A5012088953- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Carcinogens and Genotoxicity Assessment
- Radiation Effects and Dosimetry
- Mass Spectrometry Techniques and Applications
- Free Radicals and Antioxidants
- Metal complexes synthesis and properties
- Analytical Chemistry and Chromatography
- Cancer therapeutics and mechanisms
- Acute Lymphoblastic Leukemia research
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- RNA regulation and disease
- Chemical Synthesis and Analysis
- Enzyme Structure and Function
- Metal-Catalyzed Oxygenation Mechanisms
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- Cancer-related Molecular Pathways
- Plant Genetic and Mutation Studies
- Genomics, phytochemicals, and oxidative stress
National Institute of Standards
2003-2025
National Institute of Standards and Technology
2016-2025
Material Measurement Laboratory
2016-2025
Royal United Hospital
2013
Yale University
2013
Lancaster University
2013
Biotechnology Institute
2011
University of Maryland, Baltimore
1983-2011
National Institutes of Health
1984-2007
National Institute on Aging
2003-2007
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSubstrate specificity of the Escherichia coli Fpg protein formamidopyrimidine-DNA glycosylase: excision purine lesions in DNA produced by ionizing radiation or photosensitizationSerge Boiteux, Ewa Gajewski, Jacques Laval, and Miral DizdarogluCite this: Biochemistry 1992, 31, 1, 106–110Publication Date (Print):January 14, 1992Publication History Published online1 May 2002Published inissue 14 January...
Oxidative stress has been implicated in many diseases. The chief source of reactive oxygen species within the cell is mitochondrion. We have characterized a variety biochemical and metabolic effects inactivation mouse gene for mitochondrial superoxide dismutase (CD1- Sod2 tm1Cje ). mutant mice exhibit tissue-specific inhibition respiratory chain enzymes NADH-dehydrogenase (complex I) succinate dehydrogenase II), tricarboxylic acid cycle enzyme aconitase, development urine organic aciduria...
8-oxoguanine (8-oxoG), ring-opened purines (formamidopyrimidines or Fapys), and other oxidized DNA base lesions generated by reactive oxygen species are often mutagenic toxic, have been implicated in the etiology of many diseases, including cancer, aging. Repair these all organisms occurs primarily via excision repair pathway, initiated with their glycosylase/AP lyases, which two classes. One class utilizes an internal Lys residue as active site nucleophile, includes Escherichia coli Nth...
Mixtures of Cu2+ and H2O2 at pH 7.4 caused damage to the bases in DNA greater than that by mixtures Fe3+ H2O2. Addition ascorbic acid Cu2+/H2O2 system a very large increase base damage, much produced Fe3+/H2O2/ascorbic system. The products presence were typical have been shown result from attack hydroxyl radicals upon bases. Cytosine glycol, thymine 8-hydroxyadenine especially 8-hydroxyguanine major both Cu2+/H2O2/ascorbic systems. Base these systems was inhibited chelating agents EDTA...
Engineered nanoparticles, due to their unique electrical, mechanical, and catalytic properties, are presently found in many commercial products will be intentionally or inadvertently released at increasing concentrations into the natural environment. Metal- metal oxide-based nanomaterials have been shown act as mediators of DNA damage mammalian cells, organisms, even bacteria, but molecular mechanisms through which this occurs poorly understood. For first time, we report that copper oxide...
Damage to the bases in DNA produced by hypoxanthine/xanthine oxidase system presence of iron ions was studied. The base products were measured using gas chromatography-mass spectrometry with selected ion monitoring after acidic hydrolysis and trimethylsilylation. Products identified cytosine glycol, thymine 5,6-dihydroxycytosine, 4,6-diamino-5-formamidopyrimidine, 8-hydroxyadenine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine, 8-hydroxyguanine. These are typical hydroxyl radical-induced DNA....
Incubation of a number ferric ion chelates with H2O2 at pH 7.4 generated reactive species able to produce chemical modifications the bases in DNA that are very similar those produced by hypoxanthine/xanthine oxidase system (Aruoma, O.I., Halliwell, B., and Dizdaroglu, M. (1989) J. Biol. Chem. 264, 13024-13028). Products were identified quantitated use gas chromatography-mass spectrometry selected-ion monitoring. Compared other complexes used, ion-nitrilotriacetic acid far largest amount base...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSubstrate specificity of the Escherichia coli endonuclease III: Excision thymine- and cytosine-derived lesions in DNA produced by radiation-generated free radicalsMiral Dizdaroglu, Jacques Laval, Serge BoiteuxCite this: Biochemistry 1993, 32, 45, 12105–12111Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November...
Free radical‐induced damage to DNA in vivo is implicated play a role carcinogenesis. Evidence exists that by endogenous free radicals occurs vivo, and there steady‐state level of radical‐modified bases cellular DNA. We have investigated levels typical base modifications chromatin various human cancerous tissues their cancer‐free surrounding tissues. Five different types surgically removed were used, namely colon, stomach, ovary, brain lung In samples isolated from these tissues, five...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTModification of DNA bases in mammalian chromatin by radiation-generated free radicalsEwa Gajewski, Govind Rao, Zeena Nackerdien, and Miral DizdarogluCite this: Biochemistry 1990, 29, 34, 7876–7882Publication Date (Print):August 28, 1990Publication History Published online1 May 2002Published inissue 28 August 1990https://pubs.acs.org/doi/10.1021/bi00486a014https://doi.org/10.1021/bi00486a014research-articleACS PublicationsRequest reuse...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFormation of 8-hydroxyguanine moiety in deoxyribonucleic acid on .gamma.-irradiation aqueous solutionMiral DizdarogluCite this: Biochemistry 1985, 24, 16, 4476–4481Publication Date (Print):July 30, 1985Publication History Published online1 May 2002Published inissue 30 July 1985https://pubs.acs.org/doi/10.1021/bi00337a032https://doi.org/10.1021/bi00337a032research-articleACS PublicationsRequest reuse permissionsArticle...
To protect cells from oxidative DNA damage and mutagenesis, organisms possess multiple glycosylases to recognize the damaged bases initiate Base Excision Repair pathway. Three have been identified in mammals that are homologous Escherichia coli Fpg Nei proteins, Neil1, Neil2, Neil3. Neil1 Neil2 human mouse well characterized while properties of Neil3 protein remain be elucidated. In this study, we report characterization Mus musculus (house mouse) (MmuNeil3) as an active glycosylase both...
The oxidatively induced DNA lesions 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) and 4,6-diamino-5-formamidopyrimidine (FapyA) are formed abundantly in of cultured cells or tissues exposed to ionizing radiation other free radical-generating systems. In vitro studies indicate that these miscoding, can block the progression polymerases, substrates for base excision repair. However, no study has yet addressed how metabolized cellular extracts. synthesis oligonucleotides containing FapyG...