A5101570475- Radiopharmaceutical Chemistry and Applications
- Vascular Procedures and Complications
- Central Venous Catheters and Hemodialysis
- Ferroelectric and Piezoelectric Materials
- Monoclonal and Polyclonal Antibodies Research
- Medical Imaging Techniques and Applications
- Microwave Dielectric Ceramics Synthesis
- Liver Disease Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Medical Imaging and Pathology Studies
- Ginseng Biological Effects and Applications
- Hepatocellular Carcinoma Treatment and Prognosis
- Esophageal and GI Pathology
- Tracheal and airway disorders
- Nanoparticle-Based Drug Delivery
- Boron Compounds in Chemistry
- Photoreceptor and optogenetics research
- Advanced Memory and Neural Computing
- Nanoplatforms for cancer theranostics
- Dielectric properties of ceramics
- Sulfur Compounds in Biology
- Biliary and Gastrointestinal Fistulas
- Healthcare and Venom Research
- Advanced biosensing and bioanalysis techniques
- Vascular anomalies and interventions
National Institutes of Health
2023-2025
National Cancer Institute
2024-2025
Center for Cancer Research
2024-2025
Soonchunhyang University
2010-2023
Kyungpook National University
2012-2022
Indiana University School of Medicine
2019-2021
Chungnam National University
2005-2021
Soon Chun Hyang University Cheonan Hospital
2021
Korea University
2017-2020
Kolon Industries (South Korea)
2020
PURPOSE: Total mesorectal excision with pelvic autonomic nerve preservation has been reported to be an optimal surgery for rectal cancer. It minimizes local recurrence and sexual urinary dysfunction. The aim of this study was assess the safety total in terms voiding function males METHODS: We performed urine flowmetry using Urodyn® a standard questionnaire International Index Erectile Function Prostate Symptom Score before after 68 RESULTS: Significant differences mean maximal flow rate...
Abstract Radiopharmaceutical therapy (RPT) is a promising approach to treating solid tumors, but therapeutic advances are impeded by the lack of broadly expressed targets and shared molecular vulnerability across different tumor types. Here, we evaluate VLA-4 (integrin α4β1) as potential target for RPT in tumors use radiolabeled copper-64 ([64Cu]Cu-) copper-67 ([67Cu]Cu-CB-TE1A1P-PEG4-LLP2A) LLP2A, peptidomimetic ligand VLA-4, preclinical imaging testing. Expression ITGA4, gene encoding...
A bio-realistic artificial synapse integrated with a nanogenerator (NG), which can be used in neuromorphic systems, is demonstrated for self-powered biomedical devices this study. Biocompatible amorphous (Na0.5K0.5)NbO3 (NKN) films are grown on TiN/polyimide substrates to synthesize NKN memristors use as synapses. Various synaptic functions realized memristors, driven by pulse generator and an NG. The plasticity of the memristor results from oxygen vacancy movements changes shape filaments....
An artificial synapse that can perform both learning and memory functions was realized using an amorphous Ta2O5 memristor. A Pt/Ta2O5/TiN memristor, with film grown at 100 °C, exhibited reliable bipolar switching properties the various conductance states required for applications; in addition, it transmission of physiological synapses. Various other synaptic were also obtained from memristor by modulating input bias. The metaplasticity a synapse, which is preliminary-spike-enhanced function,...
Most nanoparticles show much higher uptake in mononuclear phagocyte system (MPS) organs than tumors, which has been a long-lasting dilemma nanomedicine. Here, we report an imaging strategy that selectively decreases MPS organ uptakes by utilizing the differential esterase activity tumors and other organs. When esterase-labile radiotracer loaded liposome was injected into body, radioactivity rapidly excreted from liver spleen after breakage of ester bond esterase. However, lipophilic...
Single-domain antibodies, or nanobodies (Nbs), are promising biomolecules for use in molecular imaging due to their excellent affinity, specificity, and fast clearance from the blood. Given short blood half-life, pairing Nbs with short-lived radioisotopes is desirable. Because fluorine-18 (18F) routinely used clinical imaging, it an attractive radioisotope Nbs. We report a novel sortase-based, site-specific 18F-labeling method applied three nanobodies. Labeled were synthesized either by...
Abstract Hydrogen sulfide (H 2 S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H S live animals is very challenging. Herein, we report first radioisotope‐based immobilization technique for detection, quantification, vivo endogenous S. Macrocyclic 64 Cu complexes that instantly reacted with gaseous to form insoluble CuS highly sensitive selective manner were prepared. The concentration biological samples was measured...
The Pt/KNbO3/TiN/Si (KN) memristor exhibits various biological synaptic properties. However, it also displays nonlinear conductance modulation with the application of identical pulses, indicating that should be improved for neuromorphic applications. abrupt change originates from inhomogeneous growth/dissolution oxygen vacancy filaments in KN film. a film is controlled by two mechanisms different rates: redox process fast rate and an diffusion slow rate. Therefore, linearity can if only one...
We report a pH-responsive gold nanoparticles-in-liposome hybrid nanostructure, which effectively combines the assembly and surface plasmon property changes of 'smart' nanoparticles enhanced systemic circulation tumor accumulation PEG-grafted liposomes.
Glypican-3 (GPC3) is overexpressed in hepatocellular carcinomas and hepatoblastomas represents an important therapeutic target but the biologic importance of GPC3 liver cancer unclear. To date, there are limited data characterizing biological implications knockout (KO) cancers that intrinsically express this target. Here, we report on development characterization GPC3-KO cell lines compare to them parental lines. variants were established HepG2 Hep3B using a lentivirus-mediated CRISPR/Cas9...
Abstract Several chemical and enzymatic methods have been used to link antibodies moieties that facilitate visualization of cognate targets. Emerging evidence suggests the extent labeling, dictated by type chemistry used, has a substantial impact on performance, especially in context for tumors vivo. These effects are particularly pronounced studies using small antibody fragments, such as single‐domain antibodies, or nanobodies. Here, we leverage new variety conjugation chemistry, based...
Abstract Autoradiography is used to study the distribution and binding of radioisotope tagged ligands in tissue at microscale among other applications. The technology has evolved since its inception when it analogue film exposure techniques with introduction digital imaging systems sensitive ionizing radiation. We report on development our Quantitative Particle Identification spectral autoradiography system (QPID), which based Timepix3 sensor a gamma detecting scintillation crystal. This...
<p>(A) Platelet counts weekly after [67Cu]Cu-LLP2A treatment. (B) Creatinine, (C) alanine aminotransferase (ALT), and (D) total bilirubin collected in animals 5 months treatment with 55.5 MBq (1.5 mCi) of or control (PBS). Unpaired t-test; ns = not significant.</p>
<p>Table of mouse models for each tumor type</p>
<div>Abstract<p>Radiopharmaceutical therapy (RPT) is a promising approach to treating solid tumors, but therapeutic advances are impeded by the lack of broadly expressed targets and shared molecular vulnerability across different tumor types. Here, we evaluate VLA-4 (integrin α4β1) as potential target for RPT in tumors use radiolabeled copper-64 ([<sup>64</sup>Cu]Cu-) copper-67 ([<sup>67</sup>Cu]Cu-CB-TE1A1P-PEG<sub>4</sub>-LLP2A) LLP2A,...
<p>Immunohistochemistry staining of human CD3 spleen and tumor (IMR-5) for detection CAR T-cell engraftment.</p>
<p>(A) Time course biodistribution study with [67Cu]Cu-LLP2A in tumor-bearing mice B16-F10 melanoma. (B) Muscle is used as reference tissue for non-specific uptake. Mixed-effects analysis Dunnett’s multiple comparisons test; ****P < 0.0001; ***P 0.001; **P 0.01; *P 0.05; ns = not significant.</p>
<p>(A) PET/CT imaging and (B) biodistribution study with [64Cu]Cu-LLP2A in osteosarcoma 143B-bearing mice. Muscle is used as reference tissue for non-specific uptake. One-way ANOVA Tukey’s multiple comparisons test; ****P < 0.0001.</p>
<p>Successful radiolabeling of LLP2A with copper-64 ([64Cu]Cu) for further imaging studies. Radiochemical purity [64Cu]Cu-LLP2A was confirmed to be >98% by (A) radio-thin-layer chromatography (radio-TLC) and (B) radio-high-performance liquid (radio-HPLC) analysis.</p>
<p>(A) PET/CT imaging, (B) biodistribution study, and (C) dosimetry analysis with [64Cu]Cu-LLP2A in melanoma B16-F10–bearing male C57BL/6J mice. Muscle is used as reference tissue for non-specific uptake. One-way ANOVA Tukey’s multiple comparisons test; ****P < 0.0001; **P 0.01. (D) Biodistribution study female mice B16-F10 melanoma. Most tissues show no difference between females males exception of the spleen kidney. Two-way Sidak’s ***P 0.001; *P 0.05.</p>
<p>Histologic evaluation of end-organs 30 days after [67Cu]Cu-LLP2A treatment. (A) H&E staining and (B) pathologic findings.</p>
Bifunctional chelators have been successfully used to construct (64)Cu-labeled radiopharmaceuticals. Previously reported with cross-bridged cyclam backbones various essential features such as high stability of the copper(II) complex, efficiency radiolabeling at room temperature, and good biological inertness radiolabeled along rapid body clearance. Here, we report a new generation propylene-cross-bridged chelator hybrid acetate/phosphonate pendant groups (PCB-TE1A1P) developed aim combining...