A5070329847- Inflammasome and immune disorders
- interferon and immune responses
- Influenza Virus Research Studies
- Evolution and Genetic Dynamics
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Drug Transport and Resistance Mechanisms
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- COVID-19 Clinical Research Studies
- Genetics, Aging, and Longevity in Model Organisms
- Protein Structure and Dynamics
- Mosquito-borne diseases and control
- bioluminescence and chemiluminescence research
- HIV Research and Treatment
- Molecular spectroscopy and chirality
- thermodynamics and calorimetric analyses
- Porphyrin Metabolism and Disorders
- Heat shock proteins research
- Machine Learning in Bioinformatics
- Viral gastroenteritis research and epidemiology
- Trace Elements in Health
VIR Biotechnology (United States)
2024
University of California, San Diego
2015-2023
La Jolla Alcohol Research
2022
The Transporter Classification Database (TCDB; http://www.tcdb.org) is a freely accessible reference database for transport protein research, which provides structural, functional, mechanistic, evolutionary and disease/medical information about transporters from organisms of all types. TCDB the only classification adopted by International Union Biochemistry Molecular Biology (IUBMB). It consists more than 10 000 non-redundant systems with 11 citations, classified into over 1000 transporter...
The NLRP1 inflammasome is a multiprotein complex that potent activator of inflammation. Mouse NLRP1B can be activated through proteolytic cleavage by the bacterial Lethal Toxin (LeTx) protease, resulting in degradation N-terminal domains and liberation bioactive C-terminal domain, which includes caspase activation recruitment domain (CARD). However, natural pathogen-derived effectors activate human have remained unknown. Here, we use an evolutionary model to identify several proteases from...
Hosts have evolved diverse strategies to respond microbial infections, including the detection of pathogen-encoded proteases by inflammasome-forming sensors such as NLRP1 and CARD8. Here, we find that 3CL protease (3CL pro ) encoded coronaviruses, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), cleaves a rapidly evolving region human CARD8 activates robust inflammasome response. is required for cell death release pro-inflammatory cytokines during SARS-CoV-2 infection. We...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) evolution has resulted in viral escape from clinically authorized monoclonal antibodies (mAbs), creating a need for mAbs that are resilient to epitope diversification. Broadly neutralizing sufficiently potent clinical development and retain activity despite remain elusive. We identified human mAb, designated VIR-7229, which targets the receptor-binding motif (RBM) with unprecedented cross-reactivity all sarbecovirus clades,...
The LysE superfamily consists of transmembrane transport proteins that catalyze export amino acids, lipids and heavy metal ions. Statistical means were used to show it includes newly identified families including transporters specific for (1) tellurium, (2) iron/lead, (3) manganese, (4) calcium, (5) nickel/cobalt, (6) (7) peptidoglycolipids as well (8) one family electron carriers. Internal repeats conserved motifs identified, multiple alignments, phylogenetic trees average hydropathy,...
Heat Shock Factor 1 (HSF-1) is a key regulator of the heat shock response (HSR). Upon shock, HSF-1 binds well-conserved motifs, called Elements (HSEs), and drives expression genes important for cellular protection during this stress. Remarkably, we found that substantial numbers HSEs in multiple Caenorhabditis species reside within Helitrons, type DNA transposon. Consistent with Helitron-embedded being functional, upon they display increased RNA polymerase II occupancy up-regulation nearby...
The vertebrate eye was described by Charles Darwin as one of the greatest potential challenges to a theory natural selection stepwise evolutionary processes. While numerous transitions that led have been explained, some aspects appear be specific with no obvious metazoan precursor. One critical difference between and invertebrate vision hinges on interphotoreceptor retinoid-binding protein (IRBP, also known retinol-binding protein, RBP3), which enables physical separation specialization...
Viruses interact with the intracellular transport machinery to promote viral replication. Such host–virus interactions can drive host gene adaptation, leaving signatures of pathogen-driven evolution in genomes. Here, we leverage these genetic identify dynein activating adaptor, ninein-like (NINL), as a critical component antiviral innate immune response and target antagonism. Unique among genes encoding components active complexes, NINL has evolved under recurrent positive (diversifying)...
Abstract Hosts have evolved diverse strategies to respond microbial infections, including the detection of pathogen-encoded proteases by inflammasome-forming sensors such as NLRP1 and CARD8. Here, we find that 3CL protease (3CL pro ) encoded coronaviruses, SARS-CoV-2, cleaves a rapidly evolving region human CARD8 activates robust inflammasome response. is required for cell death release pro-inflammatory cytokines during SARS-CoV-2 infection. We further natural variation alters sensing ,...
ABSTRACT The NLRP1 inflammasome is a multiprotein complex that potent activator of inflammation. Mouse NLRP1B can be activated through proteolytic cleavage by the bacterial Lethal Toxin (LeTx) protease, resulting in degradation N-terminal domains and liberation bioactive C-terminal domain, which includes caspase activation recruitment domain (CARD). However, natural pathogen-derived effector activate human remains unknown. Here, we use an evolutionary model to identify several proteases from...
We introduce an intuitive particle-based model of time-symmetric quantum physics that gives a concrete description what goes on in the time between projective measurements system. The time-symmetry this means we ...Physical interpretations formulation mechanics, due to Aharonov, Bergmann, and Lebowitz are discussed terms weak values. most direct, yet somewhat naive, interpretation uses ...
Abstract Previous positive selection analyses on primate host restriction factors have helped to elucidate differences in adaptation viral infection. However, many of these studies been focused small gene families and genes known or suspected play a role as factors. With the genome sequencing numerous species, we are now capable comparing evolutionary profiles across whole genome. Using guides, characterize similarities between sets other with diverse functions provide insight new candidate...
Abstract Viruses interact with the intracellular transport machinery to promote viral replication. Such host-virus interactions can drive host gene adaptation, leaving signatures of pathogen-driven evolution in genomes. Here we leverage these genetic identify dynein activating adaptor, ninein-like (NINL), as a critical component antiviral innate immune response and target antagonism. Unique among genes encoding components active complexes, NINL has evolved under recurrent positive...