A5050132357- Heat shock proteins research
- Protein Structure and Dynamics
- Enzyme Structure and Function
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- Endoplasmic Reticulum Stress and Disease
- Hydrogen's biological and therapeutic effects
- Autophagy in Disease and Therapy
- Chemical Reactions and Isotopes
- Erythrocyte Function and Pathophysiology
- Ion Transport and Channel Regulation
- RNA regulation and disease
- 3D Printing in Biomedical Research
Weizmann Institute of Science
2023-2024
Hebrew University of Jerusalem
2022
Abstract The endoplasmic reticulum (ER) unfolded protein response (UPR) is tuned by the balance between proteins and chaperones. While reserve chaperones are known to suppress UPR transducers via their stress-sensing luminal domains, underlying structural mechanisms remain unclear. Cellular biophysical analyses established that ER chaperone AGR2 forms a repressive complex with domain of transducer IRE1β. Structural prediction, X-ray crystallography NMR spectroscopy identify critical...
Cell growth is driven by the acquisition and synthesis of both dry biomass water mass. In this study, we examine increase mass in T cell during growth. We found that T-cell characterized an initial phase slow cellular water, followed a second rapid content. To study origin gain, developed novel methodology call cold aqua trap-isotope ratio spectrometry, which allows analysis isotope composition intracellular water. Applying discovered glycolysis-coupled metabolism accounts on average for 11...
SUMMARY J-domain proteins (JDPs) constitute a large family of molecular chaperones that bind broad spectrum substrates, targeting them to Hsp70, thus determining the specificity and activating entire chaperone functional cycle. The malfunction JDPs is therefore inextricably linked myriad human disorders. Here we uncover novel mechanism by which recognize misfolded clients, present in class-A JDPs. Through newly-identified β-hairpin site, these detect changes protein dynamics at initial...
Abstract Cell growth is driven by the acquisition and synthesis of dry biomass water mass. This study examines increase in T cells during cell growth. We found that initiated a phase slow cellular water, followed second rapid content. To origin gain, we developed novel method, Cold Aqua Trap – Isotope Ratio Mass Spectrometry (CAT-IRMS), which allows analysis intracellular isotope composition. Applying CAT-IRMS, discovered glycolysis-coupled metabolic accounts on average for 11 femtoliter...
Abstract The metabolic pathways controlling naive CD8 + T (Tn) cell maturation following thymic egress remain mostly undefined. This is important because immature Tn are a major component of peripheral immune tolerance in newborns and under lymphopenia. In this study we demonstrate that TMRM, mitochondrial membrane potential marker, could be applied to rapidly identify an population the periphery. Applying marker perform proteomic analysis, show cells maintain accelerated methionine cycle...