A5058922935- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Pneumonia and Respiratory Infections
- Microbial bioremediation and biosurfactants
- Microbial Community Ecology and Physiology
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Identification and Quantification in Food
- Mycobacterium research and diagnosis
- Infective Endocarditis Diagnosis and Management
- Plant Pathogenic Bacteria Studies
- Vibrio bacteria research studies
- Bacterial Infections and Vaccines
- Microbial infections and disease research
- Microbial Natural Products and Biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Tuberculosis Research and Epidemiology
- Plant-Microbe Interactions and Immunity
- Probiotics and Fermented Foods
- Urinary Tract Infections Management
- Microbial Applications in Construction Materials
- Infections and bacterial resistance
University of Gothenburg
2016-2025
Sahlgrenska University Hospital
2018-2025
RISE Research Institutes of Sweden
2025
Region Västra Götaland
2020-2024
Universitat de les Illes Balears
2016-2024
Institute for Biomedicine
2022
of the Marmur procedure.
We present the first complete, closed genome sequences of Streptococcus pyogenes strains NCTC 8198
When analysing a large cohort of Staphylococcus haemolyticus , using whole-genome sequencing, five human isolates (four from the skin and one blood culture) with aberrant phenotypic genotypic traits were identified. They phenotypically similar yellow colonies, nearly identical 16S rRNA gene sequences initially speciated as S. based on sequence MALDI-TOF MS. However, compared to these strains demonstrate: (i) considerable phylogenetic distance an average nucleotide identity <95 % inferred...
A range of methodologies may be used for analyzing bacteria, depending on the purpose and level resolution needed. The capability recognition species distinctions within complex spectrum bacterial diversity is necessary progress in microbiological research. In clinical settings, accurate, rapid cost-effective methods are essential early efficient treatment infections. Characterization identification microorganisms, using, bottom-up proteomics, or “proteotyping”, relies species-unique...
Alcaligenes aquatilis QD168 is a marine, aromatic hydrocarbon-degrading bacterium, isolated from an oil-polluted sediment of Quintero Bay, industrial-coastal zone that has been chronically impacted by diverse pollutants. The aims this study were to characterize the phylogenomic positions spp. and genetic determinants physiological response A. model environmental stressors (benzene, oxidizing agents, salt). Phylogenomic analyses, using 35 housekeeping genes, clustered with four other strains...
Mass spectrometry (MS) and proteomics offer comprehensive characterization identification of microorganisms discovery protein biomarkers that are applicable for diagnostics infectious diseases. The use is widely applied in the clinic peptide increasingly being investigated applications clinical laboratory. Respiratory-tract infections a predominant cause medical treatment, although, assessments standard laboratory protocols time-consuming often inadequate reliable diagnoses. Novel methods,...
Population-based sewage surveillance has emerged as a promising approach for studying the prevalence of antibiotic resistance in pathogens.
Pseudomonas aeruginosa is an opportunistic pathogen with increasing incidence of multidrug-resistant strains, including resistance to last-resort antibiotics, such as carbapenems. Resistances are often due complex interplays natural and acquired mechanisms that enhanced by its large regulatory network. This study describes the proteomic responses two carbapenem-resistant P. strains high-risk clones ST235 ST395 subminimal inhibitory concentrations (sub-MICs) meropenem identifying...
Three β-lactam resistant bacterial strains isolated from gut samples of wild Atlantic mackerel ( Scomber scombrus ) collected the northern North Sea were characterized by polyphasic analyses. The determined to belong genus Pseudomonas but could not be assigned a known species. nearly-complete 16S rRNA gene sequence showed highest similarity (99.9%) four different species, although partial rpoD exhibited relatively low similarities proteolytica (93.4%) and other spp. Genome sequencing...
The family Enterobacteriaceae is a taxonomically diverse and widely distributed containing many human commensal pathogenic species that are known to carry transferable antibiotic resistance determinants. Characterization of novel taxa within this great importance in order understand the associated health risk provide better treatment options. aim present study was characterize Gram-negative bacterial strain (CCUG 66741) belonging Enterobacteriaceae, isolated from wound infection an adult...
Correct identifications of isolates and strains the Mitis-Group genus Streptococcus are particularly difficult, due to high genetic similarity, resulting from horizontal gene transfer homologous recombination, unreliable phenotypic genotypic biomarkers for differentiating species. pneumoniae pseudopneumoniae most closely related species clade. In this study, publicly-available genome sequences S. were analyzed, using a pangenomic approach, find candidates species-unique markers; ten genes...
Abstract Background Environmental surveillance of antibiotic resistance can contribute towards better understanding and management human environmental health. This study applied a combination long-read Oxford Nanopore MinION short-read Illumina MiSeq-based sequencing to obtain closed complete genome sequences two CTX-M-producing multidrug-resistant Escherichia coli strains isolated from blue mussels ( Mytilus edulis ) in Norway, order understand the potential for mobility detected genes...
The genus Clavibacter has been associated largely with plant diseases. aims of this study were to characterize the genomes and virulence factors Chilean C. michiganensis subsp. strains VL527, MSF322 OP3, define their phylogenomic positions within species, michiganensis. VL527 possess 3,396,632 3,399,199 bp, respectively, a pCM2-like plasmid in strain pCM1- plasmids MSF322. OP3 genome is composed chromosome three (including plasmids) 3,466,104 bp. Genomic analyses confirmed phylogenetic...
Correct serotype identification of Streptococcus pneumoniae (pneumococcus) is important for monitoring disease epidemiology and assessing the impacts pneumococcal vaccines. Furthermore, correct differentiation pathogenic S. from closely related commensal species mitis group genus are essential identification.A new protocol determining existing 98 serotypes pneumococcus was developed, applying two PCR amplifications amplicon sequencing, using newly designed internal primers. The validated...
The taxonomic status of six strains Acinetobacter obtained from meat samples, collected supermarkets in Porto, Portugal, was investigated using polyphasic analysis. Partial rpoB sequence similarities lower than 95 % to other species with validly published names led the hypothesis that these represented novel species. This confirmed based on comparative multilocus analysis, which included gyrB , recA and 16S rRNA genes, revealing two coherent lineages were distinct each all known portensis...
Stutzerimonas balearica (Pseudomonas balearica) has been found principally in oil-polluted environments. The capability of S. to thrive from the degradation pollutant compounds makes it a species interest for potential bioremediation applications. However, little reported about diversity balearica. In this study, genome sequences strains different origins were analyzed, revealing that is diverse with an open pan-genome will continue new genes and functionalities as genomes more are...
Bloodstream infections (BSIs), the presence of microorganisms in blood, are potentially serious conditions that can quickly develop into sepsis and life-threatening situations. When assessing proper treatment, rapid diagnosis is key; besides clinical judgement performed by attending physicians, supporting microbiological tests typically performed, often requiring microbial isolation culturing steps, which increases time required for confirming positive cases BSI. The additional waiting...
Exploring Brevibacterium strains from various ecosystems may lead to the discovery of new antibiotic-producing strains. sp. H-BE7, a strain isolated marine sediments Northern Patagonia, Chile, had its genome sequenced study biosynthetic potential produce novel natural products within genus. The sequences 98 strains, including were selected for genomic analysis. A phylogenomic cladogram was generated, which divided into four major clades. total 25 are potentially unique species according...
Alcaligenes aquatilis strain QD168 (= CCUG 69566) is a marine hydrocarbon-degrading bacterium isolated from crude oil-polluted sediment Quintero Bay, Central Chile. Here, we present the 4.32-Mb complete genome sequence of QD168, with 3,892 coding sequences, 58 tRNAs, and 56.3% G+C content.
We identified two new plasmids in Shewanella baltica isolated from wild Atlantic mackerel ( Scomber scombrus ) collected the northern North Sea, one plasmid carrying mcr-4.3 gene for colistin resistance and operon merRPAT mercury other multiple heavy metal genes. The marine environment has been recognized as a source of genes that are found human pathogens.
Fast and accurate identifications of pathogenic bacteria along with their associated antibiotic resistance proteins are paramount importance for patient treatments public health. To meet this goal from the mass spectrometry aspect, we have augmented previously published Microorganism Classification Identification (MiCId) workflow capability. evaluate performance workflow, used MS/MS datafiles samples 10 bacterial strains belonging to three different species: Escherichia coli, Klebsiella...
Clavibacter michiganensis subsp. (Cmm) is the causal agent of bacterial canker tomato. Differences in virulence between Cmm strains have been reported. The aim this study was characterization nine isolated Chile to reveal causes their differences virulence. assays tomato seedlings revealed different levels severity associated with strains, two highly virulent and one causing only mild symptoms. most showed increased cellulase activity, no activity observed strain In three including PCR...