A5040884138- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- RNA modifications and cancer
- Virus-based gene therapy research
- Reproductive System and Pregnancy
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Vectors
- Clostridium difficile and Clostridium perfringens research
- CRISPR and Genetic Engineering
- Anodic Oxide Films and Nanostructures
- Phagocytosis and Immune Regulation
- Circular RNAs in diseases
- Immune Response and Inflammation
- Lipid Membrane Structure and Behavior
Indian Institute of Science Bangalore
2024-2025
Indian Institute of Chemical Biology
2016-2022
Council of Scientific and Industrial Research
2022
microRNAs (miRNAs), the tiny but stable regulatory RNAs in metazoan cells, can undergo selective turnover presence of specific internal and external cues to control cellular response against changing environment. We have observed reduction miR-122 content, due their accelerated extracellular export human hepatic cells starved for small metabolites including amino acids. In this context, a new role ELAV protein HuR has been identified. HuR, negative regulator miRNA function, accelerates...
Targeted delivery of diagnostic and therapeutic agents to tumor microenvironments using nanoparticles improves the efficacy these reduces their unwarranted side effects. Numerous synthetic nanoparticle systems have been designed for this very purpose, but few translated clinically due poor efficacy-to-cost efficacy-to-toxicity ratios. Biological such as modified extracellular vesicles (EVs) that are likely lower toxicities also developed face challenges in clinical translation they primarily...
MicroRNAs (miRNAs), the tiny regulators of gene expression, can be transferred between neighbouring cells via extracellular vesicles (EVs) to control expression genes in both donor and recipient cells. How EV-derived miRNAs are internalized become functional target is an unresolved question. We have expressed a liver-specific miRNA, miR-122, non-hepatic for packaging released EVs. With these EVs, we followed trafficking miR-122 HeLa that otherwise do not express this miRNA. found...
Leishmania donovani , the causative agent of visceral leishmaniasis, infects and resides within tissue macrophage cells. It is not clear how parasite infected cells crosstalk with noninfected to regulate infection process. During infection, adopts a dual strategy for its survival by regulating intercellular transport host miRNAs restrict inflammation. The parasite, preventing mitochondrial function cells, restricts entry liver cell derived miR-122–containing extracellular vesicles in...
MicroRNAs (miRNAs) are small regulatory RNAs of relatively long half-life in non-proliferative human cells. However, cancer cells the half-lives miRNAs comparatively short. To understand mechanism rapid miRNA turnover cells, we explored effect target mRNAs on abundance that repress them. We have noted an accelerated extracellular vesicle (EV)-mediated export presence their mammalian and this target-driven miRNA-export process is retarded by Ago2-interacting protein GW182B. The GW182 group...
Base modification and the use of lipid nanoparticles (LNPs) are thought to be essential for efficient in vivo delivery expression mRNA. However, ex immune cell engineering, need either two is unclear. Previous reports have suggested that nucleic acids may efficiently delivered cells vivo, through a non-endosomal route, but base has not been determined. Herein, we demonstrate when method used, unmodified mRNA performs equally well commonly used base-modified mRNA, including N1 methyl...
Base modification and the use of lipid nanoparticles are thought to be essential for efficient in vivo delivery expression mRNA. However, ex immune cell engineering, need either two is unclear. Previous reports have suggested that nucleic acids may efficiently delivered cells vivo, through a nonendosomal route, but base has not been determined. Herein, we demonstrate when method used, unmodified mRNA performs equally well commonly used base-modified mRNA, including N1 methyl pseudouridine...
Summary The infectious microbe Staphylococcus aureus releases an array of cytotoxic pore-forming toxins (PFTs) that severely damage the cell membrane during bacterial infection. However, interaction interfaces between host and toxin were merely explored. Herein, we monitored active oligomeric states facilitated disruption processes such as lysis, protrusion in plasma lipid membrane. Furthermore, necrosis was triggered neutrophil-like cells upon synergistic binding oligomerization monomeric...
Abstract MicroRNAs are small regulatory RNAs of relatively long half-life in non-proliferative human cells. However, cancer cells the half-lives miRNAs comparatively short. To understand mechanism rapid miRNA turn over cells, we explored effect “usage” specific for translation repression their targets on abundance that miRNA. We have noted an accelerated extracellular vesicle mediated export “used” mammalian and miRNA-export process get retarded by Ago2 interacting protein GW182B. The GW182...
MicroRNAs, the tiny regulators of gene expression, can be transferred between neighbouring cells via Extracellular Vesicles (EV) to control expression genes in both donor and recipient cells. How Vesicles-derived miRNAs are getting internalized become functional target is an unresolved question. We found that EV-associated primarily single stranded and, functional, get loaded with Ago proteins present without requiring host Dicer1. Following endocytosis miRNA-containing extracellular...
Abstract Leishmania donovani , the causative agent of visceral leishmaniasis, infects and resides within tissue macrophage cells mammalian host. It is not clear how parasite infected cross-talk with non-infected in infection niche to regulate process. Interestingly, miRNAs, regulatory small RNAs host, could get trafficked into out as part extracellular vesicles ensure exchange epigenetic signals can expression their target genes both donor recipient cells. for its survival host macrophage,...
Abstract MicroRNAs, the tiny regulators of gene expression, can be transferred between neighbouring cells via Extracellular Vesicles (EV) to control expression genes in both donor and recipient cells. How Vesicles-derived miRNAs are getting internalized become functional target is an unresolved question. We found that EV-associated primarily single stranded and, functional, get loaded with Ago proteins present without requiring host Dicer1. Following endocytosis miRNA-containing...