A5043996734- Mosquito-borne diseases and control
- Research on Leishmaniasis Studies
- Insect Pest Control Strategies
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Trypanosoma species research and implications
- Malaria Research and Control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Zoonotic diseases and public health
- Viral Infections and Outbreaks Research
- Dengue and Mosquito Control Research
- Insect behavior and control techniques
- Entomopathogenic Microorganisms in Pest Control
- Fungal Biology and Applications
- Diptera species taxonomy and behavior
- Psychodrama and Leishmaniasis Studies
- Parasitic Infections and Diagnostics
- Essential Oils and Antimicrobial Activity
- Hymenoptera taxonomy and phylogeny
- Viral-associated cancers and disorders
- Global Maternal and Child Health
- T-cell and Retrovirus Studies
- Plant Virus Research Studies
- Complement system in diseases
Universidade Federal do Paraná
2014-2024
Institut Pasteur
2007
Fundação Oswaldo Cruz
2002-2006
In this study, we detected Leishmania (Viannia) braziliensis infection in equids living endemic regions of cutaneous leishmaniasis. To determine the role these animals cycle, used two approaches: serological and molecular methods. Antibodies to parasite were assayed using Enzyme Linked Immunosorbent Assay (ELISA). Blood samples collected tested by polymerase chain reaction (PCR), positive products sequenced. The results showed that 11.0% (25/227) seropositive for sp, 16.3% (37/227) PCR...
BackgroundCYD-TDV (Dengvaxia®) was the first dengue vaccine approved, launched in Brazil 2015 for individuals aged 9–44 years. We aimed to estimate effectiveness of CYD-TDV preventing symptomatic cases during a campaign targeting 15–27 years selected municipalities Paraná, Brazil. Additionally, we examined whether history dengue, as recorded by surveillance system, modified vaccine's effectiveness.MethodsWe conducted case-cohort analysis comparing frequency vaccination, with at least one...
The feeding behavior of sand flies provides valuable information about the vector/host interactions and elucidates epidemiological patterns American cutaneous leishmaniasis (ACL) transmission. aim this study was to identify blood meal sources in endemic areas Paraná State through polymerase chain reaction (PCR) amplification a prepronociceptin (PNOC) gene fragment its subsequent DNA sequencing. Moreover, molecular assays were conducted evaluate sensitivity reproducibility PNOC amplification....
Aedes aegypti populations from five districts in Rio de Janeiro were analyzed using microsatellites and six isoenzyme markers, to assess the amount of variation patterns gene flow at local levels. Microsatellite loci polymorphic enough detect genetic differentiation collected small geographic scales (e.g. within a city). Ae. highly differentiated as well city center outskirt. Thus, dengue virus propagation by mosquitoes could be efficient urban area outskirt Janeiro, main entry point Brazil.
We present a population genetic study of Aedes aegypti in Brazil using isoenzyme markers. Four polymorphic loci were used to examine 11 mosquito collections at four periods 2003. Samples from dengue-endemic area (southeastern region) and dengue-free (southern connected by an important network roads railways analyzed. The degree differentiation observed between populations is consistent with limited gene flow them. There was no evidence passive dispersion Ae. vehicles among the different...
Aedes aegypti and albopictus are considered the most important vectors of arboviruses in world. is primary vector dengue, urban yellow fever, chikungunya zika Brazil, Ae. a potential vector. Distribution patterns influence climatic variables on oviposition were evaluated Morretes, tourist city coastal area Paraná State, which has recently been experiencing cases dengue fever. Eggs collected using ovitraps over period one year (September 2017 to September 2018) reared from hatching until...
Introduction The blood meal source of sandflies provides valuable information about the vector/host interaction and allows for an understanding American cutaneous leishmaniasis (ACL) transmission mechanisms. aim this study was to identify sources Lutzomyia (Nyssomyia) intermedia in endemic area Brazil's State Paraná using a precipitin test. Methods Sandflies were collected rural locality Epitácio Pessoa within City Adrianópolis, Paraná, southern Brazil. A total 864 female captured, 862...
BACKGROUND The incidence of visceral leishmaniasis (VL) has increased in the Southern region Brazil recent years, especially State Paraná. New species have been suggested with potential to act as vector VL endemic areas. OBJECTIVES Identify Leishmania sand fly specimens collected from 2016 2018 municipality Itaperuçu, Vale do Ribeira, Paraná, Brazil. METHODS Light traps were used for collections and analysis multiplex polymerase chain reaction (PCR) methodology subsequent sequencing....
Rio de Janeiro is considered as the most important entry point for dengue viruses in Brazil. Using isoenzyme markers, we investigated genetic structure of mosquito vector Aedes aegypti sampled at three-month intervals 14 districts from December 2002 to 2003. We detected high levels differentiation (i.e. F(ST) values and significant P values), which tended persist throughout year. The species does not take advantage routes railways disperse. Genetic structuring was higher rainy season,...
Environmental changes have occurred over the years, altering eco-epidemiological pattern of leishmaniosis in State Paraná, Brazil, involving pillars cycle (parasite, vectors, reservoir, and environment) their interaction. Much has been discussed about dog's role as a reservoir Leishmania (Viannia) braziliensis Vianna, 1911 transmission cycle. However, this question remains unanswered. The purpose study was to investigate, using parasitological molecular methods, different samples eight...
The success of vaccination programs depends on the level acceptance vaccine to achieve high coverage rates (VCR). Vaccine hesitancy is a challenge, especially concerning new vaccines. Dengue vaccine, Dengvaxia®, was licensed in Brazil 2015 and implemented, pioneering publicly-funded initiative state Paraná, between 2016 2018. program took place five phases 30 municipalities most affected by dengue state, targeting individuals from nine 44 years-old two cities 15 27 other 28 municipalities,...
American tegumentary leishmaniasis (ATL) is highly endemic in the Amazon basin and occurs all South countries, except Chile Uruguay. Most Brazilian ATL cases are due to Leishmania (Viannia) braziliensis , however other neglected Amazonian species being increasingly reported. They belong subgenus L . information on suitable models understand immunopathology scarce. Here, we explored use of golden hamster Mesocricetus auratus its macrophages as a model for species. We also studied interaction...
The diagnosis and incidence of spinocerebelar ataxias (SCA) is sometimes difficult to analyze due the overlap phenotypes subtypes are disorders mutations caused by CAG trinucleotide repeat expansion. To investigate SCA in Southern Brazil, we analyzed repeats (CAG)n at SCA1, SCA2, SCA3, SCA6 SCA7 loci identify allele size ranges frequencies. We examined blood sample from 154 asymptomatic donors 115 individuals with progressive ataxias. PCR products were submitted capillary electrophoresis. In...
Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinae sub-family and belongs to genus Rhadinovirus . This virus has been associated with different clinical manifestations research activity put forward strong correlation among infection, postpartum metritis, abortion. The goal this work was characterize strain isolate from cow's uterine outflow. From swabs drawn secretion, isolated characterized by its cytopathology, morphology, molecular biology approaches. In culture there CPE...
Delia sanctijacobi is critically assessed and given a revised description using data from scanning electronic microscopy (SEM) DNA barcode analysis. This species recorded for the first time in Brazil Peru. We provide morphological identification key (with figures) Brazil, molecular based on COI (cytochrome C oxidase subunit I) sequences an updated distributional map. also report occurrence of D. feeding Brassica Brazil. potential pest was observed broccoli roots (Brassica oleracea var....
A Leishmaniose Tegumentar Americana (LTA) é caracterizada por feridas na pele em forma de “cratera lua” com baixa mortalidade, mas alta morbidade. Embora exista diagnóstico e tratamento para a LTA, doença requer que seja feito um trabalho educativo prevenção, uma vez o muito invasivo agressivo. Considerando os elevados números casos autóctones LTA região do Vale Ribeira, objetivo desse estudo foi realizar ações formativas educativas nos municípios Cerro Azul Itaperuçu anos 2016 2019. Desse...