A5030706001- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Blood Coagulation and Thrombosis Mechanisms
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Chemokine receptors and signaling
- Fungal and yeast genetics research
- HER2/EGFR in Cancer Research
- Phytochemical compounds biological activities
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Protein Hydrolysis and Bioactive Peptides
- MicroRNA in disease regulation
- Beetle Biology and Toxicology Studies
- RNA modifications and cancer
- Hemophilia Treatment and Research
- Genomics and Chromatin Dynamics
- Ovarian cancer diagnosis and treatment
- Enzyme Production and Characterization
- Biochemical and Structural Characterization
- Circular RNAs in diseases
- Radiopharmaceutical Chemistry and Applications
- Alzheimer's disease research and treatments
Technical University of Munich
2015-2025
Klinikum rechts der Isar
2014-2024
Klinik für Frauenheilkunde
2010-2024
München Klinik
1998-2021
Olgahospital
1995-2015
Ludwig-Maximilians-Universität München
1988-2014
Technische Universität Dresden
1998-2003
Wilex (Germany)
2000-2002
Max Planck Institute of Biochemistry
1993-2000
Asklepios Klinik St. Georg
2000
Extravasation and intravasation of solid malignant tumors is controlled by attachment tumor cells to components the basement membrane extracellular matrix, local proteolysis cell migration. Strong clinical experimental evidence has accumulated that tumor-associated serine protease plasmin, its activator uPA (urokinase-type plasminogen activator), receptor uPA-R (CD87), inhibitors PAI-1 PAI-2 are linked cancer invasion metastasis. In cancer, increase uPA, uPA-R, and/or associated with...
We have isolated profilin from yeast (Saccharomyces cerevisiae) and microsequenced a portion of the protein to confirm its identity; region agrees with predicted amino acid sequence gene recently S. cerevisiae (Magdolen, V., U. Oechsner, G. Müller, W. Bandlow. 1988. Mol. Cell. Biol. 8:5108-5115). Yeast resembles profilins other organisms in molecular mass ability bind polyproline, retard rate actin polymerization, inhibit hydrolysis ATP by monomeric actin. Using strains that carry...
The in vivo roles of meprin metalloproteases pathophysiological conditions remain elusive. Substrates define protease roles. Therefore, to identify natural substrates for human α and β we employed TAILS (terminal amine isotopic labeling substrates), a proteomics approach that enriches N-terminal peptides proteins cleavage fragments. Of the 151 new extracellular identified, it was notable ADAM10 (a disintegrin metalloprotease domain-containing protein 10)-the constitutive α-secretase-is...
Abstract Background Response to immune checkpoint blockade (ICB) in ovarian cancer remains disappointing. Several studies have identified the chemokine CXCL9 as a robust prognosticator of improved survival and characteristic immunoreactive subtype, which predicts ICB response. However, function has been poorly studied. Methods Impact Cxcl9 overexpression murine ID8- Trp53 −/− −/– Brca2 models on survival, cellular composition, PD-L1 expression anti-PD-L1 therapy. analysis subtypes...
Human tissue kallikreins (hKs) form a family of 15 closely related (chymo)trypsin-like serine proteinases. These are expressed in wide range tissues including the central nervous system, salivary gland, and endocrine-regulated tissues, such as prostate, breast, or testis, may have diverse physiological functions. For several kallikreins, clear correlation has been established between expression different types cancer. example, prostate-specific antigen (PSA hK3) serves tumor marker is used...
The glycosylphosphatidylinositol (GPI)-anchored, multifunctional receptor for the serine proteinase, urokinase plasminogen activator (uPAR, CD87), regulates activation and cell migration, adhesion, proliferation. uPAR occurs in functionally distinct, membrane-anchored soluble isoforms (s-uPAR) vitro vivo. Recent evidence indicates that s-uPAR present circulation of cancer patients correlates with tumor malignancy represents a valuable prognostic marker certain types cancer. We have therefore...
Epithelial ovarian cancer (EOC) remains a highly-lethal gynecological malignancy, characterized by frequent recurrence, chemotherapy resistance and poor 5-year survival. Identifying novel predictive molecular markers an overdue challenge in the disease's clinical management. Herein, silico analysis of TCGA-OV highlighted tRNA-derived internal fragment (i-tRF-GlyGCC) among most abundant tRFs tumors, while target prediction gene ontology (GO) enrichment predicted its implication key biological...
The gene coding for profilin (PFY), an actin-binding protein, occurs as a single copy in the haploid genome of Saccharomyces cerevisiae and is required spore germination cell viability. Displacement one diploid by nonfunctional allele recessively lethal: tetrad analysis yields only two viable spores per ascus. PFY maps on chromosome XV linked to ADE2 marker. primary transcript about 1,000 bases contains intron 209 spliced into messenger 750 bases. was identified comparison with cDNA clone,...
The human tissue kallikrein family of serine proteases (hK1-hK15 encoded by the genes KLK1-KLK15) is involved in several cancer-related processes. Accumulating evidence suggests that certain kallikreins are part an enzymatic cascade pathway activated ovarian cancer and other malignant diseases. In present study, OV-MZ-6 cells were stably co-transfected with plasmids expressing hK4, hK5, hK6, hK7. These displayed similar proliferative capacity as vector-transfected control (which do not...
hK7 or human stratum corneum chymotryptic enzyme belongs to the tissue kallikrein (hKs) serine proteinase family and is strongly expressed in upper layers of epidermis. It participates skin desquamation but also implicated diverse diseases a potential biomarker ovarian cancer. We have solved x-ray structures recombinant active at medium atomic resolution presence inhibitors succinyl-Ala-Ala-Pro-Phe-chloromethyl ketone Ala-Ala-Phe-chloromethyl ketone. The most distinguishing features are...
The metalloproteases meprin alpha and beta are expressed in several tissues, leukocytes, cancer cells. In skin, meprins located separate layers of human epidermis indicating distinct physiological functions, supported by effects on cultured keratinocytes. Meprin induces a dramatic change cell morphology significant reduction number, whereas vitro evidence suggests role for basal keratinocyte proliferation. Meprins secreted as zymogens that activated tryptic proteolytical processing. Here, we...
Kallikrein‐related peptidases, in particular KLK4, 5, 6 and 7 (4–7), often have elevated expression levels ovarian cancer. In OV‐MZ‐6 cancer cells, combined of KLK4–7 reduces cell adhesion increases invasion resistance to paclitaxel. The present work investigates how shape the secreted proteome (“secretome”) proteolytic profile (“degradome”) cells. secretome comparison consistently identified >900 proteins three replicate analyses. Expression predominantly affected abundance involved...
The serine protease uPA (urokinase-type plasminogen activator) and its receptor uPAR (CD87) are often elevated in malignant tumours, hence, inhibition of this tumour-associated activation system provides an attractive target for therapeutic strategies. WX-UK1, a derivative 3-aminophenylalanine the L-conformation with inhibitory antiproteolytic properties, was tested specificity spectrum using specific chromogenic paranitroanilide peptide substrates. corresponding D-enantiomer WX-UK1 used as...