A5040377353- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Algal biology and biofuel production
- ATP Synthase and ATPases Research
- Photoreceptor and optogenetics research
- Plant Stress Responses and Tolerance
- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Spectroscopy and Quantum Chemical Studies
- Protist diversity and phylogeny
- Light effects on plants
- Diatoms and Algae Research
- Plant responses to elevated CO2
- Metal-Catalyzed Oxygenation Mechanisms
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Electrocatalysts for Energy Conversion
- Lipid metabolism and biosynthesis
- Paleontology and Stratigraphy of Fossils
- Plant responses to water stress
- Plant Virus Research Studies
- Microbial Fuel Cells and Bioremediation
- Plant Parasitism and Resistance
- Coral and Marine Ecosystems Studies
- Enzyme Catalysis and Immobilization
Kyoto University
2016-2025
Japan Science and Technology Agency
2011-2024
Kyoto University of Education
2024
Osaka University
2021
ORCID
2020
Zero to Three
2011
Okayama University
2009
RIKEN Center for Advanced Photonics
2005
SPring-8
2004
Light-harvesting complexes (LHCs) are diversified among photosynthetic organisms, and the structure of photosystem I-LHC (PSI-LHCI) supercomplex has been shown to be variable depending on species organisms. However, structural evolutionary correlations red-lineage LHCs unknown. Here, we determined a 1.92-Å resolution cryoelectron microscopic PSI-LHCI isolated from red alga Cyanidium caldarium RK-1 (NIES-2137), which is an important taxon in Cyanidiophyceae. We subsequently investigated...
Abstract In the photosynthetic apparatus, a major target of photodamage is D1 reaction center protein photosystem II (PSII). Photosynthetic organisms have developed PSII repair cycle in which photodamaged selectively degraded. A thylakoid membrane-bound metalloprotease, FtsH, was shown to play critical role this process. Here, effect FtsHs degradation investigated Arabidopsis (Arabidopsis thaliana) mutants lacking FtsH2 (yellow variegated2 [var2]) or FtsH5 (var1). Because these are...
Abstract PsbP and PsbQ proteins are extrinsic subunits of photosystem II (PSII) participate in the normal function photosynthetic water oxidation. Both exist a broad range oxygenic organisms; however, their physiological roles vivo have not been well defined higher plants. In this study, we established analyzed transgenic tobacco (Nicotiana tabacum) plants which levels or were severely down-regulated by RNA interference technique. A plant that lacked showed no specific phenotype compared to...
Abstract PsbP, an extrinsic subunit of photosystem II (PSII), is a nuclear-encoded protein that optimizes the water-splitting reaction in vivo. In addition to higher plants have two genes for PsbP homologs (PsbP-like proteins [PPLs]) show significant sequence similarity cyanobacterial homolog (cyanoP); however, function PPLs has not yet been elucidated. this study, we characterized Arabidopsis (Arabidopsis thaliana) mutants lacking either PPLs, PPL1 and PPL2. Phylogenetic analysis suggests...
A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into cells. By optimizing pulse conditions, cells can be transformed without removing rigid silica-based cell walls, and high efficiency (about 4,500 per 10(8) cells) is achieved.
Abstract Photosynthetic light-harvesting complexes (LHCs) play a pivotal role in collecting solar energy for photochemical reactions photosynthesis. One of the major LHCs are fucoxanthin chlorophyll / c -binding proteins (FCPs) present diatoms, group organisms having important contribution to global carbon cycle. Here, we report 2.40-Å resolution structure diatom photosystem I (PSI)-FCPI supercomplex by cryo-electron microscopy. The is composed 16 different FCPI subunits surrounding...
Abstract Acaryochloris marina is one of the cyanobacterial species that can use far-red light to drive photochemical reactions for oxygenic photosynthesis. Here, we report structure A. photosystem I (PSI) reaction center, determined by cryo-electron microscopy at 2.58 Å resolution. The reveals an arrangement electron carriers and light-harvesting pigments distinct from other type centers. paired chlorophyll, or special pair (also referred as P740 in this case), a dimer chlorophyll d its...
Abstract Chloroplast development adapts to the environment for performing suitable photosynthesis. Brassinosteroids (BRs), plant steroid hormones, have crucial effects on not only growth but also chloroplast development. However, detailed molecular mechanisms of BR signaling in remain unclear. Here, we identify a regulator development, BPG4, involved light and signaling. BPG4 interacts with GOLDEN2-LIKE (GLK) transcription factors that promote expression photosynthesis-associated nuclear...
Fucoxanthin chlorophyll (Chl) a/c-binding proteins (FCPs) function as light harvesters in diatoms. The structure of a diatom photosystem II-FCPII (PSII-FCPII) supercomplex have been solved by cryo-electron microscopy (cryo-EM) previously; however, the FCPII subunits that constitute tetramers and monomers are not identified individually due to their low resolutions. Here, we report 2.5 Å resolution PSII-FCPII using cryo-EM. Two types tetrameric FCPs, S-tetramer, M-tetramer, different...
PsbO protein is an extrinsic subunit of photosystem II (PSII) and has been proposed to play a central role in stabilization the catalytic manganese cluster. Arabidopsis thaliana two psb O genes that express proteins; PsbO1 PsbO2. We reported previously mutant plant lacked ( psbo1 ) showed considerable growth retardation despite presence PsbO2 [Murakami, R., Ifuku, K., Takabayashi, A., Shikanai, T., Endo, Sato, F. (2002) FEBS Lett 523, 138–142]. In present study, we characterized functional...
Arabidopsis has three PsbQ-like (PQL) proteins in addition to the PsbQ subunit of oxygen-evolving complex PSII. Recent bioinformatic and proteomic studies suggested that two PQL proteins, PQL1 (At1g14150) PQL2 (At3g01440), might function chloroplast NAD(P)H dehydrogenase (NDH) complex; however, their molecular not been characterized. In this study, we examined NDH pql1 pql2 mutants. Post-illumination increases Chl fluorescence, which are caused by an NDH-dependent cyclic electron flow, were...
Diatoms have attracted interest as biofuel producers. Here, the contents of lipids and photosynthetic pigments were analyzed in a marine centric diatom, Chaetoceros gracilis. This diatom can be genetically engineered using our previously reported transformation technique has potential to produce valuable materials photosynthetically. Sustainable culture conditions for cost-effective production biological under autotrophic with atmospheric carbon dioxide investigated laboratory. A...
A 33‐kDa protein component of the oxygen‐evolving complex in photosystem II is essential for photosynthesis, and it has been believed that mutants with deletion this are not found higher plants. We report here first isolation an Arabidopsis thaliana mutant a defect one genes proteins, psb O, intact gene ( O2). This showed considerable growth retardation, suggesting there functional difference between O O2.
Extrinsic proteins of photosystem II (PSII) regulate the oxygen-evolving reaction performed at Mn cluster by controlling binding properties indispensable cofactors Ca(2+) and Cl(-). However, molecular mechanism underlying this regulation is not yet understood. We have investigated structural couplings extrinsic PsbO, PsbP, PsbQ higher plants with using Fourier transform infrared (FTIR) spectroscopy. Light-induced FTIR difference spectra upon S(1) --> S(2) transition were measured spinach...