduced with a...
Background: The growing interest in mesenchymal stromal cells (MSCs) equine medicine, together with the development of MSC biobanking for allogeneic use, raises concerns about biosafety such products. MSCs derived from umbilical cord (UC) carry an inherent risk contamination by environmental conditions and vertical transmission pathogens broodmares. There is yet no report scientific literature horses being contaminated infected products, consensus systematic infectious screening cord-derived...