A5090640677- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Neonatal Health and Biochemistry
- Parvovirus B19 Infection Studies
- Prenatal Screening and Diagnostics
- HIV/AIDS Research and Interventions
- Erythropoietin and Anemia Treatment
- HIV Research and Treatment
- Fungal Infections and Studies
- Hepatitis B Virus Studies
- Child Nutrition and Water Access
- HIV-related health complications and treatments
- Toxin Mechanisms and Immunotoxins
- Epigenetics and DNA Methylation
- Pediatric health and respiratory diseases
- Hepatitis C virus research
- RNA modifications and cancer
- Hemoglobin structure and function
- Hepatitis Viruses Studies and Epidemiology
- Venomous Animal Envenomation and Studies
- Antifungal resistance and susceptibility
- Reproductive System and Pregnancy
- Parasites and Host Interactions
Chiang Mai University
2016-2025
Maharaj Nakorn Chiang Mai Hospital
2017
Institut Pasteur
2004-2007
Carnegie Mellon University
2007
Mahidol University
2005
Abstract α‐Thalassemia‐1 Southeast Asian (SEA) type is the most common genetic disorder in population. Couples who are both carriers have a 25% chance of conceiving Bart’s hydrops fetalis. Therefore, results from carrier screening and prenatal diagnosis frequently need to be available rapidly. A rapid technique for α‐thalassemia‐1 SEA was implemented. The used based on real‐time gap‐PCR high resolution melting (HRM) analysis amplified fragment using Rotor‐Gene 6000™. DNA samples...
Red cell indices and formulas have been established as simple, fast, inexpensive means for discrimination between the β-thalassemia (β-thal) trait iron deficiency. However, there were no reports of diagnostic reliability different red in β-thal from deficiency Thai population. The aim this study was to examine accuracy five [red blood (RBC) count, mean corpuscular volume (MCV), hemoglobin (Hb) (MCH), Hb concentration (MCHC), distribution width (RDW)] eight (Sirdah, Green & King, RDW Index,...
FGFR1 is a receptor tyrosine kinase deregulated in certain breast cancers (BCs) with poor prognosis.Although FGFR1-activated phosphorylation cascades have been mapped, the key genes regulated by BC are largely unclear.FOXQ1 an oncogenic transcription factor.Although we found that activation of robustly upregulated FOXQ1 mRNA, how regulates gene expression and whether essential for FGFR1-stimulated cell proliferation unknown.Herein, confirmed mRNA protein cells.Knockdown blocked...
Limited access to healthcare in remote areas poses a significant challenge for non-communicable disease (NCD) prevention. This study assessed the feasibility and acceptability of HealthD, telehealth platform enabling Community Health Leaders (CHLs) screen monitor NCD risks rural Thailand. From June November 2023, prospective was conducted Chiang Mai, Thailand, involving 120 adults (aged 30–70 years) with no prior diagnosis. Trained CHLs performed risk assessments diabetes mellitus (DM),...
We characterized here for the first time deletional HbH disease caused by a large novel α0-thalassemia deletion in 26-year-old Burmese pregnant woman. Capillary electrophoresis (CE) electropherogram revealed HbA2ABart's H, whereas, single-tube multiplex real-time PCR with EvaGreen and high-resolution melting (HRM) analysis diagnosis of three common --SEA, --THAI, --CR deletions showed negative result. Thus, ligation-dependent probe amplification (MLPA) was performed. The α-globin gene...
Summary Cytokines are involved in regulating HIV-1 infection. They also placental environment major components. We assessed the potential impact of infection and/or anti-retroviral drugs on cytokine profiles that may be controlling dissemination. Placental explants were obtained after elective caesarean section from anti-retroviral-treated HIV-1-infected pregnant women and non-infected women. The main cytokines for protein secretion supernatants 24-h culture uncultured mRNA expression...
Prevention and control of thalassemia requires simple, rapid, accurate screening tests for carrier couples who are at risk conceiving fetuses with severe thalassemia.Single-tube multiplex real-time PCR SYBR Green1 high-resolution melting (HRM) analysis were used the identification α-thalassemia-1 Southeast Asian (SEA) Thai type deletions β-thalassemia 3.5-kb gene deletion. The results compared those obtained using conventional gap-PCR. DNA samples derived from 28 normal individuals, 11...
β-Thalassemia (β-thal) and iron deficiency cause most microcytic anemias. Red cell indices formulas have been established as simple, fast, inexpensive in discrimination between these two hematological disorders school children. However, whether could be applied to diagnose β-thal trait adult Thai subjects is unclear. The aim of this study was examine the diagnostic accuracy five red [red blood (RBC) counts, mean corpuscular volume (MCV), hemoglobin (Hb) (MCH), Hb concentration (MCHC),...
Penicillium marneffei is a dimorphic fungus, which endemic in Southeast Asia and responsible for emerging opportunistic infections. Diagnosis of penicilliosis may be difficult when few yeast cells are present, while gold standard diagnosis technique requires long-term culture. In order to provide more rapid accurate diagnosis, we developed TaqMan real-time PCR detect identify P. DNA coding 5.8S rRNA purified clinical samples. All preparations could detected using specific primers probe. The...
Hb Constant Spring [Hb CS; α142, Term→Gln (TAA>CAA in α2)] is often missed by routine laboratory testing since its mRNA as well gene product are unstable and presented at a low level peripheral blood. This study aimed to analyze the efficacy of capillary electrophoresis (CE) high performance liquid chromatography (HPLC) for detecting quantifying CS 19 heterozygotes 14 homozygotes with 10 H-CS disease subjects who were detected molecular analysis. In CE electrophoregram, was seen zone 2...
The α(0)-thalassemia South-East Asian (SEA)-type deletion is the most common genetic disorder in population. Couples who are both carriers have a 25% chance of conceiving Bart's hydrops fetalis. Therefore, results from carrier screening and prenatal diagnosis frequently need to be available rapidly. aim this study was implement droplet digital polymerase chain reaction (ddPCR) for SEA-type deletion.The wild-type α-globin gene allele SEA were quantified DNA samples 20 normal individuals, 15...
High performance liquid chromatography (HPLC) on fresh lysates is the standard test for identification of thalassemia. Samples in form dried blood spot(s) (DBS) mailed to reference laboratories where HPLC available could be an alternative. Hemoglobin (Hb) DBS at day 1, 7, 15 and 30 were analyzed by compared those from whole 0. A 100% consistent interpretation β-thalassemia (β-thal) trait β-thal/Hb E disease between was observed when analyzing Hb A2 a level 2.7–9.9% conjunction with lower MCV...
To compare hematological parameters between deletional and nondeletional HbH diseases, to investigate the correlation levels within these 2 groups.Samples of 43 which included 39 --SEA/-α3.7, 4 - -SEA/-α4.2, 22 diseases (- -SEA/αcsα), were used in this study. Correlations groups analyzed.The disease had higher RBC counts, total Hb, pack cell volume (PCV), mean corpuscular Hb (MCH), concentration (MCHC), HbA, HbA2 than did disease. A negative counts was detected group disease, while a...
Red cell indexes and formulas have been established as simple, fast, inexpensive tools to differentiate β-thalassemia (β-thal) trait from iron deficiency anemia. However, none of them showed 100.0% sensitivity specificity. Moreover, one index may show greater specificity in population but is ineffective another population. This study evaluated the diagnostic reliability a combination two red [red blood (RBC) distribution width (RDW)] nine called '11T score' for differentiation β-thal anemia...
Thalassemia and glucose-6-phosphate dehydrogenase (G-6-PD) deficiency are genetic disorders that cause hemolytic anemia. In areas with high frequencies of both hematological disorders, coinheritance G-6-PD thalassemia can be found. Whether deficiency, coinherited thalassemia, enhances severe anemia is still unclear. Hematological parameters between carriers those without were compared. The was diagnosed in 410 blood samples from patients using a fluorescent spot test. levels hemoglobin (Hb),...
α0-Thalassemia (α0-thal) Chiang Rai (– –CR; NC_000016.10: g.144215_188843del) was identified as a novel 44.6 kb deletional type of α-thalassemia (α-thal), removing all α-like globin genes. However, little is known about the deleterious effects this genetic disorder, particularly when it combined with other types thalassemia. We performed molecular analysis – –CR deletion using gap-polymerase chain reaction (gap-PCR) in two independent families residing Phayao and Mai, Thailand, an unknown...
Gel electrophoresis and ethidium bromide staining are routine methods in molecular laboratories. However, they not ideally suited to large scale analyses clinical We used SYTO9 high resolution melting (HRM) for identification of the common β0-thalassemia (β0-thal) Southeast Asia including codons 17 (A>T), 41/42 (−TCTT) 71/72 (+A) mutations. Multiplex amplification refractory mutation system-polymerase chain reaction (MARMS-PCR) was performed on 102 blood samples that had Hb A2 levels between...
The α-thalassemia-1 (α-thal-1) Southeast Asian (– –SEA) type deletion, β-thalassemia (β-thal) and Hb E [β26(B8)Glu→Lys, GAG>AAG] are the most common genetic disorders in populations. Mean corpuscular volume (MCV) <80.0 fL with normal hemoglobin (Hb) is used for screening α- β-thal, a level of less than 25.0% predicting α-thal-1 trait. Thus, levels Hb, MCV A2/E were reviewed compared between SEA deletion co-inherited β-thal trait (n = 61), 102) or homozygous 13) 636), 544) 83), respectively....
Using a single tube multiplex real-time PCR with SYBR Green1 and high resolution melting (HRM) analysis in routine laboratory for detection of alpha-thalassemia-1 Southeast Asian (SEA) type deletion simultaneously Thai is costly.Criteria testing were set as follow: (1) MCV < or = 75 fL negative SEA deletion, beta-thalassemia homozygous HbE; (2) HbE trait level 21.5% a-thalassemia-1 deletion; (3) Hb Bart's HbH found on HPLC chromatogram but deletion.From May 2010 to October 2012, the number...
Regions with a high prevalence of α-thalassemia (α-thal) require simple, rapid, and accurate tests for carrier screening prenatal diagnosis. Diagnosis multiple deletions in single tube is necessary to clearly identify individuals α 0 -thalassemia the routine setting, especially at-risk couples. Therefore, we aimed develop single-tube multiplex real-time PCR EvaGreen high-resolution melting (HRM) analysis identification Southeast Asian (SEA), Thai Chiang Rai (CR) type deletions. The results...