Dinoflagellates are an important component of the marine biota, but a large genome with high–copy number (up to 5,000) tandem gene arrays has made genomic sequencing problematic. More importantly, little is known about expression and conservation these unusual arrays. We assembled de novo catalog 74,655 contigs for dinoflagellate Lingulodinium polyedrum from RNA-Seq (Illumina) reads. The contains 93% EST dataset deposited in GenBank 94% enzymes 16 primary metabolic KEGG (Kyoto Encyclopedia...

Background Dinoflagellates typically lack histones and nucleosomes are not observed in DNA spreads. However, recent studies have shown the presence of core histone mRNA sequences scattered among different dinoflagellate species. To date, all components required for manufacturing modifying a single species has been confirmed. Methodology Results Analysis Lingulodinium transcriptome obtained by Illumina sequencing shows several copies each four as well suite enzymes chaperone proteins....

Abstract Dinoflagellates are microscopic, eukaryotic, and primarily marine plankton. Temporary cyst formation is a well-known physiological response of dinoflagellate cells to environmental stresses. However, the molecular underpinnings cold-induced physiology have never been described. Cultures photosynthetic Lingulodinium polyedrum readily form temporary cysts when placed at low (8°C ± 1°C) temperature excyst normal motile following return (18°C 1°C). The circadian bioluminescence rhythm...

Almost all cells display circadian rhythms, approximately 24-hour period changes in their biochemistry, physiology or behavior. These rhythms are orchestrated by an endogenous clock whose mechanism is based on transcription-translation feedback loops (TTFL) where the translated products of genes act to inhibit own transcription. We have used RNA-Seq measure abundance transcripts RNA-Seq-derived de novo gene catalog two different experiments. One compared midday and midnight a light–dark...

ABSTRACT Circadian clocks execute temporal regulation of metabolism by modulating the timely expression genes. Clock mRNA synthesis was envisioned as primary driver these daily rhythms. oscillations often do not concur with downstream protein oscillations, revealing importance to study oscillations. Chlamydomonas reinhardtii is a well‐studied miniature plant model. We quantitatively probed proteome for two subsequent circadian cycles using high throughput SWATH‐DIA mass spectrometry....

Roughly two-thirds of the proteins annotated as transcription factors in dinoflagellate transcriptomes are cold shock domain-containing (CSPs), an uncommon condition eukaryotic organisms. However, no functional analysis has ever been reported for a CSP, and so it is not known if they do fact act factors. We describe here some properties two CSPs from Lingulodinium polyedrum, LpCSP1 LpCSP2, which contain glycine-rich C-terminal domain N-terminal phylogenetically related to those bacteria....

Pregnenolone (P5) promotes prostate cancer cell growth, and de novo synthesis of intratumoural P5 is a potential cause development castration resistance. Immune cells can also synthesize novo. Despite its biological importance, little known about P5's mode actions, which appears to be context dependent pleiotropic. A comprehensive proteome-wide spectrum P5-binding proteins that are involved in trafficking functionality remains unknown. Here, we describe an approach integrates chemical...

Pregnenolone (P5) is synthesized as the first bioactive steroid in mitochondria from cholesterol. Clusters of differentiation 4 (CD4+) and 8 (CD8+) immune cells synthesize P5 de novo; P5, turn, play important role homeostasis regulation. However, P5's biochemical mode action still emerging. We envisage that revealing complete spectrum target proteins would have multifold applications, not only basic understanding steroids biochemistry but also developing new therapeutic applications....

Abstract Pregnenolone (P5) promotes prostate cancer cell growth, and de novo synthesis of intratumoural P5 is a potential cause development castration-resistance. Immune cells can also synthesize . Despite its biological importance, little known about P5’s mode actions, which appears to be context-dependent pleiotropic. A comprehensive proteome-wide spectrum P5-binding proteins that are involved in trafficking functionality remains unknown. Here, we describe an approach integrates chemical...

Abstract Pregnenolone (P5) is synthesized as the first bioactive steroid in mitochondria from cholesterol. CD4+ and CD8+ immune cells synthesize P5 de novo , turn play important role homeostasis regulation. However, P5’s biochemical mode of action still emerging. We envisage that revealing complete spectrum P5-target proteins would have multifold applications, not only basic understanding steroids biochemistry but also developing new therapeutic applications. employed a CLICK-enabled probe...

Abstract Timing of biological processes enable organisms to sustain the diurnal fluctuations resulting from earth’s rotation. Circadian clocks execute this temporal regulation by modulating expression genes. Clock mRNAs was envisioned as primary driver daily rhythms. However, mRNA oscillations often don’t concur with downstream protein oscillations. To assess contribution post-transcriptional processes, we quantitatively probed Chlamydomonas proteome for two circadian cycles. Our study...

HUMAN JURKAT T LYMPHOCYTES. K. Gohil, S. Roy. Khanna L. Packer and C.K. Sen Department of Molecular Cell Biologq, Universi3 California. Berkele?, CA. USA a-Lipolc acid (LA) is a thiol antioxidant which influences cellular redox ststus. We have demonstrated an inhibitory role LA in agonist induced NFKB activation. To obtain comprehensive understanding the effect on gene expression profile we applied technique dlfi’erential di\pla! iDD) mRNAs. RNA from human Jurkat cell treated Lvith either (...